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Optimization of Enzymatic Extraction of ACE Inhibitory Peptide from Rohu (Labeo rohita) Fish Waste using RSM

DOI: 10.18805/IJAR.B-4542    | Article Id: B-4542 | Page : 673-679
Citation :- Optimization of Enzymatic Extraction of ACE Inhibitory Peptide from Rohu (Labeo rohita) Fish Waste using RSM.Indian Journal of Animal Research.2022.(56):673-679
Vikas Kumar, R. Jeya Shakila, A.U. Muzaddadi, G. Jeyasekaran, D. Sukumar, P. Padmavathy, Yogesh Kumar vikas.kumar5@icar.gov.in
Address : ICAR-Central Institute of Post-Harvest Engineering and Technology, Ludhiana-141 004, Punjab, India.
Submitted Date : 25-05-2021
Accepted Date : 27-07-2021

Abstract

Background: Hypertension is one of the cardiovascular disease that kills people silently across the globe. It can be controlled, in one of the way, by ACE inhibitory peptide extracted from aquatic resources. 
Methods: Rohu (Labeo rohita) fish wastes were quantified for their anatomical yield; analyzed for their proximate composition and optimized the enzymatic extraction parameters for ACE inhibitory peptides. Response surface methodology with Box-Benhken Design (RSM-BBD) was used to optimize alcalase concentration (0.5-2% v/w), hydrolysis temperature (45-60°C), hydrolysis time (60-240 min.) and solid: liquid (S/L) ratio (0.2-1) to obtain rohu fish waste peptides. 
Results: More waste generated in smaller (49.4%) than medium and bigger (34.5%) fish. Quantum of edible flesh (59.06%) was followed by head (23.9%), trimmings (5.18%), scales (4.19%) and swim bladder (0.65%). However, protein content was highest in swim bladder (34.1%) followed by scales (22.9%), trimmings (18.7%) and head (17.1%). Alcalase concentration (1.08%, v/w), temperature (52.10°C), hydrolysis time (129.18 min) and S/L ratio (0.8:1) were found optimum for extraction ACE inhibitory peptide with DH, ACE inhibition and PY of 19.27%, 54.98% and 51.37% respectively. Results showed the potential of extracted ACE inhibitory peptide as ingredients in functional food.

Keywords

ACE inhibitory peptide Alcalase Anatomical yield Enzymatic ecxtraction Optimization Proximate composition Rohu fish waste

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