Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.43

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Full Research Article

Indian Journal of Animal Research, volume 56 issue 5 (may 2022) : 629-632

Molecular Detection and Pathotyping of Newcastle Disease Virus in Pigeons from Karnataka, India

Gopala Lunavat, Basawaraj Awati, K.C. Mallinath, M. Vijay Kumar, Sandeep Halmandge, Arun Kharate, Dasma Bai Banothu, Ashok V. Bhosale, S. Shanmuganathan, K. Kavitha
1Department of Veterinary Microbiology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar-585 401, Karnataka, India.
Cite article:- Lunavat Gopala, Awati Basawaraj, Mallinath K.C., Kumar Vijay M., Halmandge Sandeep, Kharate Arun, Banothu Bai Dasma, Bhosale V. Ashok, Shanmuganathan S., Kavitha K. (2022). Molecular Detection and Pathotyping of Newcastle Disease Virus in Pigeons from Karnataka, India. Indian Journal of Animal Research. 56(5): 629-632. doi: 10.18805/IJAR.B-4824.

ABSTRACT

Background: Newcastle disease (ND) is most important devastating diseases of poultry and it causes huge economic losses to poultry farmers throughout the world. One of the limiting factors in controlling the disease’s spread is the wide host range. While pigeons are highly susceptible and produce neurological signs of the disease. ND was suspected in five dead pigeons and detection of Avian Paramyxovirus-1 (APMV-1) in pigeons (Columba livia domestica) was confirmed by Reverse Transcriptase PCR (RT-PCR). 
Methods: Spleen, lung and trachea were collected and lysates were propagated in allantoic fluids for haemagglutination (HA) by chicken erythrocytes and pigeon convalescent serum inhibited haemagglutination (antibody titers>1/16). 
Result: A 356 bp product was amplified from infected allantoic fluid by targeting a partial Fusion protein gene, including its cleavage site. Pigeon sample isolates were grown in specific pathogen Free (SPF) embryonated chicken eggs (ECE) showed virulent pathotype based on embryonic mean death time (MDT) of 42 hours and the intra-cerebral pathogenicity index (ICPI) in day-old chicks was 1.8. This is the first instance of APMV-1 identification in Karnataka with the possibility of a disease spillover in pigeons. In this scenario, more epidemiological works are suggested for surveillance of NDV in pigeons in this area of work along with implementation of suitable prevention and control measures. 

REFERENCES


  1. Alexander, D.J, Aldous, E.W. and Fuller, C.M. (2012). The long view: A selective review of 40 years of Newcastle disease research. Avian Pathology. 41(4): 329-335.

  2. Cattoli, G., Susta, L., Terregino, C. and Brown, C. (2011). Newcastle disease: A review of field recognition and current methods of laboratory detection. Journal of Veterinary Diagnostic Investigation. 23: 637-656.

  3. Chowdhary, M., Nashiruddullah, N., Roychoudhury, P., Bhat, A., Ahmed, J.A., Kour, K. and Sood, S. (2020). Molecular and virulence characterization of newcastle disease virus in fowls and pigeons from Jammu, India. Indian Journal of Animal Research. 54(10): 1279-1284.

  4. Damena, D., Fusaro, A., Sombo, M., Belainesh, R., Heidari, A. and Kebede, A. (2016). Characterization of Newcastle disease virus isolates obtained from outbreak cases in commercial chickens and wild pigeons in Ethiopia. Springer Plus. 5: 476.

  5. Deepthi, B., Rathnamma, D., Ramani Pushpa, R.N., Shrikanth Isloor, Veeregouda, B.M. and Satyanarayana, M.L. (2020). Molecular and Biological characterization of a Vaccine- Derived Avian Avulavirus type 1 Isolated from an Unvaccinated Goose. Int. J. Curr. Microbiol. App. Sci. 9 (9): 277-285.

  6. Devlin, J.M., Vaz, P.K., Coppo, M.J. and Browning, G.F. (2016).  Impacts of poultry vaccination on viruses of wild birds.  Current Opinion in Virology. 19: 23-29. 

  7. Diel, D.G., Silva, L.H., Liu, H., Wang, Z. and Miller, P.J. (2012). Genetic diversity of an avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infection, Genetics and Evolution. 12: 1770-1779.

  8. Dimitrov, K.M., Ramey, A.M., Qiu, X., Bahl, J. and Afonso, C.L. (2016). Temporal, geographic and host distribution of avian paramyxovirus 1 (Newcastle disease virus). Infection,  Genetics and Evolution. 39: 22-34.

  9. ICTV (2019). Virus Taxonomy: The Classification and Nomenclature of Viruses. The MSL #35th Report of the ICTV.

  10. Kumar, K.A. and Rajan, R.A. (2016). Survey of seroprevalence of Newcastle disease virus in the domestic pigeons of Kanyakumari district (Tamil Nadu), India. International Journal of Veterinary Science. 5(4): 244-249.

  11. Maqbool, R., Wani, S., Wali, A., Kashoo, Z., Dar, P. and Qureshi, S. (2017). RT-PCR based assay for detection of Newcastle disease virus isolated from poultry in Kashmir. Journal of Entomology and Zoology Studies. 5: 1835-1839.

  12. Nanthakumar, T., Tiwari, A.K., Kataria, R.S., Butchaiah, G., Kataria, J.M. and Goswami, P.P. (2000). Sequence analysis of the cleavage site-encoding region of the fusion protein gene of Newcastle disease viruses from India and Nepal.  Avian Pathology. 29(6): 603-607.


  13. OIE (2012). Newcastle disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.3.14.2012; http://www.oie.int/ fileadmin/Home/eng/Health_standards /tahm/2.03.14_NEWCASTLE_DIS.pdf. Accessed on 20 Jan 2017.

  14. Ojasvita, Sanjay, K., Ajit, S., Satbir S., Mahavir, S., Pankaj, K. and Rajendra, Y. (2019). Culture and detection of NDV virus by haemagglutination test (HA) and haemagglutination inhibition test (HI). International Journal of Current Microbiology and Applied Science. 8(09): 474-479.

  15. Roohani, K., Sheau, W.T., Swee, K.Y., Aini, I., Mohd, H.B. and Abdul, R.B.O. (2015). Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with Velogenic NDV.  Journal of Veterinary Science. 16: 447-457.

  16. Roy, P., Venugopalan, A.T. and Koteeswaran, A. (2000). Antigenetically unusual Newcastle disease virus from racing pigeons in India. Tropical Animal Health Production. 32(3): 183-8.

  17. Shan, S., Bruce, K., Stevens, V., Wong, F.Y.K., Wang, J. and Johnson, D.  (2021). In vitro and in vivo characterization of a pigeon paramyxovirus type 1 isolated from domestic pigeons in victoria, Australia. Viruses. 8: 13(3): 429.

  18. Swayne, D.E., Spackman, E. and Pantin-Jackwood, M. (2014). Success factors for avian influenza vaccine use in poultry and potential impact at the wild bird-agricultural interface.  Eco Health. 11: 94-108.

  19. Tirumurugaan, K.G., Kapgate, S., Vinupriya, M.K., Vijayarani, K., Kumanan, K. and Elankumaran, S. (2011). Genotypic and pathotypic characterization of Newcastle disease viruses from India. PloS One. 6(12): 28414.

  20. Toyoda, T., Sakaguchi, T., Hirota, H., Gotoh, B., Kumar, K. and Miyata, T. (1989). Newcastle disease virus evolution II, Lack of gene recombination in generating virulent and avirulent strains. Virology. 169: 273-282. 

  21. Zhan, T., Dongchang, H.L., Xiaolong, L.T. and Wang, W.C. (2021). Biological characterization and evolutionary dynamics of pigeon paramyxovirus type 1 in China. Frontiers in Veterinary Science. 8: 721102.
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)