Comparison of LFCI, LAT and ELISA for the diagnosis of toxoplasmosis in goats 

DOI: 10.18805/ijar.9593    | Article Id: B-464

Sadaf Niaz, Rafi Ullah*, Bahrawar Said, Sumaira Shams, Naser M. AbdEl-Salam and Riaz Ullah
Address :

Department of Zoology, Abdul Wali Khan University, Mardan (KPK) Pakistan.

Submitted Date : 16-02-2016
Accepted Date : 8-03-2016


This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T .gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii.  The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.


ELISA LAT LFCI Toxoplasma gondii.


  1. Abu-Dalbou, M.A., Ababneh., M.M, Giadinis., N.D and Lafi., S.Q (2010). Ovine and Caprine Toxoplasmosis. Iranian Journal of Veterinary Science and Technology, 2: 61-76.
  2. Ahmad, N., Iqbal, Z., Mukhtar, M., Mushtaq, M., Khan, K.M. and Qayyum, M., (2015). Seroprevalence and Associated Risk Factors of Toxoplasmosis in Sheep and Goats in Pothwar Region, Northern Punjab Pakistan. Pakistan Journal of Zoology, 47: 161-167.
  3. Alvarado-Esquivel, C., García-Machado, C., Vitela-Corrales, J., Villena, I. and Dubey, JP., (2011). Seroprevalence of Toxoplasma gondii infection in domestic goats in Durango State Mexico, Veterinary Parasitology, 183: 43-6.
  4. Chandrawathani, P., Nuralaini, R., Zanin, C.M., Premaalatha B., Adnan, M, Jamnah, O. and Khor, S.K., (2008). Seroprevalenceof Toxoplasmagondii in pigs, goats, cattle, dogs and cats in Peninsular, Malaysia. Tropical Biomedicine, 25: 257-258.
  5. Dubey, J., (2008). The history of Toxoplasma gondii-the first 100 years. Journal of Eukaryotic Microbiology, 55: 467-475.
  6. Ertug, S., Okyay, P., Turkmen, M. and Yuksel, H., (2005). Seroprevalence and risk factors for Toxoplasma infection among pregnant women in Aydin province, Turkey. Biomedical central Public Health, 5: 2458-2466.
  7. Etheredge, G.G., Michael, G., Muehlenbein, M.P. and Frenkel, J.K., (2004). The role of cats and dogs in the transmission of Toxoplasma infection in Kuna and Ember a children in eastern Panama. American Journal of Public Health, 16: 176-186.
  8. Iovu, A., Gyorke, A., Mircean, V., Gavrea, R. and Cozma V., (2012). Seroprevalence of Toxoplasma gondii and Neosporacaninum in dairy goats from Romania. Veterinary Parasitology, 186:470-4.
  9. Jittapalapong, S., Sangvaranond, A., Pinyopanuwat, N., Chimnoi, W., Khachaeram, W., Koizumi, S., and Maruyama, S. (2005). Seroprevalence of Toxoplasma gondii infection in domestic goats in Satun Province, Thailand, Veterinary Parasitology, 127: 17-22.
  10. Kamani, J., Mani, A.U. and Egwu, G.O., (2010). Seroprevalence of Toxoplasma gondii infection in domestic sheep and goats in Borno state, Nigeria. Tropical Animal Health Production, 42: 793-7.
  11. Lashari, M.H. and Tasawar, Z., (2010). Seroprevalence of Toxoplasmosis in Sheep in Southern Punjab, Pakistan. Pakistan Veterinary Journal,30 pp 91-94.
  12. Neto, JO, Azevedo, SS, Gennari, SM, Funada, MR, Pena, HF, Araújo, AR, Batista, CS, Silva, ML, Gomes, AA, Piatti, RM and Alves, CJ (2008). Prevalence and risk factors for anti-Toxoplasma gondii antibodies in goats of the Seridó Oriental microregion, Rio Grande do Norte state, Northeast region of Brazil, Veterinary Parasitology, 156:329-32.
  13. Radostits, O.M., Blood, D.C. and Gay, C.C., (1994). A text book of disease of cattle, sheep, pigs, goats and horses. 8th ed, W. B, Saunders, London: 1201-1206. 
  14. Ramzan, M., Akhtar, M., Muhammad, F., Hussain, I., Hiszczyñska-Sawicka, E., Haq, A.U., Mahmood, M.S. and Hafeez, M.A., (2009). Seroprevalence of Toxoplasma gondii in sheep and goats in Rahim Yar Khan (Punjab), Pakistan. Tropical Animal Health Production, 41: 1225-9.
  15. Shah, M., Zahid, M., Asmat, P., Alam, A. and Kausar, A., (2013). Seroprevalence of Toxoplasma gondii infection in goats and sheep of district Mardan, Pakistan. International Journal of Bioscience, 3 :1-8.

Global Footprints