Loading...

Ameliorative, Antioxidant and Immunomodulatory Potential of Vitamin D on Aminoglycoside Induced Acute Kidney Injury in Wistar Rats

DOI: 10.18805/IJAR.B-4471    | Article Id: B-4471 | Page : 655-661
Citation :- Ameliorative, Antioxidant and Immunomodulatory Potential of Vitamin D on Aminoglycoside Induced Acute Kidney Injury in Wistar Rats.Indian Journal of Animal Research.2022.(56):655-661
Neeraj Thakur, S.K. Shukla, A.H. Ahmad, N.S. Jadon, J.L. Singh, G.E. Chethan niju.thakur92@gmail.com
Address : Department of Veterinary Clinical Complex, Faculty of Veterinary and Animal Sciences, Rajiv Gandhi South Campus-Banaras Hindu University, Barkachha, Mirzapur-231 001, Uttar Pradesh, India.
Submitted Date : 2-04-2021
Accepted Date : 15-06-2021

Abstract

Background: Acute kidney injury causes an abrupt decline in renal filtration and affects animals in a similar way to humans. Diagnosis can be made based on urinalysis, serum biochemistry and various biomarkers. The present study was conducted to evaluate the ameliorative, antioxidant and immunomodulatory potential of vitamin D in rats induced with acute kidney injury.
Methods: In the present study, group A rats were taken as healthy control, group B rats were given gentamicin @ 100 mg/kg BW intraperitoneally for 8 days and were considered as disease control and group C rats were treated with Vitamin D @ 0.4 µg/kg/day subcutaneously for 8 days along with intraperitoneal gentamicin injection. Reduced glutathione (GSH), lipid peroxide (LPO), catalase and superoxide dismutase (SOD) were estimated in erythrocytes on day 0, 4 and 8. Tumor necrosis factor alpha (TNF α) and interleukin 10 (IL 10) were also estimated along with urine and serum biochemistry on day 0, 4 and 8. Kidney tissue samples were collected on day 8 for histopathological examination.
Result: The mean values of GSH, catalase and SOD were significantly (P<0.05) higher whereas the mean value of LPO was significantly (P<0.05) lower in group C compared to group B on day 4 and 8. On day 4 and 8, the mean value of TNF α was significantly (P<0.05) lower, while the mean value of IL-10 was significantly (P<0.05) higher in rats treated with vitamin D as compared to disease control. Histopathological examination along with urine and serum biochemistry revealed protective efficacy of vitamin D in acute kidney injury. Based on the findings of the present study, it is concluded that vitamin D is having ameliorative efficacy along with antioxidant and immunomodulatory potential in case of gentamicin induced acute kidney injury in Wistar rats. However, detailed studies are required to explore the therapeutic potential of vitamin D in clinical cases of kidney diseases.

Keywords

​Acute kidney injury Gentamicin IL-10 TNF-á Vitamin D


References

  1. Andrew, B. Braun, Christopher, K.K. (2013). Vitamin D in acute kidney injury. Inflammation and Allergy-Drug Targets (Formerly Current Drug Targets-Inflammation and Allergy) (Discontinued). 12(4): 262-272.
  2. Bae, E.H., Kim, I.J., Joo, S.Y., Kim, E.Y., Choi, J.S., Kim, C.S., Ma, S.K., Lee, J., Kim, S.W. (2014). Renoprotective effects of the direct renin inhibitor aliskiren on gentamicin- induced nephrotoxicity in rats. Journal of the Renin- Angiotensin-Aldosterone System. 15(4): 348-361. 
  3. Bao, Y.W., Yuan, Y., Chen, J.H., Lin, W.Q. (2018). Kidney disease models: tools to identify mechanisms and potential therapeutic targets. Zoological Research. 39(2): 72. 
  4. Bergmayer, H.U. (1983). UV method of catalase assay. Methods of enzymatic analysis. 3: 273.
  5. Culling, C.F.A. (1974). Handbook of Histopathological and Histochemical Techniques, 3rd ed. Butter Worth, Co. Ltd., Great Britain.
  6. Eirin, A., Lerman, A., Lerman, L.O. (2016). The emerging role of mitochondrial targeting in kidney disease. Pharmacology of Mitochondria. 229-250.
  7. Gyurászová, M., Kovalèíková, A.G., Renczés, E., Kmeová, K., Celec, P., Bábíèková, J., Tóthová, L'. (2019). Oxidative stress in animal models of acute and chronic renal failure. Disease markers. 
  8. Hamzawy, M., Gouda, S.A.A., Rashed, L., Morcos, M.A., Shoukry, H., Sharawy, N. (2019). 22-oxacalcitriol prevents acute kidney injury via inhibition of apoptosis and enhancement of autophagy. Clinical and Experimental Nephrology. 23(1): 43-55.
  9. Heine, G., Niesner, U., Chang, H.D., Steinmeyer, A., Zügel, U., Zuberbier, T., Radbruch, A., Worm, M. (2008). 1, 25 dihydroxyvitamin D3 promotes IL 10 production in human B cells. European Journal of Immunology. 38(8): 2210- 2218.
  10. Hur, E., Garip, A., Camyar, A., Ilgun, S., Ozisik, M., Tuna, S., Olukman, M., NarliOzdemir, Z., Yildirim Sozmen, E., Sen, S., Akcicek, F. (2013). The effects of vitamin D on gentamicin -induced acute kidney injury in experimental rat model.  International Journal of Endocrinology.
  11. Korf, H., Wenes, M., Stijlemans, B., Takiishi, T., Robert, S., Miani, M., Eizirik, D.L., Gysemans, C., Mathieu, C. (2012). 1, 25-Dihydroxyvitamin D3 curtails the inflammatory and T cell stimulatory capacity of macrophages through an IL- 10-dependent mechanism. Immunobiology. 217(12): 1292-1300.
  12. Kushwaha, V., Sharma, M., Vishwakarma, P., Saini, M., Saxena, K. (2016). Biochemical assessment of nephroprotective and nephrocurative activity of Withania somnifera on gentamicin-induced nephrotoxicity in experimental rats. International Journal of Research in Medical Sciences. 4: 298-302.
  13. Lewington, A.J., Cerdá, J., Mehta, R.L. (2013). Raising awareness of acute kidney injury: a global perspective of a silent killer. Kidney International. 84(3): 457-467.
  14. Li, Y.C., Kong, J., Wei, M., Chen, Z.F., Liu, S.Q., Cao, L.P. (2002). 1, 25-Dihydroxyvitamin D3 is a negative endocrine regulator of the renin-angiotensin system. The Journal of clinical Investigation. 110(2): 229-238.
  15. Lopez-Novoa, J.M., Quiros, Y., Vicente, L., Morales, A.I., Lopez- Hernandez, F.J. (2011). New insights into the mechanism of aminoglycoside nephrotoxicity: an integrative point of view. Kidney International. 79(1): 33-45.
  16. Madesh, M., Balasubramanian, K.A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Biophysics.  35(3): 184-188.
  17. Maldonado, P.D., Barrera, D., Rivero, I., Mata, R., Medina-Campos, O.N., Hernández-Pando, R., Pedraza-Chaverrí, J. (2003). Antioxidant S-allylcysteine prevents gentamicin-induced oxidative stress and renal damage. Free Radical Biology and Medicine. 35(3): 317-324.
  18. Nandi, A., Yan, L.J., Jana, C.K., Das, N. (2019). Role of catalase in oxidative stress-and age-associated degenerative diseases. Oxidative Medicine and Cellular Longevity. 1-19.
  19. Overbergh, L., Decallonne, B., Valckx, D., Verstuyf, A., Depovere, J., Laureys, J., Rutgeerts, O., Saint Arnaud, R., Bouillon, R., Mathieu, C. (2000). Identification and immune regulation of 25 hydroxyvitamin D 1 á hydroxylase in murine macrophages. Clinical and Experimental Immunology. 120(1): 139-146.
  20. Parlakpinar, H., Tasdemir, S., Polat, A., Bay-Karabulut, A., Vardi, N., Ucar, M., Acet, A. (2005). Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats. Toxicology. 207(2): 169-177.
  21. Pedraza-Chaverrýì, J., Maldonado, P.D., Medina-Campos, O.N., Olivares-Corichi, I.M., de los Ángeles Granados-Silvestre, M., Hernández-Pando, R., Ibarra-Rubio, M.E. (2000). Garlic ameliorates gentamicin nephrotoxicity: relation to antioxidant enzymes. Free Radical Biology and Medicine. 29(7): 602-611.
  22. Placer, Z.A., Cushman, L.L., Johnson, B.C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical biochemistry. 16(2): 359-364.
  23. Prins, H.K., Loos, J.A. (1969). Glutathione. In Biochemical methods in red cell genetics. Academic Press New York. Pp. 115- 137.
  24. Rahman, M., Shad, F., Smith, M.C. (2012). Acute kidney injury: a guide to diagnosis and management. American Family Physician. 86(7): 631-639.
  25. Richterich, R. (1969). Clinical chemistry: Theory and Practice Karger, Basel (Switzerland) Academic Press, NY and London. Pp. 336-337.
  26. Safa, J., Argani, H., Bastani, B., Nezami, N., Rahimi, A.B., Ghorbani, H.A., Kalagheichi, H., Amirfirouzi, A., Mesgari, M., Solaeymanirad, J. (2010). Protective effect of grape seed extract on gentamicin-induced acute kidney injury. Iranian Journal of Kidney Diseases. 4(4): 285-291.
  27. Silverblatt, F.J., Kuehn, C. (1979). Autoradiography of gentamicin uptake by the rat proximal tubule cell. Kidney International. 15(4): 335-345.
  28. Udupa, V., Prakash, V. (2019). Gentamicin induced acute renal damage and its evaluation using urinary biomarkers in rats. Toxicology reports. 6: 91-99.
  29. Wiseman, H. (1993). Vitamin D is a membrane antioxidant Ability to inhibit iron dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS letters. 326(1-3): 285-288.
  30. Zhang, L., Wang, F., Wang, L., Wang, W., Liu, B., Liu, J., Chen, M., He, Q., Liao, Y., Yu, X., Chen, N. (2012). Prevalence of chronic kidney disease in China: a cross-sectional survey. The Lancet. 379(9818): 815-822.

Global Footprints