Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 56 issue 4 (april 2022) : 412-415

A Study on Oestrus and Parturition Behaviour of Manipuri Pony Mares

Ningthoukhongjam Linda1,*, Fazal Ali Ahmed1, Thingujam Chaa Mangilal2
1Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl-796 014, Mizoram, India.
2State Veterinary Department of Manipur, Lamphelpat-795 001, Manipur, India.
Cite article:- Linda Ningthoukhongjam, Ahmed Ali Fazal, Mangilal Chaa Thingujam (2022). A Study on Oestrus and Parturition Behaviour of Manipuri Pony Mares . Indian Journal of Animal Research. 56(4): 412-415. doi: 10.18805/IJAR.B-4529.
Background: The aim of this research was to study the behavioural signs exhibited during the different phases of oestrus and parturition in Manipuri pony mares.

Methods: A total of ten each for oestrus and pregnant female Manipuri Ponies were selected for the behavioural signs study and a total data of 30 numbers of set questionnaires were analysed for studying the oestrus and foaling during various seasons and months of Manipuri Pony mares.

Result: The behavioural oestrus signs observed in female Manipuri pony were raised tail, vulva swelling, vaginal mucous secretion, stallion seeking, inappetance and male acceptance. Pre-partum sign includes swelling of vulva, waxing, udder development, teats engorgement with milk discharges. Restlessness, isolation from human and herd mates, abdominal straining were shown during first stage of parturition. Abdominal straining with expulsion of fetus was displayed in the second stage followed placenta expulsion at the third stage of parturition. The occurrences of timing of birth were lesser between 6 pm and midnight (20.00%). The highest occurrence of oestrus was recorded during spring season (46.67%, 56.67%) respectively and in months it occurred highest during March to June (16.67%).
Manipuri pony (Equus ferus caballus) is an important registered equine breed of India under registration number (INDIA _ HORSE _ 1200 _ MANIPURI _ 07003) which has been breeding in the erstwhile kingdom of Manipur, India over centuries.
       
There was a negative growth of -35.66% (percentages of deviation) during 2003 to 2007 of the pony population in its breeding state and National Bureau of Animal Genetic Resources considered Manipuri pony as threatened breed which is due to the increasing pressure of urbanization their habitation have been forfeited by the encroaching human hordes. The total number of ponies recorded in 19th livestock census 2012 was 1042 in rural areas and 59 figures in urban areas.
       
For preserving the genetic heritage of Manipuri pony populations, it is essential to characterize their reproductive traits and functions and much needed to learn about the features of females if breeding and conservation programmes are to be successful in the near future but reliable data on reproduction has neither been identified nor has any objective study been made on reproductive performance. Consequently, the present study was undertaken keeping the prime objective of studying the behavioural signs of oestrus and parturition of Manipuri pony mares.
Manipur Horse Riding and Polo Association, Lamphelpat, Imphal West district of Manipur, India was selected as study area keeping in view of the confined population of Manipuri ponies in the area (24.30°N - 25.00°N latitudes and 93.45°E - 94.15°E longitudes). The research protocol was permitted by the College of Veterinary Sciences and Animal Husbandry, CAU, Mizoram, India. The study was undertaken systematically for a period of seventeen months i.e. from January, 2018 to June, 2019.
 
Animals
 
All the experimental ponies were maintained under standard feeding and managemental conditions. The mares at this farm were bred by natural mating. A total of ten pregnant (due term) and 10 oestrus female Manipuri ponies were selected for the present study.
 
Oestrus behaviour study
 
All the mares were observed twice daily from 6 to 7 am in morning and from 5 to 6 pm in the evening. Female Manipuri ponies were confirmed for oestrus by using a teaser stallion followed by rectal palpation as per Talluri et al., (2016). The oestrus symptoms of all mares were carefully observed and behavioural signs were studied and recorded.
 
Parturition behaviour study
 
The pre-partum physical signs were observed before the expected day of parturition till the time of foaling.
       
Foaling signs were recorded as per Blanchard et al., (2003). All the animals were kept confined during this period and constant observations were made throughout the period (a day before expected date of foaling to the time of expulsion of placenta). The different stages of parturition were observed and recorded. The onset of second stage of labour was considered with the appearance of the amniotic bag and the second stage was observed and recorded till the foal is completely delivered followed the placental expulsion. Placenta expulsion was recorded as the time between expulsions of the foal till expulsion of placenta.
       
The timing of the birth was recorded as per Meliani et al., (2013).
 
Occurrence of oestrus in Manipuri Pony during various seasons
 
It was determined from the structured questionnaires collected from the Central Agricultural University and Manipur Horse Riding and Polo Association including individual pony owners. A total data of 30 numbers of such questionnaires were collected and analysed.
 
Statistical analysis
 
The data were subjected for statistical analysis by means of descriptive analysis (Micro Soft Excel-2010; http://office. microsoft.com).
Oestrus behaviour study
 
The frequencies of occurrence of different behavioural signs of oestrus in female Manipuri pony are shown in (Table 1). In the present study the behavioural oestrus signs observed in female Manipuri pony was raised tail, vulva swelling, vaginal mucous secretion, stallion seeking, inappetance and male acceptance were found to be analogous with the observations of (Emady,1999; Taberner et al., 2008 and Panwar and Yadav, 2010). Although winking of clitoris has been described as an outstanding sign of oestrus in mares (Arthur et al., 1996) nevertheless in the present study the said signs were not observed.
 

Table 1: Behavioural signs of oestrus in female Manipuri pony.


 
Occurrence of seasonality of oestrus
 
It was observed that the Manipuri pony mares showed the occurrence of oestrus were highest during spring (46.67%)  and summer (43.33%) seasons and the occurrence of oestrus was maximum during March to June (16.67%) and no oestrus was recorded from the month of October to January which was similar with the findings of Strickland, (1996) and Hughes et al., (1975) stated that in northern hemisphere the breeding season starts from April to September in addition Ginther (1992) accounted the official breeding season as from February to June. Contri et al., (2014) also found an increase oestrous length during spring and summer than autumn and winter seasons in female donkeys besides horses are seasonal breeders with extensive sexual activity in spring, summer and autumn (Talluri et al., 2016).There are reports on mares cycling throughout the year under certain conditions. During winter anoestrus the incidence of oestrus behaviour and ovulation is low (Ginther, 1974).
 
Parturition behaviour study
 
Pre-partum physical signs
 
Occurrences of pre-partum physical signs in female Manipuri pony are shown in (Table 2 and Fig 1). Pre-partum signs exhibited by all mares corroborates with the findings of Forsyth et al., (1975) and Blanchard et al., (2003).The behavioural signs of foaling observed in the present study during the different stages were similar with the findings of Blanchard et al., (2003), Morel (2003), Thangamani et al., (2018) and Purohit (2019).
 

Table 2: Occurrence of various pre-partum physical signs in female Manipuri pony.


 

Fig 1: Waxing of the teats.


 
Signs of parturition
 
Behavioural signs including restlessness, isolation from human and herd mates, frequent lying down and getting up, abdominal straining, tail raising and inappetance were shown during first stage of parturition (Table 3, 4 and Fig 2). Results showed that all the experimental mares show evidence of amniotic bag at the vulva, lateral recumbency with appearance of fetal legs and abdominal straining. The outcomes are similar with the report findings of Amann et al., (1989) and Morel (2003).
 

Table 3: Behavioural signs during first stage of parturition in female Manipuri pony.


 

Table 4: Behavioural signs during the onset of second stage of parturition in Manipuri pony.


 

Fig 2: Presence of water bag and escaping of allantoic fluids.


       
Behavioural signs during the end of the second stage of parturition in female Manipuri pony are depicted in the (Table 5 and Fig 3, 4). Behavioural signs during third stage of parturition in female Manipuri pony are depicted in the (Table 6). Results revealed that 90.00% had normal expulsion of placenta with an incidence of retention of placenta (10.00%). In the present study the behavioural signs of foaling observed were almost similar with the findings of Blanchard et al., (2003), Morel (2003), Meliani et al., (2013) and Purohit, (2019).
 

Table 5: Behavioural signs during the end of second stage of par turition in Manipuri pony.


 

Fig 3: One limb of foal precedes the other and muzzle of the foal was positioned at carpal level.


 

Fig 4: Head and limbs of the foal expelled out.


 

Table 6: Behavioural signs during third stage of parturition in Manipuri pony.


 
Placental characteristics
 
In the present study, the chorionic membrane of placenta was red colour and the amnion is almost translucent bluish colour and it was of micro cotyledonary diffuse placenta. Similar observations had been made by Morel (2003) and Thangamani et al., (2018) (Fig 5).
 

Fig 5: Outer view of the voided placenta.


 
Timing of foaling
 
Twenty percent of foaling occurred between 6 pm and 12 midnight and remaining occurred between 12 midnight and 6 am (Table 7). As per the published studies, the primary foaling time peak was around 2 am and the secondary smaller peak around 1 pm but generally most of the foaling happened at night hours. In the present study the time of foaling observed were almost similar with the findings of Meliani et al., (2013) which was influenced by environmental factors such as the month of birth, maternal mechanism factor of foaling and the sex of the foal Meliani et al., (2013).
 

Table 7: Occurrence of timing of birth.

Behavioural signs of oestrus were not prominent in Manipuri pony mares. The two clinically noticeable external signs exhibited by mares during oestrus consist of vulva swelling, vaginal mucous secretion where the most important sign being winking of clitoris was not observed. It was bring to a close that the behavioural signs of parturition in Manipuri pony mares were prominent however they typically gave birth during silent hours of night time and noticed that human interference results in disturbance particularly during the straining stage in first stage of parturition furthermore aggravating the condition leading to repulsion of placenta.

  1. Amann, S.F., Threlfall, W.R. and Kline, R.C. (1989). Equine temperature and progesterone fluctuations during estrus and near parturition. Theriogenology. 31: 1007-1019.

  2. Arthur, G.H., Noakes, D.E., Pearson, H. and Parkinson, T.J. (1996). Veterinary Reproduction and Obstetrics, 7th edn. Saunders publications, London, England, pp.10.

  3. Blanchard, T.L., Dickson, D.V., James, S., Charles, C.L., Steven, P.B. and Sherri, L.R. (2003). Manual of Equine Reproduction, 2nd edn. Science Direct, Elseivier, United States of America, pp.1-35.

  4. Contri, A., Robbe, D., Gloria, A., Amicis, I.D., Veronesi, M.L. and Carluccio, A. (2014). Effect of the Season on Some Aspects of the Oestrous Cycle in Martina Franca Donkey. Theriogenology. 1-5.

  5. Emady, M. (1999). A preliminary study on the estrous cycle of Caspian and Darashuri mares in Iran. Iran Agricultural Research. 18: 63-70.

  6. Forsyth, I.A., Rossdale, P.D. and Thomas, C.R. (1975). Studies on milk composition and lactogenic hormones in the mare. Journal of Reproduction and Fertility, Supplement. 23: 631-635.

  7. Ginther, D.J. (1974). Occurrence of anestrus, estrus, diestrus and ovulation over a 12 month period on mares. American. Journal of Veterinary Research. 35: 1173-1179.

  8. Ginther, O.J. (1992). Reproductive Biology of the Mare; Basic and Applied Aspects. Equiservices publications, Cross Plains, Wisconsis. 

  9. Hughes, J.P., Stabenfeldt, G.H. and Evans, J.W. (1975). The oestrous cycle of the mare. Journal of Reproduction and Fertility Supplement. 23: 161-166.

  10. Meliani, S., Benallou, B., Halbouche, M. and Haddouche, Z. (2013). Time of foaling in Arabian mares raised in Tiaret, Algeria. Asian Pacific Journal of Tropical Biomedicine. 3(7): 587-588.

  11. Morel. M.C.G.D. (2003). Equine Reproductive Physiology, Breeding and Stud Management. 2nd edition. CABI Publishing, United Kingdom. pp. 74-82.

  12. Panwar, B.S and Yadav, K.N. (2010). Equine Husbandry and Equestrian Sports, 1st edition. IBDC Publishers, Lucknow, India. pp. 50-77

  13. Purohit, G.N. (2019). Intra-partum conditions and their management in mare. Livestock Science. 2: 20-37.

  14. Strickland, C. (1996). Anatomy and Physiology of a Mare’s Reproductive System- The Horse, pp. 4-12. https://thehorse.com/14840/anatomy-and-physiology-of-a-mares-reproductive-system

  15. Taberner, E., Medrano, A., Pena, A., Rigau, T. and Miro, J. (2008). Oestrus cycle characteristics and prediction of ovulation in Catalonian jennies. Theriogenology. 70: 1489-1497.

  16. Talluri, T.R., Arangasamy, A., Singh, J., Ravi, S.K., Pal, Y., Legha, R.A., Raj, M.A., Chopra, A., Singh, R.K. and Tripathi, B.N. (2016). Factors affecting length of gestation in artificially inseminated Marwari mares in India. Asian Pacific Journal of Reproduction. 5(6): 481-489.

  17. Thangamani, A., Srinivas, M., Chandra, P.B. and Kumar, L.P. (2018). Periparturient event and dystocia in equine a review. International Journal of Environmental Science and Technology. 7(2): 648-658.

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