Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 56 issue 12 (december 2022) : 1562-1565

Hepatoprotective Effect of Carica papaya Leaves and Andrographis paniculata Plant Material in Cyclophosphamide Induced Male Wistar Rats

Pavan Goyal1,*, D.B. Mondal1, S. Dey1, K.P. Singh1, S.K. Dixit1, S. Mehrotra1, S.K. Maiti1, A.G. Telang1
1Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India.
Cite article:- Goyal Pavan, Mondal D.B., Dey S., Singh K.P., Dixit S.K., Mehrotra S., Maiti S.K., Telang A.G. (2022). Hepatoprotective Effect of Carica papaya Leaves and Andrographis paniculata Plant Material in Cyclophosphamide Induced Male Wistar Rats . Indian Journal of Animal Research. 56(12): 1562-1565. doi: 10.18805/IJAR.B-4961.
Cyclophosphamide is used for the treatment of cancer cells and for suppressing the immune system during implantation. It adversely affects the cancer cells as well as healthy cells and increases the oxidative stress in these cells so cellular proteins, lipids and nucleic acids get denatured. It also produces liver, spleen and bone marrow toxicity. Medicinal plants such as Carica papaya and Andrographis paniculata possess hemopoietic, thrombopoietic and hepato-renal protective properties. The current study aimed to study the phytoconstituents in the hydro-ethanolic extracts of Carica papaya leaves and Andrographis paniculata plant material and their hepatoprotective effect in cyclophosphamide induced male Wistar rats.The collected plant materials were washed, dried, pulverized into fine powder, stored in air-tight containers and subjected to quantitative estimation of phytoconstituents present in the hydro-ethanolic extracts of Carica papaya leaves and Andrographis paniculata plant material. The hepato-protective properties of hydro-ethanolic extracts of these plants were evaluated on the basis of serum biochemistry analyses. Total phenolic content, alkaloids, flavonoids, saponin and cardiac glycosides were present in higher amount in hydroethanolic extract of Carica papaya leaves and was found more hepatoprotective @ 200 mg/kg b. wt. compared to Andrographis paniculata.
The medicinal plants are widely used to treat livestock, human and pet animals and this idea is not new. Most of the active principles of medicines or drugs are originally extracted from plant materials. After extraction and purification, these medicinal plants serve as source of drugs (Mir et al., 2016).
 
Carica papaya (Family: Caricaceae) is the well-known plant in the world (Ong et al., 2011). It is a soft wooded single- stemmed perennial tree, 2-10 m in height, with a crown of large palmate from the apex of the trunk (Jiao et al., 2010). Its leaves contain secondary metabolites like flavonoids, alkaloids, saponins, tannins, β-carotene, glycosides and steroids having immunomodulatory, antitumor and antipathogenic activities (Anibijuwon and Udeze, 2009). Papaya leaves also having bioactive compounds such as chymopapain and papain that help in the process of digestion and also inhibit the growth of pathogenic micro-organisms (Unaeze and Brikwa, 1986; Yismaw et al., 2008). Papaya leaves are rich in protease and amylase. These enzymes have high anti-inflammatory properties which reduce the inflammation of stomach and colon (Alabi et al., 2012). Papaya leaves extract heals peptic ulcers by killing bacteria Helicobacter pylori owing to their antimicrobial properties. Papaya leaves contain carpain, acetogenin and phenolic compounds. Carpain is a chemical compound or a substance with ability to kill micro-organisms that often intervene in food digestion processes (Anibijuwon and Udeze, 2009; Calzada et al., 2007) and also stops the excess growth of skin flora by sanitizing skin from the toxins and provides protection against skin problems like pimples, freckles and acne (Udoh et al., 2005).
 
Andrographis paniculata is commonly called as “Kalmegh” (Family: Acanthaceae) and referred to as a medicinal plant (Saranya et al., 2010). It possesses various medicinal properties such as anti-malarial, anticancer, anti-inflammatory, antiulcer, anti-hyperglycemic, antiviral, anti-angiogenic, hepatoprotective, antimicrobial, immunomodulatory,  anti-parasitic and antioxidant properties (Chandrasekaran et al., 2009; Akbar, 2011; Ojha et al., 2012). Its plant extract contains diterpenes, flavonoids, xanthones, noriridoides etc. (Singha et al., 2010). It has anti-microbial/ protozoan, anti-inflammatory, anti-oxidant, immuno-stimulant, anti-diabetic, hepato-renal protective and liver enzymes modulator properties (Okhuarobo et al., 2014). Kalmegh is used for liver diseases, blood purifier and dermatological diseases (Prathanturarug et al., 2007). Keeping these aspects in mind, the proposed study has been designed for determination of phytoconstituents in Carica papaya leaves and Andrographis paniculata plant material extracts and their hepatoprotective effect in cyclophosphamide induced male Wistar rats. 
 
The present study was conducted at Indian Veterinary Research Institute, Izatnagar, Bareilly during the year 2021-2022. The plant materials were collected (around 5 kg each as per requirement) from Bareilly district of Uttar Pradesh province of India and were washed three times using distilled water to clean and remove the waste materials and shade dried until dryness. The dried plant materials were pulverized into fine powder and stored in air-tight containers to avoid contact with moisture. The required plant materials were defatted using petroleum ether as solvent, sonicated for 3 minutes and a pause of 30 seconds for 6 times using 50% ethanol prepared by 50 mL ethanol and 50 mL distilled water and centrifuged at 4000 rpm and 4°C for 12 minutes. The total phenolic content and total tannins in the extracts were measured by the method described by (Makkar et al., 1993) with little modifications. The total alkaloids, flavonoids, saponin and cardiac glycosides were estimated by the method described by the (Ajanal et al., 2018; Dwivedi et al., 2020; Mir et al., 2016 and Onyema et al., 2016), respectively. In this experiment, a total of thirty-six male Wistar rats were utilized by prior permission from Institutional Animal Ethics Committee and they were categorized in six groups having six rats in each (Table 1). Blood samples were collected aseptically in vacutainers without anticoagulant on day 14 and serum biochemistry was performed according to the procedures. The data obtained from various treatments under study were analysed by using student’s “t” test and repeated measures “ANOVA”.                
 

Table 1: Experiment protocol.


 
In the present study, the total phenolic content, total tannins, total alkaloids, total flavonoids, total saponin and total cardiac glycosides in hydro-ethanolic extract of Carica papaya leaves were 44.27±1.50 mg TAE/g of dry weight, 17.76±5.13%, 29.80±1.55 mg/g, 6.63±0.20 mg GAE/g of dry weight, 1.31±0.01% and 3.08±0.01%, respectively whereas in hydro-ethanolic extract of Andrographis paniculata plant material were 33.51±1.66 mg TAE/g of dry weight, 11.84±2.96%, 15.71±1.02 mg/g, 3.93±0.53 mg GAE/g of dry weight, 1.01±0.01% and 2.15±0.09%, respectively (Table 2).
 

Table 2: Mean±SE values of phytoconstituents present in H-E extracts.


 
There was significant (P<0.05) increased in aspartate aminotransferase (AST) level in group-II but no significant difference (P>0.05) in group-III, group-IV, group-V and group-VI; significant (P<0.05) increased in alanine aminotransferase (ALT) level in group-II and group-V but no significant difference (P>0.05) in group-III, group-IV and group-VI and significant (P<0.05) increased in alkaline phosphatase level in group-II and group-V but no significant difference (P>0.05) in group-III, group-IV and group-VI compared to group-I (Table 3). Zhang et al., (2021) reported that the ALT level was significantly greater (P<0.01), but the AST level was somewhat reduced. The leaching out of enzymes of liver damage (ALT, AST and alkaline phosphatase) from the hepatocytes, resulting in enhanced enzyme activity in the systemic circulation, is one of the effects of cyclophosphamide-induced hepatic injury (Senthilkumar et al., 2006). 
 

Table 3: Serum biochemistry of different groups of male Wistar rats (Mean±S.E.).


 
Mohammed et al., (2011) reported that on administration of the aqueous Carica papaya leaf extract in a dose-dependent manner in male Wistar rats, there was a significant (P<0.05) restoration of AST, ALT and alkaline phosphatase enzymes. Serum transaminase levels revert to normal as the hepatic parenchyma heals and hepatocytes regenerate (Thabrew and Joice, 1987). The aqueous extract (Vetriselvan et al., 2011) and ethanolic extract (Sheeja and Kuttan (2006) of Andrographis paniculata lowered the level of alkaline phosphatase which was increased during cyclophosphamide administration, indicated the hepatoprotective effect of the plant extract.

There was no significant difference (P>0.05) in total protein and globulin in group-II, group-III, group-IV, group-V and group-VI; significant (P<0.05) decreased in albumin in group-II, group-V and group-VI but no significant difference (P>0.05) in group-III and group-IV; no significant difference  (P>0.05) in A:G ratio in group-II, group-IV, group-V and group-VI but significant (P<0.05) increased in group-III compared to group-I (Table 3). Olukole et al., (2020) reported that cyclophosphamide caused a significant drop in serum protein levels (albumin, globulin and total protein) compared to control group. Alterations in serum proteins are the clinical markers of assessing the toxicity and health state of humans and animals (Sacher and Mcpherson, 2000). A decrease in total protein and albumin levels implies acute or chronic liver injury, which could be caused by a loss of liver biosynthetic ability (Singh et al., 2011). Hepatoprotective effect of plants is due to presence of flavonoids (Wilma et al., 2011).
The hydro-ethanolic extract of Carica papaya leaves is found better and more hepatoprotective as compared to hydro-ethanolic extract of Andrographis paniculata plant material in quantitative estimation of phytoconstituents and serum biochemical analyses.
None.

  1. Ajanal, M., Gundkalle, M.B., Nayak, S.U. (2018). Estimation of total alkaloid in Chitrakadivati by UV-Spectrophotometer. Ancient Science of Life. 31(4): 198-201.

  2. Akbar, S. (2011). Andrographis paniculata: A review of pharmacological activities and clinical effects. Alternative Medicine Review. 16(1): 66-77.

  3. Alabi, O.A., Harunan, M.T., Anokwuru, C.P., Jegede, T., Abia, H., Okegbe, V. (2012). Comparative studies on antimicrobial properties of extracts of fresh and dried leaves of Carica papaya (L) on clinical bacterial and fungal isolates. Pelagia Research Library. 3(5): 3107-3114. 

  4. Anibijuwon, I.I. and Udeze, A.O. (2009). Antimicrobial activity of papaya on some pathogenic organisms of clinical origin from south-western Nigeria. Ethnobotanical Leaflets. 2009(7): 850-864.

  5. Calzada, F., Yepez-Mulia, L., Tapia-Contreras, A. (2007). Effect of Mexican medicinal plant used to treat trichomoniasis on trichomonas vaginalis. J. Ethnopharmacol. 113(2): 248-251. 

  6. Chandrasekaran, C.V., Thiyagarajan, P., Sundarajan, K. (2009). Evaluation of the genotoxic potential and acute oral toxicity of standardized extract of Andrographis paniculata (KalmCold™). Food and Chemical Toxicology. 47(8): 1892-1902. 

  7. Dwivedi, M.K., Sonter, S., Mishra, S., Patel, D.K., Singh, P.K. (2020). Antioxidant, antibacterial activity and phytochemical characterization of Carica papaya flowers. Beni-Suef University Journal of Basic and Applied Sciences. 9: 23. 

  8. Jiao, Z., Deng, J., Li, G., Zhang, Z., Cai, Z. (2010). Study on the compositional differences between transgenic and non- transgenic papaya (Carica papaya L.). J. Food Comp. Anal. 23: 640-647.

  9. Makkar, H.P.S., Blummel, M., Borowy, N.K., Becker, K. (1993). Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J. Sci. Food Agric. 61: 161-165.

  10. Mir, M.A., Parihar, K., Tabasum, U., Kumari, E. (2016). Estimation of alkaloid, saponin and flavonoid, content in various extracts of Crocus sativa. Journal of Medicinal Plants Studies. 4(5): 171-174.

  11. Mohammed, A., Abubakar, S.A., Sule, M.S. (2011). Hepatoprotective effect of aqueous leaf extract of Carica papaya Linn. against CCl4-induced hepatic damage in rats. International Journal of Pharmaceutical Sciences Review and Research. 11(2): 13-16.

  12. Ojha, S.K., Bharti, S., Joshi, S. et al. (2012). Protective effect of hydroalcoholic extract of Andrographis paniculata on ischaemia-reperfusion induced myocardial injury in rats. Indian J. Med. Res. 135: 414-421. 

  13. Okhuarobo, A., Falodun, J.E., Erharuyi, O., Imieje, V., Falodun, A., Langer, P. (2014). Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: A review of its phytochemistry and pharmacology. Asian Pac. J. Trop Dis. 4(3): 213-222.

  14. Olukole, S.G., Ajayi, T.O., Olaogun, S.C., Ajibola, E.S., Alamu, A.O., Lanipekun, D.O., Egunleti, F.P., Ola-Davies, O.E. (2020). Melatonin Protects Against Cyclophosphamide-Induced Hepatic and Renal Alterations in Rats. Afr. J. Biomed. Res. 23: 407-413.

  15. Ong, H., Chua, S., Milow, P. (2011). Ethno-medicinal plants used by the Temuan villagers in Kampung Jeram Kedah, Negeri Sembilan, Malaysia. Ethno Med. 5: 95-100.

  16. Onyema, C.T., Ofor, C.E., Okudo, V.C., Ogbuagu, A.S. (2016). Phytochemical and Antimicrobial Analysis of Banana Pseudo Stem (Musa acuminata). British Journal of Pharmaceutical Research. 10(1): 1-9. 

  17. Prathanturarug, S., Soonthornchareonnon, N., Chuakul, W., Saralamp, P. (2007). Variation in growth and diterpene lactones among field-cultivated Andrographis paniculata. Journal Natural Medicine. 61: 159-163.

  18. Sacher, R.A. and McPherson, R.A. (2000). Widmann’s clinical interpretation of laboratory tests. FA Davis Company. Washington, D.C. pp.1090.

  19. Saranya, P., Geetha, A., Narmadha, S. (2010). The antioxidant and H+ K+ ATPase inhibitory effect of Andrographis paniculata and andrographolide in vitro and in vivo studies. Pharmacology online. 1: 356-376.

  20. Senthilkumar, S., Devaki, T., Manohar, B.M., Babu, M.S. (2006). Effect of squalene on cyclophosphamide induced toxicity. Clin. Chim. Acta. 364: 335 342. 

  21. Sheeja, K. and Kuttan, G. (2006). Ameliorating Effects of Andrographis Paniculata Extract Against Cyclophosphamide-Induced Toxicity in Mice. Asian Pacific Journal of Cancer Prevention. 7: 609-614.

  22. Singh, A., Bhat, T.K., Sharma, O.P. (2011). Clinical biochemistry of hepatotoxicity. Journal of Clinical Toxicology S:4. https:/ /doi.org/10.4172/2161-0495.S4.

  23. Singha, P.K., Roy, S., Dey, S. (2010). Antimicrobial activity of Andrographis paniculata. Fitoterapia. 74(7-8): 692-694.

  24. Thabrew, M and Joice, P. (1987). A comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Medical. 53: 239-241.

  25. Udoh, P., Essien, I., Udoh, F. (2005). Effect of Carica papaya (paw paw) seeds extract on the morphology of pituitary-gonadal axis of male Wistar rats. Phytother Res. 19(12): 1065-1068.

  26. Unaeze, M.C. and Brikwa, P.O. (1986). Antimicrobial activity of certain medicinal plants used in traditional medicine in Nigeria: Preliminary study. Res. J. Microbiol. 6(2): 32-40. 

  27. Vetriselvan, S., Subasini, U., Rajamanickam C.V., Thirumurugu, S. (2011). Hepatoprotective activity of Andrographis paniculata in ethanol induced hepatotoxicity in albino Wistar rats. Pharmacie Globale International Journal of Comprehensive Pharmacy. 2(2): 1-4.

  28. Wilma, D.S.C., Kavya, N., Kulkarni, S. (2011). Evaluation of insulin sensitivity status in polycystic ovarian syndrome. Asian Pac. J. Trop. Dis. 1(1): 67-70.

  29. Yismaw, G., Tessema, B., Mulu, A., Tiruneh, M. (2008). The in vitro assessment of antibacterial effect of papaya seed extracts against bacterial pathogens isolated from urine, wound and stool. Ethiop. Med. J. 46(1): 71-77.

  30. Zhang, Z., Pan, T., Liu, C., Shan, X., Xu, Z., Hong, H., Lin, H., Chen, J., Sun, H. (2021). Cyclophosphamide induced physiological and biochemical changes in mice with an emphasis on sensitivity analysis. Ecotoxicology and Environmental Safety. 211. 111889.  https://doi.org/10.1016/j.ecoenv.2020. 111889.

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