Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 51 issue 3 (june 2017) : 525-530

Investigation of Rotavirus infection in sheep using serological and molecular techniques

Volkan Yilmaz*, M. Ozkan Timurkan, Nuvit Coskun, Yakup Yildirim
1<p>Department of Virology, Kafkas University, Faculty of Veterinary Medicine, 36300 Kars, Turkey.</p>
Cite article:- Yilmaz* Volkan, Timurkan Ozkan M., Coskun Nuvit, Yildirim Yakup (2016). Investigation of Rotavirus infection in sheep usingserological and molecular techniques . Indian Journal of Animal Research. 51(3): 525-530. doi: 10.18805/ijar.11463.

ABSTRACT

In this study, serological and molecular research was conducted on the Rotavirus infection in domestic breeds of sheep at 2–3 years of age. The sheep included in the study were raised on small scale family units of less than 20 sheep per unit, in central Kars province and its districts (Susuz, Arpaçay, Kagizman and Selim) in the Northeast Anatolia region of Turkey. The blood and fecal samples were collected randomly from 450 sheep. They were analyzed for the presence of Rotavirus and the antibody against the virus using enzyme-linked immunosorbent assay (ELISA). The highest seropositive ratio (73.46%) was found in central Kars province. The seroprevalence of Rotavirus in sheep raised in the Kars region was determined to be 55.33%. Rotavirus was not detected in fecal samples with ELISA. Molecular detection of Rotavirus from fecal samples was done by reverse transcription polymerase chain reaction (RT-PCR) technique using specific generic primers for VP6 protein. Rotavirus could not be detected in RT-PCR. The data that were obtained showed that the infection spreads on small scale family farms. Based on this information, recommendations were made for controlling Rotavirus infection.

REFERENCES


  1. Abe, M., Ito, N., Masatani, T., Nakagawa, K., Yamaoka, S., Kanamaru, Y., Suzuki, H., Shibano, K., Arashi, Y. and Sugiyama, M. (2011). Whole genom characterisation of new bovine rotavirus G21P[29] and G24P[33] strains    provides evidence for interspecies transmission. J. Gen. Virol., 92: 952-960.

  2. Ali, Y.H., Khalafalla, A.I., Gaffar, M.E., Peenz, I. and Steel, A.D. (2008). Detection and isolation of group A rotavirus from camel calves in Sudan. Vet. Arhiv., 78: 477-48.

  3. Alkan, F., Gulyaz, V., Timurkan, M.O., Iyisan, S., Ozdemir, S. and Turan, N. (2012). A large outbreak of enteritis in goat flocks in Marmara, Turkey, by G8P[1] group A rotaviruses. Arch. Virol., 157: 1183-1187.

  4. Al-yousif, Y., Anderson, J., Chard-Bergstrom, C., Bustamant, A., Muenzenberger, M., Austin, K. and Kapil, S. (2001). Evaluation of a latex agglutination kit (virogenrota test) for detection of bovine rotavirus in fecal samples. Clin. Diagn. Lab. Immunol., 8: 496-498.

  5. Andres, S., Jimenez, A., Sanchez, J., Alonso, J.M., Gomeza, L., Lopez, F. and Rey, J. (2007). Evaluation of some etiological factors predisposing to diarrhoea in lambs in “La Serena” (Southwest Spain). Small Rumin. Res.,70: 272-275.

  6. Badaracco, A., Garaicoechea, L., Matthijnssens, J., Louge Uriarte Odeon, A., Bilbao, G., Fernandez, F., Parra, G.I. and Parreno, V. (2013). Phylogenetic analyses of typical bovine rotavirus genotypes G6, G10, P[5] and P[11] circulating in Argentinean beef and dairy herds. Infect. Genet. Evol.,18: 18-30.

  7. Burgu, I., Akca, Y., Alkan, F., Ozkul, A. and Karaoglu, T. (1999). The investigatioii of rotavirus infections in diarheic and non-diarrheic sheep using PAGE and ELISA. Ankara Üniv. Vet. Fak. Derg., 46: 143-147.

  8. Carsten, E.B. (2009). Ratification vote 10 taxonomic proposals to the International Committe on taxonomy of viruses. Arch. Virol., 155: 133-146.

  9. Chasey, D. and Banks, J. (1986). Replication of atypical ovine rotavirus in small intestine and cell culture. J. Gen. Virol.,67: 567-576.

  10. Chitambar, S.D., Tatte, V.S., Dhongde, R. and Kalrao, V. (2008). High frequency of rotavirus viremia in children with acute gastroenteritis: discordance of strains detected in stool and sera. J. Med. Virol., 80:2169-76.

  11. Ciarlet, M., Hoffmann, C., Lorusso, E., Baselga, R., Cafiero, M.A., Bányai, K., Matthijnssens, J., Parreño, V., de Grazia, S., Buonavoglia, C. and Martella, V. (2008). Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain. Virus Genes., 37: 250-265.

  12. Dennehy, P.H. (2008). Rotavirus vaccines: an overview.Clin. Microbiol. Rev., 21: 198–208.

  13. Dhama, K., Chauhan, R.S., Mahendran, M. and Malik, S.V.S (2009). Rotavirus diarrhoea in bovine and other domestic animals. Vet. Res. Commun., 33: 1-23.

  14. Estes M.K. and Kapikian, A.Z. (2007). Rotaviruses and their replication. In Fields Virology, 5th edn, pp. 1917-1974. Edited by BN Fiels, DM Knipe, PM Howley, DE Griffin, RA Lamb,MA Martin, B Roizman & SE Straus. Philadelphia, PA: Lippincott, Williams, Wilkins.

  15. Falcone, E., Tarantino, M., Di Trani, L., Cordioli, P., Lavazza, A. and Tollis, M. (1990). Determination of bovine rotavirus G and P serotypes in Italy by PCR. J. Clin. Microbiol., 37: 3879-3882, 1990.

  16. Galindo-Cardiel, I., Fernández-Jiménezc, M., Lujánd, L., Buesac, J., Espadae, J., Fantovae, E., Blancof, J., Segalésa, J. and Badiolad, J.J. (2011). Novel group A rotavirus G8 P[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs. Vet. Microbiol., 149: 467-471.

  17. Garaicoechea, L., Bok, K., Jones, L.R., Combessies, G., Odeon, A., Fernandez, F. and Parreno, V. (2006). Molecular characterization of bovine rotavirus circulating in beef and dairy herds in Argentina during a 10-year period (1994-2003). Vet Microbiol, 118: 1-11.

  18. Gazal, S., Taku, A.K. and Kumar, B. (2011). Predominance of rotavirus genotype G6P[11] in diarrhoeic lambs. Vet. J., 193: 299-300.

  19. Gokce, E. and Erdogan, H.M. (2009). An epidemiological study on neonatal lamb healty. Kafkas Univ. Vet. Fak. Derg., 15: 225-236.

  20. Gumusova, S.O., Yazici, Z., Albayrak, H. and Meral, Y. (2007). Rotavirus and corona virus prevalence in healthy calves and calves with diarrhea. Medycyna. Wet., 68: 62- 64.

  21. Gutiérrez-Aguirre, I., Steyer, A., Boben, J., Gruden, K., Poljsak-Prijatelj, M. and Ravnikar, M. (2008). Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription-Real-Time Quantitative PCR Assay. J. Clin. Microbiol., 46: 2547–2554.

  22. Holland, R.E. (1990). Some infectious causes of diarrhea in young farm animals. Clin. Microbiol. Rev., 3: 345-37.

  23. Hysser, J.M. and Estes, M.K. (2008). Rotavirus vaccines and pathogenesis: Curr. Opin. Gastroenterol., 25: 36-43.

  24. Iturriza-Gomara, M., Wong, C., Blome, S., Desselberger, U. and Gray J. (2002). Molecular characterization of VP6 genes of human rotavirus isolates: Correlations of genogroups with subgroups and evidence of independent- segregation. J. Virol., 76: 6596-6601.

  25. Kaminjolo, J.S. and Adesiyun, A.A. (1994). Rotavirus infection in calves, piglets, lambs and goat kids in Trinidad. Br. Vet. J., 150: 293-299.

  26. Khafagi, M.H., Mahmoud, M.A. and Habashi, A.R. (2010). Prevalence of rotavirus infections in small ruminants. Global Vet., 4: 539-543, 2010.

  27. Makabe T, Sato, K., Ouneda, S. and Inaba, Y. (1985). Haemagglutination with ovine rotavirus. Arch. Virol., 90: 153-158.

  28. Mayameei, A., Mohammadi, G., Yavari, S.A., Fishery, E. and Omidi, A. (2010). Evaluation of relationship between rotavirus and corona virus infection with calf diarrhea by capture ELISA. Comp. Clin. Pathol., 19: 533-557.

  29. Mukhopadhya, I., Sarkar, R., Menon, V., Babji, S., Paul, A., Rajendran, P., Sowmyanarayanan, T., Moses, P., Iturriza-    Gomara, M., Gray, J. and Kang, G. (2013). Rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative PCR. J. Med. Virol., 85: 1661–1668.

  30. Munoz, M., Alvarez, M., Lanza, I. and Carmenes, P. (1996). Role of enteric pathogens in the aetiology of neonatal diarrhoea in lambs and goat kids in Spain. Epidemiol. Infect.,117: 203-211.

  31. Schoenian, S. (2007). Diarrhea (scours) in small ruminants. Comp. Ext. J., 8: 112-116

  32. Tate JE., Mijatovic-Rustempasic, S., Tam, K.I., Lyde, F.C., Payne, D.C., zilagyi, P. S, Edwards, K., Staat, M.A., Weinberg, G.A., Hall,. C.B., Chappell, J., Mcneal, M., Gentsch, J.R., Bowen, M.D. and Parashar, U.D. (2013). Comparison of 2 Assays For Diagnosing Rotavirus And Evaluating Vaccine Effectiveness in Children With Gastroenteritis. Emerg. Infect. Dis., 19: 1245–1252.

  33. Theil K.W., Grooms, D.L., McCloskey, C.M. and Redman, D.R. (1995). Group B rotavirus associated with an outbreak of neonatal lamb diarrhea. J. Vet. Diagn. Invest., 7: 148-150.

  34. Wani, S.A., Bhat, M.A., Nawchoo, R., Munshi, Z.H. and Bach, A.S. (2004). Evidence of rotavirus associated with neonatal lamb diarrhea In India. Trop. Anim.Health. Prod., 36: 27-32

  35. Yildirim, Y., Bilge Dagalp, S., Tan, M.T. and Kalaycioglu, A.T. (2008). Seroprevalence of the Rotavirus and Corona Virus Infections in Cattle. J. Anim. Vet. Adv.,7: 1320-1323

     
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)