Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 51 issue 2 (april 2017) : 205-211

Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep 

Tamer S. Abdelmoneim, Peter H. Brooks, Mohamed Afifi*, Ayman Abdel-Aziz Swelum
1<p>School of Biological Sciences, Faculty of Science and Environment,&nbsp;Plymouth University, Plymouth, PL4 &nbsp;8AA, UK.</p>
Cite article:- Abdelmoneim S. Tamer, Brooks H. Peter, Afifi* Mohamed, Swelum Abdel-Aziz Ayman (2016). Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep . Indian Journal of Animal Research. 51(2): 205-211. doi: 10.18805/ijar.11457.

This study aimed to evaluate the relationship between growth hormone (GH) gene polymorphism and estimated body weight in Harri sheep. One hundred Harri sheep lambs were used to determine the birth weight (BW) and weight at 120 days of age. The daily live-weight gain (DLWG) 0-120 days (g) was calculated. The complete CDS of the Harri sheep GH gene is 2117pb in length (GenBank acc. no. KU255857). Three novel SNPs were detected by comparing with GenBanke acc. no. X12546_1. The G871A SNP in intron II, G1383A in exon IV that resulted in conversion of the amino acid arginine number 121 to lysine (R121K) and the A1509G in intron IV. Each SNP was found on both alleles the mutant homozygote was more common (48, 56 and 50%) than the heterozygote (30, 18 and 20%) for G871A, G1383A and A1509G respectively. A positive significant (P<0.05) correlation between growth traits (BW, 120 day body weight and DLWG) and SNP and a highly significant correlation with the genotype were detected. The regression analysis indicated the positive effect of genotype and SNPs on the growth traits. Individuals carrying homozygote mutant alleles had the heaviest body weight and the highest DLWG. Consequently, these SNP may be useful indicators in the selection of lambs for higher growth rate and meat production.

  1. Afifi, M., Metwali, E.M.R. and Brooks, P.H. (2014). Association between growth hormone single nucleotide polymorphism and body weight in four Saudi camel (Camelus dromedarius) breeds. Pak Vet J. 34:494-498.

  2. Aminafshar, M. and Fathi, A. (2012). Single nucleotide polymorphisms in intron 1 of growth hormone gene and it’s association with economic important traits in Iranian Fars native fowl. Ann Biol Res. 3:4028-4032

  3. Bastos, E., Cravador, A., Azevedo, J. and Guedes-Pinto, H. (2001). Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed “Churra da Terra Quente. Biotechnol Agron Soc Environ. 5: 7-15.

  4. Byrne, C., Wilson, B. and Ward, K. (1987). The isolation and characterization of the ovine growth hormone gene. J Biol Sci. 40: 459-468.

  5. Croquet, C., Mayeres, P., Gillon, A., Vanderick, S. and Gengler, N. (2006). Inbreeding depression for global and partial economic indexes, production, type and functional traits in the Walloon Region of Belgium. J Dairy Sci. 86: 2257-2267.

  6. Etherton, T. and Bauman, D. (1998). Biology of somatotropin in growth and lactation of domestic animals. Physiol Rev. 78: 745-761.

  7. FAO, Food and Agriculture Organisation, (2011). FAO STAT Available at:

  8. Ge, W., Davis, M., Hines, H., Irvin, K. and Simmen, R. (2003). Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle. J Anim Sci. 81: 641-648.

  9. Gul, F., Sadaf, S. and Akhter, M.W. (2009). Ovis aries breed lohi growth hormone gene, complete cds, NCBI, GenBank, accession number, GQ452268, 

  10. Ishag, I.A., Reissmann, M., Peters, K.J., Musa, L.M. and Ahmed, M.K. (2010). Phenotypic and molecular characterization of six Sudanese camel breeds. S Afr J Anim Sci. 40: 319-326. 

  11. Jia, J.L., Zhang, L.P., Wu, J.P., Ha, Z.J. and Li, W.W. (2014). Study of the correlation between GH gene polymorphism and growth traits in sheep. Genetics and Molecular Research. 13: 7190-7200.

  12. Malewa, A.D., Hakim, L., Maylinda, S. and Husain, M.H. (2014). Growth hormone gene polymorphisms of Indonesia fat tailed sheep using PCR-RFLP and their relationship with growth traits. Livestock Research for Rural Development. 26 : 2014.

  13. Maniou, Z. (2003). Molecular evaluation of pituitary growth hormone (AJ575419). PhD Thesis, Biological Sciences, University of Sussex, Brighton, UK.

  14. Maniou, Z., Wallis, O., Sami, A. and Wallis, M. (2001). Molecular evolution of growth hormone in Cetartiodactyla. Endocrine Abstr. 2:58 (Abstr.)

  15. Maniou, Z., Wallis, O. and Wallis, M. (2004). Episodic molecular evolution of pituitary growth hormone in Cetartiodactyla. J Mo. Evol. 58: 743-753.

  16. McMahon, C.D., Radcliff, R.P., Lookingland, K.J. and Tucker, H.K. (2001). Neuroregulation of growth hormone secretion in domestic animals. Dom Anim Endocrinol. 20: 65-87.

  17. Musa, L. (2007). Characterization and utilization of dairy cattle in Sudan,” PhD Thesis, Humboldt Universitat Zu Berlin, Germany.

  18. Neelam, G., Ahlawat, S., Kumar, D., Gupta, S., Alok, P. and Geetu, M. (2007). Single nucleotide polymorphism in growth hormone gene exon-4 and exon-5 using PCR-SSCP in Black Bengal goats – A prolific meat breed of India. Meat Sci. 76: 658-665.

  19. Nei, M. and Kumar, S. (2000). Molecular Evolution and Phylogenetics, Oxford Univ Press: New York, USA. pp.192-196. 

  20. Orian, J.M., O’Mahoney, V. J. and Brandon, R.M. (1988). Cloning and sequencing of the ovine growth hormone gene. Nucleic Acids Research.16: 90946.

  21. Parmentier, I., Portetelle, D., Gengler, N., Prandi, A., Bertozzi, C., Vleurick, I., Gilson, R. and Renaville, R. (1999). Candidate gene markers associated with somatotropic axis and milk selection. Dom Anim Endocrinol. 17:139-    148.

  22. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A laboratory manual, 2nd ed. 3 volumes. Cold Springs Harbor Laboratory, Cold Springs Harbor, NY

  23. Sodhi, M., Mukesh, M., Prakash, B., Mishra, B., Sobti, R., Karn, S., Singhand, S. and Ahlawat, S. (2014). MspI allelic pattern of bovine growth hormone gene in Indian Zebu cattle (Bosindicus) breeds. Biochem Genet. 45:145-153. 

  24. Svennersten-Sjaunja, K. and Olsson, K. (2005). Endocrinology of milk production. Dom Anim Endocrinol. 29: 241-258.

  25. Vacca, G.M., Dettori, M.L., Balia, F., Luridiana, S., Mura, M.C., Carcangiu, V. and Pazzola, M. (2013). Sequence polymorphisms at the growth hormone GH1/GH2-N and GH2-Z gene copies and their relationship with dairy traits in domestic sheep (Ovis aries). Mol Biol Rep. 40:5285–5294.

  26. Vize, P. and Wells, J. (1987). Spacer alterations, which increase the expression of porcine growth hormone in E. coli. FEBS Letters. 213:155-158.

  27. Wickramaratne, S.H.G., Ulmek, B.R., Dixit, S.P., Kumar, S. and Vyas, M.K. (2010). Use of growth hormone gene polymorphism in selecting Osmanabadi and Sangamneri goats. Trop. Agric. Res. 21:398-411.

  28. Zhang, Y., Xiraigol, U. and Dugarjav, M. (2004). Equuscaballus growth hormone gene, complete CDs (AY837571). Department of Biology, Inner Mongolia Agri Univ, Huhhot, Inner Mongolia, P R China.


Editorial Board

View all (0)