Loading...

Biochemical Profile, Micro-mineral Status and Metallothionein Expression in Abattoir Buffaloes Environmentally Exposed to Heavy Metals

DOI: 10.18805/IJAR.B-4464    | Article Id: B-4464 | Page : 416-423
Citation :- Biochemical Profile, Micro-mineral Status and Metallothionein Expression in Abattoir Buffaloes Environmentally Exposed to Heavy Metals.Indian Journal of Animal Research.2022.(56):416-423
Himalaya Bhardwaj, Chanchal Singh, Shashi Nayyar himalaya530@gmail.com
Address : Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences, Ludhiana-141 004, Punjab, India.
Submitted Date : 26-03-2021
Accepted Date : 14-04-2021

Abstract

Background: The present study was planned to assess the biochemical and micro-minerals profile in blood and other tissues of buffaloes environmentally exposed to heavy metals. 
Methods: Tissues (liver and kidney) and blood samples (n=50) were collected from local abattoir. Based on the level of heavy metals, animals were classified as exposed and control was found to have heavy metals in normal range. Blood and tissue sample from both groups were analyzed for micro-minerals, antioxidant status, metabolic profile and expression of metallothionein-2 (MT-2).
Result: Exposed group was found to have significantly (p<0.05) higher level of arsenic and chromium as compared to the control group. Level of Copper (Cu) and zinc (Zn) were observed to be significantly (p<0.05) higher in exposed animals as compared to control but their concentrations were below the permissible limit in both the groups. Cobalt (Co) and iron (Fe) level were normal in all tissues but Fe level was lower than permissible limit in blood. Malonldialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) was found to be significantly increased (p<0.05) in tissues and blood of exposed group. The exposed buffaloes were found to have significantly (p<0.05) increased glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphatase (ALP), urea and creatinine level as compared to control group. Fold change expression of metallothionein (MT-2), had maximum in liver, followed by kidney and blood as compared to control group. The study concluded that heavy metals exposure and low concentration of micro-minerals in buffaloes could result in oxidative damage and alterations in the expression of metallothionein.

Keywords

Buffalo Cu Co Fe LDH Metallothionein

References

  1. Ahmed, W., Hanafi, E., Abd, R.A., Sabra, A.H. (2009). Effect of copper deficiency on ovarian activity in egyptian buffalo-cows. World Journal of Zoology. 4(1): 01-08.
  2. ANZFA (Australia New Zealand Food Authority). Wellington NZ 6036. (2001). Retrieved from URL: http://www.Anzfa.Gov.all.
  3. Bhardwaj, H., Singh, C., Nayyar, S. (2021). Assessment of Adverse Effects of Lead, Nickel and Cadmium on Biochemical Parameters, Antioxidants Status and Metallothionein Expression in Buffaloes Slaughtered at Local Abattoir. Indian Journal of Animal Research. 10.18805/IJAR.B-4242.
  4. Dash, S., Nayyar, S., Jindal, R., Mukhopadhaya, C.S. (2016). Plasma enzyme activities in buffaloes (Bubalus bubalis) naturally exposed to arsenic contamination. Indian Veterinary Journal. 93: 42.
  5. Dash, S., Nayyar, S., Sodhi, S., Jindal, R. (2019). Terminalia arjuna modulates oxidative stress in buffaloes exposed to lead contaminated water and feed. Indian Veterinary Journal. 96(05): 17-20. 
  6. Demirezen, O., Uruc, K. (2006). Comparative study of trace elements in certain fish, meat and meat products. Food Chemistry. 32: 215-22.
  7. Dhaliwal, R.S., Sushma, C. (2016). Effect of Heavy Metals on Oxidative Stress Parameters of Cattle Inhabiting Buddha Nallah Area of Ludhiana District in Punjab. Journal of Veterinary Science and Technology. 7: 352. 
  8. Doyle, J.J., Spaulding, J.E. (1978). Toxic and essential trace elements in meat-a review. Journal of Animal Science. 47: 398-419. 
  9. EI-Salam, N.M., Ahmad, S., Basir, A., Rais, A.K., Bibi, A., Ullah, R., Shad, A.A., Muhammad, Z., Hussain, I. (2013). Distribution of heavy metals in the liver, kidney, heart, pancreas and meat of cow, buffalo, goat, sheep and chicken from Kohat market Pakistan. Life Sciences Journal. 10(7): 937-40.
  10. El-Nekeety, A.A., El-Kady, A.A., Soliman, M.S., Hassan, N.S., Abdei-    Wahhab, M.A. (2009). Protective effect of Aqualegia vulgaris (L.) against lead acetate-induced oxidative stress in rats. Food and Chemical Toxicology. 47: 2209-15.
  11. FAO. (2019). Livestock Production Indices. Food and Agriculture Organization of the United Nations. (http://www.fao.org/)
  12. Gurer, H., Ercal, N. (2000). Can antioxidants be beneficial in the treatment of lead poisoning? Free Radical Biology and Medicine. 29: 927-45.
  13. Hundal, H.S., Kumar, R., Singh, D., Manchanda, J.S. (2006). Available nutrient and heavy metal status of soils of Punjab, North West India. Journal of Indian Society of Soil Science. 54: 50-56.
  14. Javed, M., Ahmad, M.I., Usmani, N., Ahmad, M. (2017). Multiple biomarker responses (serum biochemistry, oxidative stress, genotoxicity and histopathology) in Channapunctatus exposed to heavy metal loaded waste water. Science Reports. 7: 1-8.
  15. Kagi, J.H.R. (1991). Overview of metallothionein. Methods Enzymology. 205: 613.
  16. Kaltreider, R.C., Davis, A.M., Lriviere, J.P. and Hamilton, J.W. (2001). Arsenic alters the function of the glucocorticoid receptor as a transcription factor. Environmental Health Perspectives. 109: 245-51.
  17. Kaneko, J.J., Harvey, J.W. and Bruss, M.L. (1997). Clinical Biochemistry of Domestic Animals. 6th Edn. Elsevier Inc., Oxford, United Kingdom.
  18. Kumar, M. and Padhy, P.K. (2013). Oxidative stress and heavy metals: an appraisal with reference to environmental biology. International Research Journal of Biological Sciences. 2(10): 91-101.
  19. Li, Y., McCrory, F.D., Powell, J., Saam, H., Jackson-Smith, D. (2005). A survey of selected heavy metal concentrations in wisconsin dairy feeds. Journal of Dairy Science. 88: 2911-22.
  20. Liu, J., Cheng, M.L., Yang, Q., Shan, K.R., Shen, J., Zhou, Y., Waalkes, M.P. (2007). Blood metallothionein transcript as a biomarker for metal sensitivity: low blood metallothionein transcripts in arsenicosis patients from Guizhou, China. Environmental Health Perspectives. 115(7): 1101-1106. 
  21. Ludmila, D. (1976). Chemical analysis by atomic spectroscopy. Varian Techtron Pvt. Ltd., Melbourne, Australia.
  22. Margoshes, M., Vallee, B.L. (1957). A cadmium protein from equine kidney cortex. Journal of the American Chemical Society. 79: 4813-4814.
  23. Masters, B.A., Quaife, C.J., Erickson, J.C., Kelly, E.J., Froelick, G.J., Zambrowicz, B.P., Brinster, R.L., Palmiter, R.D. (1994). Metallthionein-III is expressed in neurons that sequester zinc in synaptic vesicles. Journal of Neuroscience. 14(10): 5844-57. 
  24. McDowell, L.R. (2012). Vitamin B12. In Vitamins in Animal and Human Nutrition; Iowa State University Press: Ames, IA, USA, 523-563.
  25. Mohajeri, G., Norouzian, M.A., Mohseni, M., Afzalzadeh, A. (2014). Changes in blood metals, hematology and hepatic enzyme activities in lactating cows reared in the vicinity of a lead-zinc smelter. Bulletin of Environmental Contamination and Toxicology. 92: 693.
  26. Nygard, A., Jørgensen, C.B., Cirera, S. (2007). Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR. BMC Molecular Biology. 8: 67 https://doi.org/10.1186/1471-2199-8-67.
  27. Panigrahi, A., Dasmahapatra, A., Medda, A.K. (2016). Responsiveness of total plasma protein after administration of some toxic heavy metals in an Indian teleost (Clariasbatrachus L.). American Journal of Bio Science. 4(5): 64-73.
  28. Petra, S., Vlasta, S., Leos, P., Lubica, M., Rudolf D. (2011). Effect of inorganic and organic zinc supplementation on coccidial infections in goat kids. Acta Veterinaria Brno. 80: 131-37.
  29. Puls, R. (1994). Mineral Levels in Animal Health. Diagnostic Data, 2nd edn. Sherpa International, Claerbrook, BC, Canada.
  30. Rana, T., Bera, A.K., Das, S., Bhattacharya, D., Bandopadhyay, D.P., Das, S.K. (2010). Effect of chronic intake of arsenic-contaminated water on blood oxidative stress indices in cattle in an arsenic-affected zone. Ecotoxicology and Environmental Safety. 73:327.
  31. Roy, D., Das, T.K., Vaswani, S. (2013). Arsenic: it’s extent of pollution and toxicosis: an animal perspective. Veterinary World. 6(1): 53-58.
  32. Ruttkay-Nedecky, B., Nejdl, L., Gumulec, J., Zitka, O., Masarik, M., Eckschlager, T., Stiborova, M., Adam, V., Kizek, R. (2013). The role of metallothionein in oxidative stress. International Journal of Molecular Science. 14: 6044.
  33. Shaw, C.F., Savas, M.M., Petering, D.H. (1991). Ligand substitution and sulfhydryl reactivity of metallothionein. Methods Enzymology. 205: 401.
  34. Sinha, M., Manna, P., Sil, P.C. (2008). Protective effect of arjunolic acid against arsenic-induced oxidative stress in mouse brain. Journal of Biochemical and Molecular Toxicology. 22(1): 15.
  35. Skalicka, M., Korenekova, B., Nad, P. (2005). Copper in livestock from polluted area. Bulletin of Environmental Contamination and Toxicology. 74: 740-44
  36. Somasundaram, J., Krishnasamy, R., Savithri, P. (2005). Biotransfer of heavy metals in Jersey cows. Indian Journal of Animal Sciences. 75:1257.
  37. SPSS Inc. Released (2007). SPSS for Windows, Version 16.0. Chicago, SPSS Inc.
  38. Šveikauskaitė, I., Šulinskienė, J., Sadauskiene, I., Ivanov, L. (2014). The effects of lead and nickel ions on total proteins and meta-llothioneins synthesis in mice liver. Biologija. 60: 2861.
  39. Winski, S.L., Carter, D.E. (1998). Arsenate toxicity in human erythrocytes: characterization of morphologic changes and determination of the mechanism of damage. Journal of Toxicology and Environmental Health-A. 53(5): 345-55.
  40. Yamanaka, K., Hasegawa, A., Sawamura, R., Okada, S. (1991). Cellular response to oxidative damage in lung induced by the administration of dimethylarsinic acid, a major metabolite of inorganic arsenics in mice. Toxicology and Applied Pharmacology. 108(2): 205-13.
  41. Yeotikar, P.V., Nayyar, S., Singh, C., Mukhopadhaya, C.S., Kakkar, S.S., Jindal, R. (2018). Levels of Heavy Metals in Drinking Water, Blood and Milk of Buffaloes during Summer and Winter Seasons in Ludhiana, Punjab (India). International Journal Pure and Applied Bioscience. 6(2):1265-1274. doi:http://dx.doi.org/10.18782/2320-7051.6416.

Global Footprints