Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Research Article

Indian Journal of Animal Research, volume 50 issue 6 (december 2016) : 893-899

Identification of peptides from sinus gland extracts in the red swamp crayfish Procambarus clarkii by mass spectrometry

Yan Shui, Zeng-Hong Xu, Xin Zhou*
1<p>Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture,&nbsp;<br /> Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.</p>
Cite article:- Shui Yan, Xu Zeng-Hong, Zhou* Xin (2016). Identification of peptides from sinus gland extracts in the red swampcrayfish Procambarus clarkii by mass spectrometry . Indian Journal of Animal Research. 50(6): 893-899. doi: 10.18805/ijar.11459.

ABSTRACT

Crustacean sinus gland (SG) is a well-defined neuroendocrine organ that controls the secretion of various neuropeptides which regulate many physiological activities. The red swamp crayfish Procambarus clarkii is a decapod crustacean with both high economic and scientific importance in China. To facilitate physiological investigations of SG peptide/hormone function in this species, we  have been employed tissue extract fractionation by HPLC to obtain a complete description of neuropeptidome and used Electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS) to detect the peptidome present in its organ. In total, 48 peptide sequences belonging to several known neuropeptide families including crustacean cardioactive peptide, CHH precursor-related peptides (CPRPs), orcokinins and pigment dispersing hormones were identified. Among these 48 sequences, 26 are novel peptides and 22 are previously identified. Overall, the results give a stimulus for future physiological studies of SG neuropeptides in P. clarkii and other crustaceans.

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