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Indian Journal of Animal Research

Serological Evidence of Avian Metapneumovirus Infection in Layer Chicken Farms of Madhya Pradesh, India

DOI: 10.18805/IJAR.B-4704    | Article Id: B-4704 | Page : 494-497
Citation :- Serological Evidence of Avian Metapneumovirus Infection in Layer Chicken Farms of Madhya Pradesh, India.Indian Journal of Animal Research.2022.(56):494-497
Manoj Kumar, Chakradhar Tosh, S. Nagarajan, Harshad V. Murugkar, Naveen Kumar, Sushil Kumar Tripathi, Pushpendra Kumar Namdeo, Rupal Singh, Suman Mishra, Vijendra Pal Singh manojvet@gmail.com
Address : ICAR-National Institute of High Security Animal Diseases, Anandnagar, Bhopal-462 022, Madhya Pradesh, India.
Submitted Date : 5-07-2021
Accepted Date : 27-10-2021
First Online:

Abstract

Background: Avian metapneumovirus (aMPV) is an important viral agent in chicken and turkey production industry and is most commonly associated with acute upper respiratory tract infection and swollen head syndrome. The disease is of significant economic concern as it is highly contagious and can lead to production losses in chicken and turkey flocks, especially when associated with secondary bacterial pathogens.
Methods: Herein, we have performed a survey on layer farms in selected areas of Madhya Pradesh State, India to ascertain the aMPV status in laying birds, choanal cleft swabs and sera samples were collected for ELISA and RT-PCR assay. A total of 263 sera and 169 choanal cleft swabs from five districts of Madhya Pradesh State (Indore, Bhopal, Sagar, Guna and Bhind) from layer birds of age group 20-72 weeks were screened for aMPV antibodies and for genome. 
Result: Out of these Indore had maximum sero-postivity (73.33%), followed by Sagar (5.00%) and Bhopal (4.08%), respectively. Other two districts did not show any seropostivity against aMPV. RT-PCR assay using published primers and in vitro transcribed RNA of nucleoprotein (N) gene as positive control was carried out in 169 choanal cleft samples from four districts (Indore, Bhopal, Sagar and Guna). All the samples were found negative for aMPV genome by RT-PCR assay. Overall, 26/263 (9.88%) sera were positive to aMPV antibodies. As the chickens in India are not vaccinated against avian metapneumovirus therefore, this study indicates that layers are exposed to this important poultry pathogen. This study warrants further investigation in wider geographical area and isolation of aMPV to design the control strategies for aMPV. 

Keywords

Avian metapneumovirus Chicken Serology

References

  1. Ali, M.Z., Park, J.E., Shin, H.J. (2019). Serological Survey of Avian Metapneumovirus Infection in Chickens in Bangladesh. Journal of Applied Poultry Research. 28: 1330-1334.
  2. Alkhalaf, A.N., Ward, L.A., Dearth, R.N., Saif, Y.M. (2002). Pathogenicity, transmissibility and tissue distribution of avian pneumovirus in turkey poults. Avian Diseases. 46: 650-659.
  3. Bäyon-Auboyer, M.H., Jestin, V., Toquin, D., Cherbonnel, M., Eterradossi, N. (1999). Comparison of F-, G- and N-based RT-PCR protocols with conventional virological procedures for the detection and typing of turkey rhinotracheitis virus. Archives of Virology. 144: 1091-1109.
  4. Buys, S.B., du Preez, J.H., Els, H.J. (1989). The isolation and attenuation of a virus causing rhinotracheitis in turkeys in South Africa. Onderstepoort Journal of Veterinary Research. 56: 87-98.
  5. Cook, J.K.A., Cavanagh, D. (2002). Detection and differentiation of avian pneumoviruses (Metapneumoviruses). Avian Pathology. 31: 117-132.
  6. Cook, J.K.A., Chesher, J., Orthel, F., Woods, M.A., Orbell, S.J., Baxendale, W., Huggins, M.B. (2000). Avian pneumovirus infection of laying hens: experimental studies. Avian Pathology. 29: 545-556.
  7. Eswaran, M.A., Sukumar, M., Johnson, K., Rajeswar, J., Balasubramaniam, G.A., Anna, T. (2014). Serological survey of avian metapneumo virus infection in broiler breeder chicken farms in Tamil Nadu. International Journal of Advanced Veterinary Science and Technology. 3(1):  84-87.
  8. Gough, R.E. (2003). Avian Pneumoviruses. In: Diseases of Poultry, Eleventh edition, [Saif, Y.M, Barnes, H.J., Glisson, J.R., Fadly, A.M., McDougald, L.R., Swayne, D., (eds)]. Blackwell Publishing. 92-99.
  9. Gough, R.E., Manvell, R.J., Drury, S.E., Pearson, D.B. (1994). Isolationof an avian pneumovirus from broiler chickens. Veterinary Record. 134: 353-354.
  10. Jones, R.C. (2010). Viral respiratory diseases (ILT, aMPV infections, IB): are they everunder control? British Poultry Science. 51: 1-11.
  11. Jones, R.C., Naylor,C. J., Bradbury, J.M., Savage, C.E., Worthington, K., Williams, R.A. (1991). Isolation of a turkey rhinotracheitis -like virus from broiler breeder chickens in England. Veterinary Record. 129: 509-510.
  12. Jones, R.C., Rautenschlein, S. (2013). Avian Metapneumovirus. In: Diseases of Poultry. [Swayne, D.E. (Ed.)], Ames, IA, Wiley-Blackwell. 112-138.
  13. Kwon, J.S., Lee, H.J., Jeong, S.H., Park, J.Y. Hong, Y.H. et al. (2010). Isolationand characterization of avian metapneumovirus from chickens in Korea. Journal of Veterinary Science. 11: 59-66.
  14. OIE (2016): Chapter 2. 3. 15: Turkey Rhinotracheitis.
  15. Owoade, A.A., Ducatez, M.F., Hubschen, J.M., Sausy, A., Chen, H., Guan, Y. and Muller, C.P. (2008). Avian metapneumovirus subtype A in China and subtypes A and B in Nigeria.Avian Diseases. 52: 502-506.
  16. Park, J.E., Lee, D.W., Shin, H.J. (2011). Serological survey of antibodies against avian metapneumovirus in Korean chicken flocks. Journal of Applied Poultry Research. 20: 573-576.
  17. Pedersen, J.C., Senne, D.A., Panigrahy, B., Reynolds, D.L. (2001). Detection of avian pneumovirus in tissue and swab specimens from infected turkeys. Avian Diseases. 45: 581-592.
  18. Sugiyama, M., Koimaru, H., Shiba, M., Ono, E., Nagata, T., Ito, T. (2006). Drop of egg production in chickens by experimental infection with an avian metapneumovirus strain PLE8T1 derived from swollen head syndrome and the application to evaluate vaccine. Journal of Veterinary Medical Science. 68: 783-787.
  19. Surajit, B., Nirav, R., Pravinsinh, C. (2018).  Seroprevalence of Avian Pneumovirus in broiler breeders in India. Indian Journal of Poultry Science. 53: 120-122.
  20. Velayudhan, B.T., Mccomb, B., Bennett, R.S., Lopes, V.C., Shaw, D., Halvorson, D.A., Nagaraja, K.V. (2005). Emergence of a virulent type C avian metapneumovirus in turkeys in Minnesota. Avian Diseases. 49: 520-526.

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