Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 52 issue 3 (march 2018) : 337-342

Evaluation of one-shot vaccination protocol for suppressing reproductive functions in rams using encapsulated ovalbumin-LHRH-7 protein.

Ayhan Yilmaz, Ferda Karakus, Bünyamin Hakan, Keith Stormo, Firat Cengiz, Hasan Ülker.
1<p>Department of Animal Science, Agricultural Faculty, Siirt University 56000, Siirt, Turkey</p>
Cite article:- Yilmaz Ayhan, Karakus Ferda, Hakan B&uuml;nyamin, Stormo Keith, Cengiz Firat, &Uuml;lker. Hasan (2017). Evaluation of one-shot vaccination protocol for suppressing reproductive functions in rams using encapsulated ovalbumin-LHRH-7 protein. . Indian Journal of Animal Research. 52(3): 337-342. doi: 10.18805/ijar.v0iOF.8472.

ABSTRACT

The objective of study were to determine the effectiveness of Ovalbumin-LHRH-7 (OL) protein administered with cytosine guanine (CpG) adjuvant and Incomplete Freund’s Adjuvant (IFA), and used one-shot immunization (single-dose vaccination) protocol in which booster dose included in microspheres in rams. Fifty ram lambs at about a year old were used. Treatment groups receiving Ovalbumin LHRH and control contained 10 animals. They were stratified according to age (weeks), live weight and scrotal circumference size, and were randomly assigned to five groups. Scrotal circumference, sexual activities and the numbers of rams having sperm in the ejaculate were affected from treatment (P<0.05) depending on the dose and vaccination protocol. However, immunization did not affect live weight changes in any treatment groups (P>0.05). Findings clearly demonstrate that the effects of OL immunization on reproductive traits in yearling rams were prominent when it was administered at higher dose and classical one primary and one booster immunization as free protein form. Also we observed that the effect of higher and single dose of OL protein in encapsulated form on reproductive traits had the partial suppressing. CpG adjuvant along with IFA was proved to be an effective adjuvant and could be suggested to be used and alternative to FCA in hormone immunization. 

REFERENCES


  1. Bilskis, R. and N. Sutkeviciene. 2012. Effect of active immunization against GnRH on testosterone concentration, libido and sperm quality in mature AI boars. Acta Vet Scand., 54:33

  2. Bode, C., Zhao, G., Steinhagen, F., Kinjo, T. and D. M. Klinman. 2011. CpG DNA as a vaccine adjuvant. Expert Rev Vaccines 10: 499-511. 

  3. Brown, B.W., Mattner, P. E., Carroll, P.A., Holland, E. J., Paull, D.R., Hoskinson, R.M. and R.D.G. Rigby. 1994. Immunization of sheep against GnRH early in life. effects on reproductive function and hormones in rams. J. Reprod. Fertil., 10: 15–21.

  4. Conforti, V.A., de Avila, D.M., Cummings, N.S., Wells, K.J., Ülker, H. and J.J. Reeves. 2007. The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization. Vaccine 25 (35): 6537-6543.

  5. Conforti, V.A., de Avila, D.M., Cummings, N.S., Zanella, R., Wells, K.J., Ülker, H. and J.J. Reeves. 2008. CpG motif-based adjuvant as a replacement for Freund’s complete adjuvant in a recombinant LHRH vaccine. Vaccine 26 (7): 907-913.

  6. Ferro, V.A., Khan, M.A., McAdam, D., Colston, A., Aughey, E., Mullen, A.B., Waterson, M.M. and M.J. Harvey, 2004. Eûcacy of an anti-fertility vaccine based on mammalian gonadotrophin releasing hormone (GnRH-I) – a histological comparison in male animals. Vet. Immunol. Immunopathol., 01: 73–86.

  7. Godfrey, S.I., Walkden-Brown, S.W., Martin, G.B. and E.J. Speijers. 1996. Immunization of goat bucks against GnRH to prevent seasonal reproductive and agonistic behaviour. Anim. Reprod. Sci., 44: 41–54.

  8. Kerketta, S., Singh,M. Patel B.H.M., Verma, M., Prasad, J.K. Upadhyay, D., B. and B. Bhushan. (2014). Study on buck’s mating behavýour, libido score and semen Biology in local goat of Rohilkhand region, Ýndýa. Indian J. Anim. Res., 48 (5) : 491-495. 

  9. Kiyma, Z., Adams, T.E., Hess, B.W., Riley, M.L., Murdoch, W.J. and G.E. Moss,. (2000). Gonadal function, sexual behaviour, feedlot performance, and carcass traits of ram lambs actively immunised against GnRH. J. Anim. Sci., 78: 2237–2243.

  10. Reeves, J.J., Chang, C.F., De Avila, D.M., Grieger, H.E., Johnson, H.E. and A.J. Roberts. 1989. Vaccine against endogenous hormones. A possible future tool in animal production. J. Dairy Sci., 72: 3363-3371.

  11. Robertson, I.S. Fraser, H.M., Innes, G.M. and A.S. Jones. (1982). Effects of immunocastration on sexual and production characteristics of male cattle. Vet. Rec., 111: 529–531.

  12. Sarmah, B.K., Dutta, D.J., Sarmah, B.C. and R.N. Goswami. (1997). Influence of age on testicular development and body weight in crossbred buck. Indian J. Anim. Res., 31 (2): 107-112.

  13. Seghatoleslam, A., Ferro, A.V., Mansourian, M., Kargar, M., Hosseini, M.H., Manavian, M., Gebril, M., Mostafavi-Pour, Z. And M. Namavari. (2014). Evaluation of immunocastration conjugates based on GnRH linked to carrier molecules in a male rodent model. Comp Clin Pathology 23: 805–811.

  14. Senger, P L. 1997. Reproductive behavior. In. Pathways to Pregnancy and Parturation, Current Conceptions Inc., Pullman, pp. 188–205.

  15. Sun H X., Xie, Y. and Y.P. Ye. (2009). Advances in saponin-based adjuvants. Vaccine 27(12): 1787-96. 

  16. Ülker, H., Kanter, M., Gökdal, Ö., Aygün, T., Karakuº, F., Sakarya, M. E., deAvila, D.M. and J.J. Reeves. (2005). Testicular development, ultrasonographic and histological appearance of the testis in ram lambs immunized against recombinant LHRH fusion proteins. Anim. Reprod. Sci. 86 (3-4): 205-219.

  17. Ülker, H., Yilmaz, A., Karakuº, F., Yörük, M., Budað, C., deAvila, D. M. and J. J. Reeves,.(2009b). LHRH fusion protein immunization alters testicular development, ultrasonographic and histological appearance of ram testis. Reprod. Domes. Anim. 44: 593–599.

  18. Ülker, H., Gökdal, Ö., Aygün, T., Karakuº, F., De Avila, D.M. and J.J. Reeves. 2003. Feedlot performance and carcass characteristics of ram lambs immunized against recombinant LHRH fusion proteins at 10 weeks of ages. Small Rumin. Res., 50: 213-218.

  19. Ülker, H., Küçük, M. Yilmaz, A., Yörük M., L. Arslan, D.M. deAvila and J.J. Reeves. (2009a). Changes in testicular development, ultrasonographic and histological appearance of the testis in buck kids immunized against LHRH using recombinant LHRH fusion protein. Reprod. Domes. Anim. 44: 37–43.

  20. Ülker, H., Gökdal, Ö., Temur, C., Budað, C., Oto, M., de Avila D. M. and J.J. Reeves, (2002). The effects of immunization against LHRH on body growth and carcass characteristics in Karakaº ram lambs. Small Rumin. Res., 45 (3): 273-278.

  21. Ülker, H., Kanter, M., Gökdal, Ö., de Avila, D.M. and J.J. Reeves. (2001). The effects of recombinant LHRH fusion proteins on testicular development and histology in ram lambs. Berl Munch Týerarztl., 108: 459-464.

  22. Zhang, Y., Rozell, T.G., de Avila D.M., Bertrand, K. P. and J.J. Reeves. (1999). Development of recombinant ovalbumin-luteinizing hormone releasing hormone as a potential sterilization vaccine. Vaccine 17: 2185-2191. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)