Gross studies on testes and epididymis of local dog of Assam
The testes of local dog of Assam were reddish white in colour with oval in shape. The testes had of two surface
viz., lateral and medial and two ends
i.
e. upper end and lower end. The lateral surface of testes was convex and the medial surface of testes was flat. The upper end of the testes was occupied by the head of the testes whereas the lower end of the testes was attached to the tail of the epididymis. Mediastinum testis was found in the centre of testes of local dog of Assam. The mediastinum testes were white in colour and well developed in local dog of Assam (Fig 2). The epididymis was consisted of head, body and tail and attached to the testes (Fig 1).
The testes of local dog of Assam were reddish white colour with oval in shape. Similar finding was reported by
Bhagyalakshmi et al., (2020) in dog. The testes had of two surface
viz., lateral and medial and two ends
i.
e. upper end and lower end. The lateral surface of testes was convex and the medial surface of testes was flat. The upper end of the testes was occupied by the head of the testes whereas the lower end of the testes was attached to the tail of the epididymis. Mediastinum testis was found in the centre of testes of local dog of Assam. The mediastinum testes were white in colour and well developed in local dog of Assam. These finding was in accordance with the findings of
Sisson and Grossman (1953). The epididymis was consisted of head, body and tail and attached to the testes. These finding were total agreement with the findings of
Ghosh (2015) in cattle.
Histo-morphological studies on testes of local dog of Assam
Histologically, the testes local dog of Assam were covered by tunica vaginalis, tunica albugenia and tunica vasculosa from outside to inwards (Fig 3). The tunica albugenia was a solid capsule of dense irregular connective tissue. The tunica albuginea was covered by the serous layer
i.
e. the tunica vaginalis. Tunica vasculosa layer was present in the deeper part of the tunica albuginea. Numbers of cross section of blood vessels were observed in the tunica vasculosalayer. The parenchymatous part of the testes was divided into numbers lobules by thick trabeculae or septae which were derived from the tunica albuginea. Numbers of cross-section of blood vessels was found in the septae. The lobules of the testes contained seminiferous tubules,interstitial connective tissue, interstitial cells and blood vessels (Fig 4). Each seminiferous tubule was surrounded by a basement membrane along with smooth muscles. These tubules contained sertoli cells and spermatogenic cells. The spermatogenic cells were spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa (Fig 5). Similar observations were reported by
Mahmud et al., (2015) in testes of one-humped camel bull and
Kishore et al., (2012) in testes of ram. The sertoli cells were attached to the basement membrane of seminiferous tubules. The spermatogonia were very prominent near the basement membrane whereas the spermatids and spermatocytes were present towards the lumen of the seminiferous tubules. The mature spermatids and few spermatozoa occluded the lumen of seminiferous tubules. Interstitial connective tissue and cluster of Leyding cells were found between the semineniferous tubules. The shape of nuclei of leyding cells were large spherical. Apart from the leyding cells, cross-section of blood vessels was foundin interstitial connective tissue. The lining epithelium of epididymis was pseudostratified ciliated columnar epithelium along with basal and columnar cells (Fig 6). The lumens of the epididymis were wide. The cilia of epididymis were stereocilia which help in the movement of spermatozoa (Fig 7). Spermatozoa were also observed in the lumen of epididymis. The epididymides were surrounded by layers of loosely arranged smooth muscles and basement membrane.
Histologically, the testes local dog of Assam was covered by tunica vaginalis, tunica albugenia and tunica vasculosa from outside to inwards. The tunica albugenia was a solid capsule of dense irregular connective tissue. The tunica albuginea was covered by the serous layer
i.
e. the tunica vaginalis. Tunica vasculosa layer was present in the deeper part of the tunica albuginea. Numbers of cross section of blood vessels were observed in the tunica vasculosa layer. The parenchymatous part of the testes was divided into numbers lobules by thick trabeculae or septae which were derived from the tunica albuginea. Numbers of cross-section of blood vessels was found in the septae. These findings were in accordance with the findings of
Moonji and Suwanpugdee (2007) in the testis of Rusa deer. The lobules of the testes seminiferous tubules, interstitial tissue, interstitial cells and blood vessels. Each Seminiferous tubule was surrounded by a basement membrane along with smooth muscles. These tubules contained sertoli cells and spermatogenic cells. The spermatogenic cells were spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. These findings were also supported by
Gofur et al., (2008) in the indigenous bull of Bangladesh. The sertoli cells were attached to the basement membrane of seminiferous tubules. Similar findings were reported by
Mohammed et al., (2011) in the testes of an indigenous male goat. The spermatogonia were very prominent near the basement membrane whereas the spermatids and spermatocytes were present towards the lumen of the seminiferous tubules. The mature spermatids and few spermatozoa occluded the lumen of seminiferous tubules. Interstitial connective tissue and cluster of Leyding cells were found between the semineniferous tubules. The shape of nuclei of Leyding cells were large spherical. Apart from the Leyding cells, cross-section of blood vessels was found in interstitial connective tissue. These findings were in accordance with the findings of
Deka et al., (2014) in testes of Pygmy hog and
Elzoghby et al., (2014) in testes of Sheep. The lining epithelium of epididymis was pseudostratified ciliated columnar epithelium along with basal and columnar cells. The lumens of the epididymis were wide. The cilia of epididymis were stereocilia which help in the movement of spermatozoa. Spermatozoa were also observed in the lumen of epididymis. The epididymides were surrounded by layers of loosely arranged smooth muscles and basement membrane.Similar observations were reported by
Semkov et al., (1984) in testes of calves.