Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 55 issue 9 (september 2021) : 1072-1078

Molecular Detection and Risk Factor Analysis of Babesia gibsoni and Babesia vogeli in Naturally Infected Dogs in Andhra Pradesh, India

Jalajakshi Kopparthi, Sreedevi Chennuru, Chengalva Rayulu Vukka, Nalini Kumari Karumuri, Rani Prameela Devalam
1College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517 502, Andhra Pradesh, India. 
Cite article:- Kopparthi Jalajakshi, Chennuru Sreedevi, Vukka Rayulu Chengalva, Karumuri Kumari Nalini, Devalam Prameela Rani (2021). Molecular Detection and Risk Factor Analysis of Babesia gibsoni and Babesia vogeli in Naturally Infected Dogs in Andhra Pradesh, India. Indian Journal of Animal Research. 55(9): 1072-1078. doi: 10.18805/IJAR.B-4504.
Background: Babesiosis in dogs is endemic in tropical countries like India because of presence of tick vectors as well as reservoir stray dog population round the year. There is paucity of information regarding the prevalence and molecular confirmation of Babesia species in dogs in Andhra Pradesh. The present study was aimed to detect Babesia species in naturally infected dogs in Andhra Pradesh.
Methods: During the one-year study i.e. from June 2019 to July 2020, blood samples were collected from 442 tick infected dogs showing clinical signs suggestive of babesiosis to determine the prevalence of Babesia species by microscopy and molecular technique. Factors associated with prevalence of babesiosis were analyzed by logistic regression models.
Result: Microscopic examination of stained blood smears revealed 11.8% prevalence of Babesia spp. infection in dogs in the study area, while 28.3% of dogs were found to be positive for Babesia spp. in the first Babesia genus-specific PCR. Further analysis of positive samples with species-specific PCR, Babesia gibsoni and B. vogeli were detected 19.9% and 8.6% of dogs, respectively. Risk factor analysis by binary logistic regression model revealed that the living condition of the dogs, gender, age, medium and tick infestation as risk factors for B. gibsoni and B. vogeli infection.
  1. Abd Rani, P.A.M., Irwin, P.J., Coleman, G.T., Gatne, M. and Traub, R.J. (2011). A survey of canine tick- borne diseases in India. Parasites and Vectors. 4 (141): 1-7. 
  2. Acosta-Jamett, G., Cleaveland, S., Cunningham, A. and Bronsvoort, M., (2010). Demography of domestic dogs in rural and urban areas in coquimbo region of Chile and its implication for diseases transmission. Preventive Veterinary Medicine. 94: 272-281.
  3. Balachandran, C., Sridhar, R., Pazhanivel, N. and Anoopraj, R. (2010). Anote on the incidence of Babesia canis in a 10 day old pup on postmortem examination in Chennai, Tamil Nadu. Indian Journal Animal Research. 44(1): 73-75.
  4. Bashir, I.N., Chaudhry, Z.I., Ahmed, S. and Saeed, M.A. (2009). Epidemiological and vector identification studies on canine babesiosis. Pakistan Veterinary Journal. 29(2): 51-54.
  5. Betgiri, A.A., Jadhav, S.N., Pawde, M., Shukla, A., Mote, C., Pawar, P.D., Shanmugam, D. and Kundu, K. (2019). Mitochondrial cytochrome oxidase c subunit III (cox3) gene as a sensitive and specific target for molecular detection of Babesia gibsoni infection in dogs. Experimental Parasitology. 206: ISSN 0014-4894. 
  6. Duarte, S.C. Linhares, G.F.C. Romanowsky, T.N. Neto, O. and Borges, L.M.F. (2008). Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay. Veterinary Parasitology. 152(1-2): 16-20. 
  7. Fukumoto, S., Hiroshi, S., Igarashi, I. and Xuan X. (2005). Fatal experimental transplacental Babesia gibsoni infections in dogs. International Journal for Parasitology. 35(9): 1031-1035. 
  8. Gautam Patra, Ana, S., Ghosh S., Behara P., Borthakur, S.K., Biswas, P., Apurba. D. and Seikh, A. (2020). Prevalence of tick-borne pathogens in domestic dogs in North-Eastern region of India. Biological Rhythm Research. 51(2): 1-10.
  9. Happi, A.N., Toepp, A.J., Ugwu, C.A., Petersen, C.A. and Sykes, J.E. (2018). Detection and identification of blood-borne infections in dogs in Nigeria using light microscopy and the polymerase chain reaction. Veterinary Parasitology: Regional Studies and Reports. 11: 55-60. 
  10. Inokuma, H.Y., Yoshizaki, Y., Matsumoto, M., Okuda, T., Onishi, K., Nakagome, R., Kosugi. and Hirakrwa, M. (2004). Molecular survey of Babesia infection in dogs in Okinawa, Japan. Veterinary Parasitology. 121: 341-346. 
  11. Irwin, P.J. and Hutchinson, G.W. (1991). Clinical and pathological findings of Babesia infection in dogs. Australian Veterinary Journal. 68: 204-209. 
  12. Jain Jose, K., Lakshmanan, B., Hitaishi, V.,Nagaraj, Praveena. J.E., Syamala, K. and Aravindakshan, T. (2018). Detection of Babesia canis vogeli, Babesia gibsoni and Ehrlichia canis by multiplex PCR in naturally infected dogs in South India. Veterinarski Arhiv. 88(2): 215-224. 
  13. Jefferies, R., Ryan, U.M., Jardine, J., Broughton, D.K., Robertson, I.D. and Irwin, P.J. (2007). Blood, bull Terriers and Babesiosis: further evidence for direct transmission of Babesia gibsoni in dogs. Australian Veterinary Journal. 85(11): 459-463.
  14. Kalaivanan, M., Saravanan, S. and Ponnudurai, G. (2018). Identification of Babesia gibsoni in dogs from Namakkal region by polymerase chain reaction. Journal of Entomology and Zoology Studies. 6 (5):1146-1148.
  15. Kledmanee, K., Suwanpakdee, S., Krajangwong, S., Chatsiriwech, J., Suksai, P., Suwannachat, P., Sariya, L., Buddhirongawatr, R., Charoonrut, P and Chaichoun, K. (2009). Development of multiplex polymerase chain reaction for the detection of Ehrlichia canis, Babesia spp. and Hepatozoon canis in canine blood. South Asian Journal of Tropical Medicine and Public Health. 40(1): 35-39.
  16. Konto, M., Biu, A.A., Ahmed, M.I., Mbaya, A.W and Luka, J. (2014). Clinico-biochemical responses of dogs to experimental infection with Babesia canis. Veterinary World. 7(3): 113-    118.
  17. Kushwaha, N., Mondal, D., Singh, K.P. and Mahapatra, R.R. (2018). Comparative evaluation of different diagnostic tests for Babesia gibsoni in dogs. DOI:10.18805/ijar.3413.
  18. Laha, R., Bhattacharjee, K., Sarmah, P.C., Das, M., Goswami, A., Sarma, D. and Sen, A. (2014). Babesia infection in naturally exposed pet dogs from north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction. Journal of Parasitic Diseases. 38: 389-393. 
  19. Lavanya, K.V., Puttalakshmamma, G.C., Yogisharadhya, R., Mohan, H.V., Lakkundi, J.N., Manjunatha Reddy, G.B. (2019a). Development of cytochrome b based PCR and epidemiology of B. gibsoni in dogs. Journal of Experimental Biology and Agricultural Sciences. 7(4): 411-417. 
  20. Lavanya, K.V., Puttalakshmamma, G.C., Dhanalakshmi, H., Ananda, K.J., Mohan, H.V., Yathish, H.M., Manjunatha reddy, G. (2019b). Prevalence and molecular phylogenetic analysis of Babesia vogeli from dogs in Karnataka. Indian Journal of Veterinary Pathology. 43(2): 104.
  21. Maele, I.V.D., Bataille, I., Karine, K.S., Gielen, I., Daminet, S. (2008). An unusual form of canine babesiosis. The Canadian Veterinary Journal. 49:283-6.
  22. Mahalingaiah, M.K.C., Asoor, M., Thimmaiah, R.P., Narayanaswamy, H.D. and Mukartal, S.Y., Elattuvalappil, A.M., Chikkahonnaiah, N., Gupta, S., Singh, S. (2017). Prevalence of canine babesiosis in different breeds of dogs in and around Bangaluru. Advances in Animal and Veterinary Sciences. 5(3): 140-144. 
  23. Manoj, R.R.S, latta, R. Latrofa, M.S., Capozzi, L., Raman, M., Colella, V. and Otranto, D. (2020). Canine vector-borne pathogens from dogs and ticks from Tamil Nadu, India. Acta Tropica. 203: 105308. 
  24. Mittal, M., Kundu, K., Chakravarti, S., Mohapatra, J.K., Singh, V.K., Raja Kumar, B., Thakur, V., Churamani, C.P. and Kumar, A. (2019). Canine babesiosis among working dogs of organised kennels in India: A comprehensive haematological, biochemical, clinicopathological and molecular epidemiological multiregional study. Preventive Veterinary Medicine. 1; 169: 104696. doi: 10.1016/j.prevetmed.2019.104696.
  25. Muhammad, A.S., Raheela, A,.,Muhammad, L., Muhammad, I.R, Haroon, A., Wasim, S.,Fareeha, A., Saher, I., Shahid, H.F. and Uzma, F.D. (2018). First report on optimization of loop-mediated isothermal amplification (LAMP) for the diagnosis of Babesia felis. Indian Journal of Animal Research. 52: 401-404.
  26. Nimisha, M., Devassy, J.K. and Ravindran, R. (2019). Ticks and accompanying pathogens of domestic and wild animals of Kerala, South India. Experimental and Applied Acarology. 79: 137-155. 
  27. Senthil Kumar, K., Vairamuthu, S. and Kathiresan, D. (2009). Prevalence of haemoprotozoans in canines in Chenni city. Tamilnadu Journal of Veterinary and Animal Sciences. 5(3): 104-108. 
  28. Shaw, E.S., Michael, J.D., Birtles, R.J., Edward, B.B. (2001). Tick-borne diseases of dogs. Trends in Parasitology. 17(2): 74-80. 
  29. Singh, A., Singh, H., Singh, N.K., Singh, N.D. and Rath, S.S. (2014). Canine babesiosis in northwestern India: molecular detection and assessment of risk factors. Bio-Med Research International. 2014: 1-5.
  30. Solano-Gallego, L., Sainz, A., Roura, X., Estrada-Peria, A. and Miro, G. (2016). A review of canine babesiosis: The European perspective. Parasites and Vectors. 9: 336-354. 
  31. Soulsby, E.J.L. (1982). Helminths, Arthropods and Protozoa of Demesticated Animals. 7th ed. ELBS, Bailliers Tindall and Cassel, London. 
  32. Stegeman, J.R., Birkenheuer, A.J., Kruger, J.and Breitschwerdt, E. (2003). Transfusion-associated Babesia gibsoni infection in a dog. Journal of the American Veterinary Medical Association. 222(7): 959-963.
  33. Taneja, V. (2018). Sex hormones determine immune response. Frontiers in Immunology. 9: 1931. 
  34. Penzhorn, B.L. (2011). Why is southern african canine babesiosis so virulent? An evolutionary perspective. Parasites and Vectors. 4: 51. 
  35. Vairamuthu, S., Ranju, R.S., Latha, B.R., Dhivya, B. and Balachandran, C. (2014). A six year (2006-2011) retrospective study of haemoprotozoan parasites affecting dogs in Chennai, Tamil Nadu, India. Journal of Parasitic Diseases. 38(2): 193-195. 
  36. Zuk, M. and McKean, K.A. (1996). Sex differences in parasitic infections: patterns and processes. International Journal of Parasitology. 26: 1009-1024.

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