Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 56 issue 8 (august 2022) : 917-920

PCR-RFLP Analysis of Single Nucleotide Polymorphism (SNP) C-2402T at the Promoter Region of Prolactin Gene and its Association with Positively Correlated Production Traits in White Leghorn Chicken

Azhaguraja Manoharan, S. Sankaralingam, P. Anitha, Binoj Chacko, T.V. Aravindakshan
1ICAR- Indian Veterinary Research Institute, Izzatnagar, Bareilly-243 122, Uttar Pradesh, India.
Cite article:- Manoharan Azhaguraja, Sankaralingam S., Anitha P., Chacko Binoj, Aravindakshan T.V. (2022). PCR-RFLP Analysis of Single Nucleotide Polymorphism (SNP) C-2402T at the Promoter Region of Prolactin Gene and its Association with Positively Correlated Production Traits in White Leghorn Chicken. Indian Journal of Animal Research. 56(8): 917-920. doi: 10.18805/IJAR.B-4391.
Background: The avian prolactin gene is highly conserved, located on chromosome number 2 and most sequence polymorphisms occurs in the 5’ flanking region, 3’ flanking region, and the coding region of signal peptide. The present study was aimed at the identification of SNP C-2402T of prolactin gene and its association with production traits in White Leghorn chicken.
Methods: A total of 200 birds of White Leghorn were selected from All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy. Genomic DNA was isolated from venous blood. Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis was done to identify the SNP C-2402T of prolactin gene.
Result: All the birds were observed with the same genotype CC and the frequency of the C allele was one.

  1. Bagheri Sarvestani, A.S., Niazi, A., Zamiri, M.J. and Dadpasand Taromsari, M. (2013). Polymorphisms of prolactin gene in a native chicken population and its association with egg production. Iranian Journal of Veterinary Research. 14: 113-119.

  2. Basic Animal Husbandry Statistics (2019). Government of India. Ministry of Fisheries, Animal Husbandry and Dairying. Department of Animal Husbandry and Dairying.

  3. Chau, T.V. (2016). Single nucleotide polymorphisms in candidate genes associated with egg production traits in native Noi chicken of Vietnam. International Journal of Plant, Animal and Environmental Sciences. 6: 162-169.

  4. Cui, J.X., Du, H.L., Liang, Y., Deng, X. M., Li, N. and Zhang, X.Q. (2006). Association of polymorphisms in the promoter region of chicken prolactin with egg production. Poultry Science. 85: 26-31.

  5. Fulton, J.E. (2012). Genomic selection for poultry breeding. Animal Frontiers. 2: 30-36.

  6. Government of India. (2020). Annual Report 2019-20. Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture.

  7. Jiang, R.S., Xu, G.Y., Zhang, X.Q. and Yang, N. (2005). Association of polymorphisms for prolactin and prolactin receptor genes with broody traits in chickens. Poultry Science. 84: 839-845.

  8. Kulibaba, R.A. (2015). Polymorphism of growth hormone, growth hormone receptor, prolactin and prolactin receptor genes in connection with egg production in Poltava clay chicken. Agricultural Biology. 50: 198-207.

  9. Kulibaba, R.A. and Podstreshnyi, A.P. (2012). Prolactin and growth hormone gene polymorphisms in chicken lines of Ukrainian selection. Cytology and Genetics. 46: 390-395.

  10. Kulibaba, R.O., Sakhatskyi, M.I., Liashenko, Y.V., Yurko, P.S. and Osadcha, Y.V. (2020). Functional genes polymorphism associations with egg quality traits in the populations of dual-purpose. Agricultural Science and Practice. 7: 14-23.

  11. Romanov, M.N. (2001). Genetics of broodiness in poultry-a review. Asian-Australasian Journal of Animal Sciences. 14: 1647-1654.

  12. Salisu, I.B., Olawale, A.S., Jabbar, B., Koloko, B.L., Abdurrahaman, S.L., Amin, A.B. and Ali, Q. (2018). Molecular markers and their potentials in animal breeding and genetics. Nigerian Journal of Animal Science. 20: 29-48.

  13. Tomar, A., Poonia, J., Chaudhari, M. and Kumar, P. (2015). Evaluation of production Performance of some economic traits in White Leghorn birds. Haryana Veterinarian. 54: 19-21.

  14. Wang C., Liang Z., Yu W., Feng Y., Peng X. and Gong Y. (2011). Polymorphism of the prolactin gene and its association with egg production traits in native Chinese ducks. South African Journal of Animal Science. 41: 64-69.

  15. Wilkanowska, A., Mazurowski, A., Mroczkowski, S. and Kokoszyñski, D. (2014). Prolactin (PRL) and prolactin receptor (PRLR) genes and their role in poultry production traits. Folia Biologica (Kraków). 62: 1-8.

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