Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 5 (october 2017) : 851-855

Analysis of the polymorphism of expressed sequence tag-Simple sequence repeat in quail

Jun Yan Bai*, You Zhi Pang, Yan Xia Qi, Xiao Hui Zhang, Yin Xian Yun
1<p>College of Animal Science and Technology,&nbsp;Henan University of Science and Technology, Luoyang 471003, China.&nbsp;</p>
Cite article:- Bai* Yan Jun, Pang Zhi You, Qi Xia Yan, Zhang Hui Xiao, Yun Xian Yin (2016). Analysis of the polymorphism of expressed sequencetag-Simple sequence repeat in quail . Indian Journal of Animal Research. 51(5): 851-855. doi: 10.18805/ijar.v0i0f.3803.

ABSTRACT

Aiming at accelerating the application of molecular markers in the genetic improvement of quails, six EST-SSR markers were successfully developed using a bioinformatics method. Polymorphisms of three quail populations (Chinese yellow, China black and Korean quail) were detected. The results showed that there were 2-6 alleles in six EST-SSR markers. The mean polymorphism information contents of Chinese yellow , China blackand Korean quail were 0.5451, 0.4962 and 0.4937, respectively. The average heterozygosity values were 0.6134, 0.5759 and 0.5613. Among the six EST-SSR markers, three were highly polymorphicand the others were moderately polymorphic. The newly-developed six EST-SSR markers may be used to determine the genetic diversity of quails. The six EST-SSR markers identified were related to carbohydrate metabolism and melanin synthesis, but the specific mechanisms need to be further analyzed.

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