Effect on Healing Potential of Biosynthesized Silver Nanoparticles in Streptozotocin induce Diabetic Rat Wound Model

DOI: 10.18805/IJAR.B-4832    | Article Id: B-4832 | Page : 704-710
Citation :- Effect on Healing Potential of Biosynthesized Silver Nanoparticles in Streptozotocin induce Diabetic Rat Wound Model.Indian Journal of Animal Research.2022.(56):704-710
Y. Chandnani, M. Roy, C. Sannat, S. Roy, O.P. Mishra drmanjuroy117@gmail.com
Address : Department of Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Anjora, Durg-491 001, Chhattisgarh, India.
Submitted Date : 18-11-2021
Accepted Date : 14-04-2022


Background: Diabetes mellitus is a complex chronic condition that results in hyperglycemic environment affecting the metabolism of proteins and lipids, promotes inflammation and delays wound healing.
Methods: A total of n=30 Wistar rats of either sex were divided into five groups with 6 animals in each. Diabetes mellitus was induced using streptozotocin @ 35 mg/kg body weight and excision wound model was developed in all the animals. Group I and Group II were considered as healthy and diabetic control group respectively. The wounds of Group III, IV and V were treated with topical application of Carica papaya synthesized silver nanoparticles @ 62.5 µg/ml, Carica papaya leafextract @ 31.25 mg/ml and 10% povidone iodine respectively. 
Result: Present study concluded that the characteristics of silver  nanaoparticles synthesized form C. papaya leaf extract make them suitable for the treatment of wound healing in diabetic subjects.


Diabetes Extract Nanoparticles Plant Silver Wound


  1. Annamalai, J. and Nallamuthu, T. (2016). Green synthesis of silver nanoparticles: Characterization and determination of antibacterial potency. Applied Nanoscience. 6: 259-265.
  2. Ashour, A.A., Raafat, D., El-Gowelli, H.M., El-Kamel, A.H. (2015). Green synthesis of silver nanoparticles using cranberry powder aqueous extract: Characterization and antimicrobial properties. International Journal of Nanomedicine. 10: 7207-7221. 
  3. Bitter, T. and Muir, H.M. (1962). A modified uronic acid carbazole reaction. Analytical Biochemistry. 4(4): 330-334.
  4. Chauhan, P.S., Shrivastava, V.I., Prasad, G.B., Tomar, R.S., Shrivastava, V. (2018). Effect of silver nanoparticle- mediated wound therapy on biochemical, hematological and histological parameters. Asian Journal of Pharmaceutical and Clinical Research. 11(3): 251-258.
  5. Deepa, S., Kanimozhi, K., Panneerselvam, A. (2013). Antimicrobial activity of extracellularly synthesized silver nanoparticles from marine derived actinomycetes. International Journal of Current Microbiology and Applied Sciences. 2: 223- 230.
  6. Dische, Z. and Borenfreund, E. (1950). A spectrophotometric method for the microdetermination of hexosamines. The Journal of Biological Chemistry. 184(2): 517-522.
  7. Dwivedi, D., Dwivedi, M., Malviya, S., Singh, V. (2017). Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. Journal of Traditional and Complementary Medicine. 7(1): 79-85.
  8. Elamawi, R.M., Al-Harbi, R.E., Hendi, A.A. (2018). Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi. Egyptian Journal of Biological Pest Control. 28(1): 1-11.
  9. Fagninou, N.A., Tougan, P.U., Nekoua, M., Fachina, R., Koutinhouin, G.B. Yessoufou, A. (2019). Diabetes mellitus: Classification, epidemiology, physiopathology, immunology, risk factors, prevention and nutrition. International Journal of Advanced Research. 7(7): 2320-5407.
  10. Iglay, K., Hannachi, H., Joseph, H.P., Xu, J., Li, X. Engel, S.S. (2016). Prevalence and co-prevalence of comorbidities among patients with type 2 diabetes mellitus. Current Medical Research and Opinion. 32(7): 1243-1252.
  11. Jacob, J.A., Biswas, N., Mukherjee, T., Kapoor, S. (2011). Effect of plant-based phenol derivatives on the formation of Cu and Ag nanoparticles. Colloids and Surfaces B: Biointerfaces. 87(1): 49-53.
  12. Karthik, L., Kumar, G., Kirthi, A. V., Rahuman, A.A., Rao, K.V.B. (2013). Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application. Bioprocess and Biosystems Engineering. 37: 261-267. DOI: 10.1007/s00449-013-0994-3.
  13. Khandel, P., Yadaw, R.K., Soni, D.K., Kanwar, L., Shahi, S.K. (2018). Biogenesis of metal nanoparticles and their pharmacological applications: Present status and application prospects. Journal of Nanostructure in Chemistry. 8: 217-254.
  14. Laden, N.X., Li, D., Stalhe, M. (2016). Transition from inflammation to proliferation: A critical step during wound healing. Cellular and  Molecular Life Sciences. 73(20): 3861-3885.
  15. LeBlanc, L., Pépin, J., Toulouse, K., Ouellette, M.F., Coulombe, M.A., Corriveau, M.P., Alary, M.E. (2006). Fluoroquinolones and risk for methicillin-resistant Staphylococcus aureus, Canada. Emerging Infectious Diseases. 12(9): 1398.
  16. Li, S., Shen, Y., Xie, A., Yu, X., Qiu, L., Zhang, L., Zhang, Q. (2007). Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chemistry. 9(8): 852-858.
  17. Lodhi, S. and Singhai, A.K. (2013). Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn. on streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Medicine. 6(4): 253-9.
  18. Megiel, E. (2017). Surface modification using TEMPO and its derivatives. Advances in Colloid and Interface Science. 250: 158-184. 
  19. Melinamani, D., Prasad, R.V., Lakshmishree, Sundareshan, K.T.S. (2021). Nano Bioscaffolds as Wound Healing Biomaterials in Animals. Indian Journal of Animal Research. Indian Journal of Animal Research. DOI: 10.18805/IJAR.B-4236.
  20. Nayak, B.S., Raju, S.S., Eversley, M., Ramsubhag, A. (2009). Evaluation of wound healing activity of Lantana camara L. - A preclinical study. Phytotherapy Research. 23(2): 241-245.
  21. Neuman, R.E. and Logan, M.A. (1950). The determination of hydroxyproline. The Journal of Biological Chemistry. 184(1): 299-306.
  22. Rai, M., Ingle, A., Gade, A., Duarte, M.C.T., Duran, N. (2015). Synthesis of silver nanoparticles by Phoma gardenia and in vitro evaluation of their efficacy against human disease- causing bacteria and fungi. IET Nanobiotechnology. 9: 71-75.
  23. Shanmugaiah, V., Harikrishnan, H., Al-Harbi, N.S., Shine, K., Khaled, J.M., Balasubramanian, N., Kumar, R.S. (2015). Facile synthesis of silver nanoparticles using Streptomyces sp. VSMGT1014 and their antimicrobial efficiency. Digest Journal of Nanomaterials and Biostructures. 10(1): 179-187.
  24. Singh, V.P., Bali, A., Singh, N., Jaggi, A.S. (2015). Advanced glycation end products and diabetic complications. Korean Journal of Physiology and Pharmacology. 18: 1-14. 
  25. Tavakoli, S. and Klar, A.S. (2020). Advanced hydrogels as wound dressings. Biomolecules. 10: 1169-1182. doi.org/10.3390/biom10081169.
  26. Zhang, Y., Yang, M., Portney, N.G., Cui, D., Budak, G., Ozbay, E., Ozkan, M., Ozkan, C.S. (2008). Zeta potential: A surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells. Biomedical Microdevices. 10(2): 321-328.

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