Pharmacological Evaluation of Antipyretic, Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Cassia fistula

DOI: 10.18805/IJAR.B-4531    | Article Id: B-4531 | Page : 483-488
Citation :- Pharmacological Evaluation of Antipyretic, Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Cassia fistula.Indian Journal of Animal Research.2022.(56):483-488
D.K. Sharma, S.K. Sharma, M.K. Lonare, Rajdeep Kaur, V.K. Dumka dineshcovas79@gmail.com
Address : Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
Submitted Date : 19-05-2021
Accepted Date : 27-07-2021


Background: The antipyretic, analgesic and anti-inflammatory activities of two concentrations (100 and 200 mg/kg) of ethanolic extract of leaf, bark, flower and fruit pulp of C. fistula were determined in male wistar albino rats. 
Methods: Antipyretic activity was assessed by E. coli endotoxin induced pyrexia. Analgesic activity was assessed by hot plate, tail immersion and acetic acid induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced rat paw edema assay. 
Result: Significant (p<0.05) antipyretic activity was exhibited from 2h onwards by bark extract @ 200 mg/kg and from 3h onwards by bark extract @100 mg/kg and leaves extract @ 200mg/kg as compared to control group. Significant (p<0.05) analgesic activity was shown by extract of bark @ 200 mg/kg as it is evident by increase in reflex time in hot plate (90,120,180 min), tail immersion test (120,180 min) and inhibition of writhing (32.12%). Significant (p<0.05) anti-inflammatory activity was exhibited from 3h post administration by bark @ 200 and leaves @ 100 and 200 mg/kg.


​Analgesic Anti-inflammatory Antipyretic Cassia fistula


  1. Ajay, Kumar, K., Satish, S., Ibrahhim, S. and Hedge, K. (2017). Therapeutic Uses of Cassia fistula: Review International Journal of Pharmacology and Chemical Research. 3(1): 38-43.
  2. Ali, M.A., Sagar, H.A., Khatun, M.C.S., Azad, A.K., Begum, K. and Wahed, M.I.I. (2012). Antihyperglycemic and analgesic activities of ethanolic extract of Cassia fistula (L.) stem bark. International Journal of Pharmaceutical Sciences and Research. 3(2): 416-423.
  3. Bhakta, T., Mukherjee, P.K., Saha, K., Pal, M., Saha, B.P. and Mandal, S.C. (1999). Evaluation of anti-inflammatory effects of Cassia fistula (Leguminosae) leaf extract on rats. Journal of Herbs, Spices and Medicinal Plants. 6(4): 67-72.
  4. Das, S., Sarma, G. and Barman, S. (2008). Hepatoprotective activity of aqueous extract of fruit pulp of Cassia fistula (AFCF) against carbon tetrachloride (CCL4) induced liver damage in albino rats. Journal of Clinical and Diagnostic Research. 2: 1133-1138.
  5. Dogan, M.D., Patel, S., Rudaya, A.Y., Steiner, A.A., Szekely, M. and Romanovsky, A.A. (2004). Lipopolysaccharide fever is initiated via a capsaicin sensitive mechanism independent of the subtype 1 vanilloid receptor. British journal of Pharmacology, 143(8): 1023-1032.
  6. Eddy, N.B. and Leimbach, D. (1953). Synthetic analgesics. II. Dithienylbutenyl-and dithienylbutylamines. Journal of Pharmacology and Experimental Therapeutics. 107(3): 385-393.
  7. Fontenele, J.B., Viana, G.S., Xavier-Filho, J. and De-Alencar, J.W. (1996). Anti-inflammatory and analgesic activity of a water-soluble fraction from shark cartilage. Brazilian journal of Medical and Biological Research= Revista Brasileira de Pesquisas Medicas e Biologicas. 29(5): 643.
  8. Gobianand, K., Vivekanandan, P., Pradeep, K., Mohan, C.V.R. and Karthikeyan, S. (2010). Anti-inflammatory and antipyretic activities of Indian medicinal plant Cassia fistula Linn. (Golden Shower) in Wistar albino rats. International Journal of Pharmacology. 6(6): 719-725.
  9. Janssen, P.A.J. (1963). The inhibitory effect of fentanyl and other morphine-like analgesics on the warm water induced tactile withdrawal reflex in rat. Arzneimittelforschung.13: 502-507.
  10. Karbab, A., Mokhnache, K., Ouhida, S., Charef, N., Djabi, F., Arrar, L. and Mubarak, M.S. (2020). Anti-inflammatory, analgesic activity and toxicity of Pituranthos scoparius stem extract: An ethnopharmacological study in rat and mouse models. Journal of Ethnopharmacology. 112936.
  11. Nair, B., Punniamurthy, N. and Kumar, S.K. (2017). Ethno-veterinary practices for animal health and the associated Medicinal Plants from 24 Locations in 10 States of India. RRJVS Journal of Veterinary Science. 3(1): 25-34.
  12. Panda, S. K., Padhi, L. P. and Mohanty, G. (2011). Antibacterial activities and phytochemical analysis of Cassia fistula (Linn.) leaf. Journal of Advanced Pharmaceutical Technology and Research. 2(1): 62-67.
  13. Patwardhan, S., Sakhare, G., Singhai, A.K., Jain, K. and Somani, R. (2009). Evaluation of analgesic activity of Cassia fistula on albino mice. Pharmacologyonline. 2: 887-893.
  14. Pawar, A.V. and Killedar, S.G. (2017). Uses of Cassia fistula Linn. as a medicinal plant. International Journal for Advance Research and Development. 2(3): 85-91.
  15. Rahmani, A.H. (2015). Cassia fistula Linn: Potential candidate in the health management. Pharmacognosy Research. 7(3): 217-224.
  16. Rajeswari, R., Thejomoorthy, P., Mathuram, L.N. and Narayana Raju, K.V.S. (2006). Anti-inflammatory activity of Cassia fistula Linn. bark extracts in sub-acute models of inflammation in rats. Tamilnadu Journal of Veterinary and Animal Sceicnes.  2(5): 193-199.
  17. Rastogi, S., Pandey, M.K., Prakash, J., Sharma, A. and Singh, G.N. (2015). Veterinary herbal medicines in India. Pharmacognosy Reviews. 9(18): 155-163.
  18. Ratnasooriya, W.D. and Dharmasiri, M.G. (1999). A water extract of leaves and stems of Psychotria sarmentosa has analgesic and antihyperalgesic activity in rats. Medical Science Research. 27: 715-718.
  19. Sahoo, N., Manchikanti, P. and Dey, S. (2010). Herbal drugs: Standards and regulation. Fitoterapia. 81(6): 462-471.
  20. Silva, G.N., Martins, F.R., Matheus, M.E., Leitao, S.G. and Fernandes, P.D. (2005). Investigation of anti-inflammatory and antinociceptive activities of Lantana trifolia. Journal of Ethnopharmacology. 100(3): 254-259.
  21. Tikole, S.S., Bagde, P., Powar, P. and Shelar, P. (2013). Study of the anti-inflammatory activity of Cassia Fistula Linn. Journal of Current Pharma Research. 3(3): 918.
  22. Winter, C.A., Risley, E.A. and Nuss, G.W. (1962). Carrageenin- induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine. 111(3): 544-54.

Global Footprints