Histological and histochemical structure of lingual salivary glands in mole rat (Spalax leucodon)

DOI: 10.18805/ijar.v0iOF.4546    | Article Id: B-519 | Page : 252-255
Citation :- Histological and histochemical structure of lingual salivaryglands in mole rat (Spalax leucodon) .Indian Journal Of Animal Research.2017.(51):252-255

Nilgün Kuru*, Kenan Çinar, Emel Demirbag and Ramazan Ilgün

nkuru@cumhuriyet.edu.tr
Address :

Department of Anatomy, Faculty of Veterinary Medicine, Cumhuriyet University, TR-58140, Sivas, Turkey.

Submitted Date : 2-05-2016
Accepted Date : 2-09-2016

Abstract

The histological and histochemical structure of lingual salivary glands in mole rat (Spalax leucodon) were studied using histochemical staining techniques to provide information of its salivary glands. A total of five adult mole rat (Spalax leucodon) were used as the material. It was observed that serous and mucous glands are placed in the root of the tongue. It was detected that although the mucous gland cells were rich in terms of AB pH 0.5, 1.0, 2.5 and AF (+) mucosubstance, the PAS and KOH/PAS mucosubstances showed very weak reaction, and this mucosubstances were present at a very less amount in serous gland cells.  In the PAS/AB staining, it was found that the serous and mucous gland cells showing only AB or only PAS reaction and also AB AB pH 2.5 (+) cells were found. The same findings were observed in serous glands by AF/AB staining. Consequently, the present study demonstrated characteristic features of the lingual salivary glands of the mole rat, and it revealed histological and histochemical data both in accordance with and different from that for the lingual salivary glands of mammals and other species.

Keywords

Histochemistry Mucosubstance Mole rat (Spalax leucodon) Salivary gland Tongue.

References

  1. Bancroft JD, Stevens A and Turner DR (1996). Theory and Practice of Histological Techniques, Churchill , Livingstone. PP: 101.
  2. Branco E, Pereira WLA, De Lima AR, Franciolli ALR, Rici REG, Miglino MA, Muniz JAPC and Imbeloni A (2012). Ultrastructural aspects of Callithrix penicillata lingual papillae. Microscopy Research and Technique, 75: 282-284.
  3. Budak A, Mermer A, Göçmen B and Kaya U (2004). Systematics of Vertebrates, Ege University, Izmir. PP: 215.
  4. Cimenoglu N and Cinar K (2010). Following the incubation period, some of the stages of incubation and quails (Coturnix coturnix japonica) of the language development of histological and histochemical structure glands. Firat University Saglik Bilimleri Tip Derg, 24: 35-41.
  5. Culling CFA, Reid PE and Dunn WL (1976). A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids. J Histochem Cytochem, 24: 1225-1230.
  6. El-Bakry AM (2010). Study by transmission and scanning electron microscopy of the morphogenesis of three types of lingual papillae in the albino rat (Rattus rattus). Acta Zoologica (Stockholm), 91: 267-278.
  7. El-Kordy EA, Alanazi AD, Ali SS, Makhlou MMM and Rabah SO (2014). Histological, histochemical and ultrastructural changes in the submandibular gland of starved young male cats. J Cytol Histol, doi: 10.4172/2157-7099.1000252.
  8. Field RB, Spielman AI and Hand AR (1989). Purification of lingual amylase from serous glands of rat tongue and characterization of rat lingual amylase and lingual lipase. J Dent Res, 68: 139-145.
  9. Girgin A, Alabay, B, Liman N, Ozfiliz N, Gulmez N, Ozcan Z, Yoruk M, Erdost H, Aslan S, Ergun L and Zik B (2010). Veterinary Specific Histology. Nobel Med Pub, Istanbul. PP: 155.
  10. Gomari G (1952). Gomari’s aldehyde fuchsin stain. In [Culling CFA, Allison RT, Barr WT (Eds)]: Cellular Pathology Tecnique, Butterworths, London, PP: 238.
  11. Guyton AC and Hall JE (2007). Textbook of Medical Physiology. Nobel Med Pub, Istanbul. PP: 793
  12. Hamosh M, Klaeveman HL, Wolf RO and Scow RO (1975). Pharyngeal lipase and digestion of dietary triglyceride in man. J Clin Investigation, 55: 908–913.
  13. Harris HR (1900). On the rapid conversion of haematoxylin into haematein in staining reactions. J Appl Microsc, 3: 777-780.
  14. Kilinc M, Erdogan S, Ketani S and Ketani MA (2010). Morphological study by scanning electron microscopy of the lingual papillae in the middle east blind mole rat (Spalax ehrenbergi, Nehring, 1898). Anat Histol Embryol, 39: 509-515.
  15. Kuru M (2001).Vertebrates. Palme Publication, PP: 645.
  16. Leinonen J, Parkkila S, Kaunisto K, Koivunen P and Rajaniemi H (2001). Secretion of carbonic anhydrase isoenzyme VI (CA VI) from human and rat lingual serous von Ebner’s glands. J Histochem Cytochem, 49: 657–662. 
  17. Lev R and Spicer SS (1964). Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem, 12: 309.
  18. Maala C and Lianto E (2011). Histological and histochemical characterization of the lingual glands of the philippine water buffalo, Bubalus bubalis (Linnaeus, 1758) (Artiodactyla: Bovidae). Philipp J Vet Med, 48: 16. 
  19. Mc Manus JFA (1948). Histological and histochemical uses of periodic acid. Stain Technol, 23: 99-108.
  20. Mowry RW (1956). Alcian blue tecniques for the histochemical study of acidic carbohydrates. J Histochem Cytochem, 4: 407-408. 
  21. Okada H, Kanno T, Tamamura R, Nakada H, Sasaki Y, Kaneda T, Endoh M, Wakamatsu Y and Suzuki K. Histological features of the submandibular glands in the gray short-tailed opossum (Monodelphis domestica). Journal of Hard Tissue Biology, 23: 357-362.
  22. Pedini V, Gargiulo AM and Cercarelli P (1997). Basic and lectin histochemical characterization of bovine gustatory (von Ebner’s) glands. Anat Histol Embryol, 26: 223-226.
  23. Proctor GB (2016). The physiology of salivary secretion. Periodontology 2000, 70: 11-25.
  24. Spicer SS and Mayer DR (1960). Aldehyde fuchsin/Alcian blue. In: [Culling CFA, Allison RT, Barr WT]. Cellular Pathology Tecnique. London: Butterworths, PP:233.
  25. Taib N T and Jarrar BM (2001). Histochemical Characterization of the Lingual Salivary Glands of the Eurasian Collared Dove, Streptopelia decaocta. Pakistan J Biol Sci, 11: 1425-1428.
  26. Taib NT and Jarrar B M (2004). Histochemical Characterization of the Lingual Salivary Glands of the House Gecko, Ptyodactylus hasselquistii (Squamata: Gekkonidae). Asiatic Herpetol Res, 10: 176-181.
  27. Triantafyllou A, Fletcher D and Scott J (2001). Histochemical phenotypes of von Ebner’s gland of ferret and their functional implications. Histochem Jour, 33: 173-181.
  28. Unsaldi E (2010). Macroscopic and light microscopic structure of fungiform papillae on the tongue of squirrels (Sciurus vulgaris). Kafkas Univ Vet Fak Derg, 16: 115-118
  29. Yang C and, Wang L (2016). Histological and morphological observations on tongue of Scincella tsinlingensis (Reptilia, Squamata, Scincidae). Micron, 80: 24-33.

Global Footprints