Protective role and antioxidant activity of arabic gum against trichloroacetate-induced toxicity in liver of male rats

DOI: 10.18805/ijar.10976    | Article Id: B-466 | Page : 303-309
Citation :- Protective role and antioxidant activity of arabic gum againsttrichloroacetate-induced toxicity in liver of male rats .Indian Journal Of Animal Research.2017.(51):303-309

Najla O. Ayaz, Kholoud S. Ramadan*, Hoda E.A. Farid and Hanan S. Alnahdi

ramadankholoud@yahoo.com
Address :

Department of Biochemistry, Faculty of Science-AlFaisaliah, King Abdulaziz University Jeddah, Saudi Arabia. 

Submitted Date : 22-02-2016
Accepted Date : 19-05-2016

Abstract

Investigation of functional hepatoprotective components from plants origin is a new model for drug evolution. The study examined the protective effects of Arabic gum (AG) induction on liver serum markers, antioxidant enzymes and lipid peroxidation in liver tissue against trichloroacetate (TCA) induced hepatotoxicity in male albino rats. Also, the chemical constituents and antioxidant assay of aqueous extract of AG was determined. TCA at the dose of 50 mg/kg for 2 months produced hepatotoxicity as examined by the significant increase of serum activities of ALT, AST, ALP and conjugated bilirubin level in the TCA treated animals alone and decrease in total protein and albumin levels. Pretreatment with AG aqueous extract (0.5 g/kg/day) significantly (P< 0.05) lowered the serum enzyme activities and increased in total proteins and albumin level. The antioxidant studies showed that the activities of hepatic SOD, CAT and GPx were decreased in TCA induced animals with significantly elevated in MDA content which is reversed in pretreatment with aqueous extract of AG. Histopathology of the liver sections confirmed that the AG extract ameliorated hepatic damage induced by TCA. In conclusion, the study demonstrated that AG supplementation for 2 months in TCA induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA. 

Keywords

Antioxidants Arabic gum Biochemical parameters Histopathology Lipid peroxidation Trichloroacetate.

References

  1. Acgih, (1991). Documentation of the Threshold Limits Values and Biological Exposure Indices, 6th ed. Cincinnati, OH: American Conference of Governmental Industrial Hygienists.
  2. Acharya, S., Mehta, K., Rodriguez, S., Pereira, J., Krishnan, S. and Rao, C.V. (1997). A histopathological study of liver and kidney in maleWistar rats treated with subtoxic doses of t-butyl alcohol and trichloroacetic acid. ExpToxicol Pathol., 49:369–373.
  3. AL-Farsi, M., Alasalva, C., Morris, A., Baron, M. and Shahidi, F. (2005). Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties growth in Oman. J Agric Food Chem., 53:7592–7599.
  4. Ames, B.N., Shigenaga, M.K. and Hagen, T.M. (1993). Oxidants, antioxidants and the generative disease of aging. Proceedings of the National Academy of Sciences of the United States of America, 90: 7915-7922. 
  5. Bartold, P. M., Wiebkin, O. W., and Thonard, J. C. (1984). The effect of oxygen-derived free radicals on gingival proteoglycans and hyaluronic acid. Journal of Periodontology, 19: 390-400. 
  6. Brattin, W.J., Glende, J.E.A and Recknagel, R.O. (1985) Pathological mechanisms in carbon tetrachloride hepatotoxicity. J Free Radic Biol Med., 1:27–38.
  7. Carleton, H. (1979). Histological Techniques. 4th Edn. Oxford University Press, London, New York, USA.
  8. Celik, I. (2007) Determination of toxicity of trichloroacetic acid in rats: 50 days drinking water study. Pestic Biochem Phys., 89: 39–45.
  9. Cheng, Z., Li, Y. and Chang, W. (2003). Kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a Fenton-type reaction system. Anal. Chim. Acta., 478: 129-137.
  10. Daniel, F.B., DeAngelo, A.B., Stober, J.A., Olson, G.R. and Page, N.P. (1992). Hepatocarcinogenicity of chloral hydrate, 2- chloroacetaldehyde, and dichloroacetic acid in the male B6C3F1 mouse. Fund Appl Toxicol., 19:159–168.
  11. Gamal el-din, A.M., Mostafa, A.M., Al-Shabanah, O.A., Al-Bekairi, A.M. and Nagi, M.N. (2003). Protective effect of arabic gum against acetaminophen-induced hepatotoxicity in mice. Pharmacological Research, 48: 631-635.
  12. Halliwell, B., and Gutteridge, J. M. (1984). Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet, 23: 1396-1397. 
  13. Hooda, M. S., Rishi Pal, A. and Bhandari, J. S. (2013). Antidiabetic activity of Acacia senegal pod extract in streptozotocininduced diabetic rats. Int. J. of Indigenous Medicinal Plants, 46: 1400-1404.
  14. James, L. A. and Webb, Jr. (1985). The Trade in Gum Arabic: Prelude to French Conquest in Senegal. Journal of African history, 26: 49-168
  15. Julkunen-Titto, R. (1985). Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J. Agric. Food Chem., 33: 213–217.
  16. Kassem, A. (2015). Dietary gum arabic supplementation alter plasma and tissue antioxidant and free radical scavenging activities in Sprague Dawley Male Rats. Journal of Biology and Life Science, 6: 129-138.
  17. Kaur, C. and Kapoor, H.C. (2001). Antioxidant in fruits and vegetables—the millennium’s health. Int J Food Sci Technol., 36:703–25.
  18. Kind, P.R.N. and King, E.J. (1954). Determination of Serum Alkaline Phosphatase. Journal of Clinical Pathology, 7: 132-136.
  19. Liyana-Pathirana, C.M and Shahidi, F. (2005). Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chem., 93:47–56. 
  20. Lotito, S.B. and Frei, B. (2004).The increase in human plasma antioxidant capacity after apple consumption is due to the metabolic effect of fructose on urate, not apple-derived antioxidant flavonoids. Free Radical Biology and Medicine, 37: 251-258.
  21. Malloy, H.T. and Evelyn, K.A. (1937). The determination of bilurubin with the photoelectric colorimeter. Journal of Biological Chemistry, 119: 481-485.
  22. Negri, G., Teixeira, E.W., Alves, M.L., Moreti, A.C., Otsuk, I.P., Borguini, R.G. and Salatino, A. (2011) Hydroxycinnamic acid amide derivatives, phenolic compounds and antioxidant activities of extracts of pollen samples from Southeast Brazil. J Agric Food Chem., 59:5516–5522
  23. Pang, S., Xin, X. and Stpierre, M.V. (1992). Determinants of metabolic disposition. Ann Rev Pharmacol Toxicol., 32: 625–626. 
  24. Panuganti, S.D., Khan, FD. and Svensson, C.K. (2006). Enhanced xenobiotic-induced hepatotoxicity and kupffer cell activation by restraint-induced stress. J Pharmacol Exp Ther., 318: 26–34.
  25. Rajesh, M.G. and Latha, M.S. (2004). Preliminary evaluations of the antihepatotoxic effect of Kamilari, a polyherbal formulation. J Ethnopharmacol., 91:99–104
  26. Rehman, K. U., Codipilly, C. N. and Wapnir, R. A. (2004). Modulation of small intestinal nitric oxide synthase by gum Arabic. Exp Biol Med., 229: 895-901.
  27. Reitman, S. and Frankel, S (1957). Glutamic – pyruvate transaminase assay by colorimetric method. Am. J. Clin. Path., 28: 56.
  28. Richardson, S.D., Thruston, A.D., Jr., Krasner, S.W., Weinberg, H.S., Miltner, R.J., Schenck, K.M., Narotsky, M.G., McKague, A.B. and Simmons, J.E. (2008). Integrated Disinfection byproducts mixtures research: Comprehensive characterization of water concentrates prepared from chlorinated and ozonated/postchlorinated drinking water. J. Toxicol. Environ. Health A., 71:1165–1186
  29. Slater, T. F. (1984). Free-radical mechanisms in tissue injury. Biochemical Journal, 222: 1-15.
  30. Treitz, N.W. (1970). Fundamentals of Clinical Chemistry with Clinical Correlation. W.B. Sanders, Philadelphia. pp. 280–284.
  31. Varani, J., Fligiel, S. E. G. ., Till, G. O., Kunkel, R. G. , Ryan, V. S., and Ward, P. A.(1985). Pulmonary endothelial cell killing by human neutrophils: possible involvement of hydroxyl radical. Laboratory Investigation, 656-663.
  32. Verbeken, et al., (2003). Dewettinck Exudate gums: occurrence, production, and application. Applied Microbiology and Biotechnology, 63: 10-21.
  33. Ward, F.M. and Daly, M.J. (1999). Hepatic disease. In. Clinical Pharmacy and Therapeutics. [Walker R, Edward C, Eds], Churchill Livingston, New York :195-212. 
  34. Zhang, L., Ravipati, A.S., Koyyalamudi, S.R, Jeong, S.C., Reddy, N., Smith, P.T, Bartlett J., Shanmugam, K., Münch, G. and Wu, M.J. (2011). Antioxidant and anti-inflammatory activities of selected medicinal plants containing Phenolic and Flavonoids Compounds. Journal of Agriculture and Food Chemistry, 59: 12361-12367.
  35. Zhishen, J., Mengcheng, T. and Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559.
  36. Zimmermann, M. (1983) Ethical guidelines for investigations of experimental pain in conscious animals. Pain, 16:109–110.

Global Footprints