Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 5 (october 2017) : 841-846

Differential gene expressions of NLRC5 signaling pathway in chicken macrophages cells response to Salmonella Enteritidis derived lipopplysaccharides stimulation

Lingling Qiu, Guobin Chang, Xiangping Liu, Zhiteng Li, Wei Lu, Lichen Ren, Lu Xu, Fang Wan, Xiaomin Guo, Guohong Chen
1<p>Poultry Institute, Chinese Academy of Agricultural Sciences,&nbsp;Yangzhou, Jiangsu 225003, P. R. China.</p>
Cite article:- Qiu Lingling, Chang Guobin, Liu Xiangping, Li Zhiteng, Lu Wei, Ren Lichen, Xu Lu, Wan Fang, Guo Xiaomin, Chen Guohong (2016). Differential gene expressions of NLRC5 signaling pathway in chicken macrophages cells response to Salmonella Enteritidisderived lipopplysaccharides stimulation . Indian Journal of Animal Research. 51(5): 841-846. doi: 10.18805/ijar.11462.

ABSTRACT

As we all known, NLRC5 recognizes intracellular pathogen-associated molecular patterns (PAMPs) and provokes innate immune system. Its role in innate immune response, NF-kB activation and MHC-I expression remains controversial. In the present study, it was detected that differential gene expressions in NLRC5 signaling pathway at 2, 4, 6 and 8 hours after exposure to LPS using qRT-PCR technology, then analyzed its roles in host defense. The results showed that, comparing to control groups, the expression of NLRC5, MHC-I and IL-18 in LPS-treated groups were significantly up-regulated at 2 hours post stimulation (hps), TLR4 and NF-kB showed conspicuously up-regulated at 4 hps, while STAT1 was significantly down-regulated at 8 hps. Collectively, LPS did evoke inflammatory responses and NLRC5 may negatively regulate NF-kB and critically regulate MHC-I to control intracellular PAMPs in chicken macrophage cell line but the specific role of NLRC5 in host defense relates to cell types and species tested.

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