Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
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Full Research Article
Molecular Detection and Pathotyping of Newcastle Disease Virus in Pigeons from Karnataka, India
First Online 19-04-2022|
Methods: Spleen, lung and trachea were collected and lysates were propagated in allantoic fluids for haemagglutination (HA) by chicken erythrocytes and pigeon convalescent serum inhibited haemagglutination (antibody titers>1/16).
Result: A 356 bp product was amplified from infected allantoic fluid by targeting a partial Fusion protein gene, including its cleavage site. Pigeon sample isolates were grown in specific pathogen Free (SPF) embryonated chicken eggs (ECE) showed virulent pathotype based on embryonic mean death time (MDT) of 42 hours and the intra-cerebral pathogenicity index (ICPI) in day-old chicks was 1.8. This is the first instance of APMV-1 identification in Karnataka with the possibility of a disease spillover in pigeons. In this scenario, more epidemiological works are suggested for surveillance of NDV in pigeons in this area of work along with implementation of suitable prevention and control measures.
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