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Artemia salina lethality and histopathological studies on Bacopa monnieri leaf extract

DOI: 10.18805/ijar.v0iOF.7606    | Article Id: B-462 | Page : 610-614
Citation :- Artemia salina lethality and histopathological studies onBacopa monnieri leaf extract .Indian Journal of Animal Research.2018.(52):610-614

Natthawut Charoenphon, Niwat Kangwanrangsan and Wannee Jiraungkoorskul

pathobiologymu@gmail.com
Address :

Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Submitted Date : 15-02-2016
Accepted Date : 10-11-2016

Abstract

Bacopa monnieri leaf aqueous and methanolic extractions at 1, 3, 5 and 24 hours were determined for the highest amount of total phenolic compound and used for evaluating the toxicity test against Artemia salina at varied concentrations as 0, 5, 50, 100, 500, 1000, 2,500 and 5,000 ppm, by determining the median and 90% lethal concentration, LC50 and LC90, respectively, within 24 hours. The result revealed that the total phenolic compound measurements in 1, 3, 5 and 24 hours were 70.58+2.74, 70.52+1.63, 67.11+2.51 and 79.01+2.87 mg of gallic acid equivalent per gram of aqueous extractions, and 37.88+1.57, 43.08+2.74, 45.86+2.68 and 55.31+3.60 mg of gallic acid equivalent per gram of methanolic extractions, respectively. Due to the highest amount of total phenolic compound, the 24-hour aqueous extract of B. monnieri leaf expressed the 24-h LC50 and LC90 values in A. salina and they were 3,577.90 and 6,440.22 ppm, respectively. Under histological analysis, pathological lesions like cellular swelling, and elongation of the epithelial cells and edema were observed. More additional, cells protruding into the lumen and absence of microvilli were also found. 

Keywords

Artemia salina Brine shrimp Bacopa monnieri Histopathology Leave Total phenolic compound.

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