Indian Journal Of Animal Research
Publish
your articles with us

Quick Facts



Payment Options

payment portals

Click here to pay directly

Expression analysis of IL-15 as a candidate gene for placental efficiency in goats

Cao-de Jiang, Xue-qiang Peng, Lu-fang Su, Yong-ju Zhao, Guang-xin E and Yong-fu Huang

Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing 400715, China.

jcdpjx@swu.edu.cn

Page Range:
192-197
Article ID:
B-438
Online Published:
8-05-2017
Abstract

In present study, functional relevance of IL-15 with goat placenta was determined by quantitative real-time PCR (SYBR Green chemistry) and western blot. The 486 bp full-length coding sequence of the gene, encoding 162 amino acids, was cloned. Expression of IL-15 was significantly higher in placenta, especially in cotyledon, than that in other neonatal tissues (P<0.05). In both Dazu Black and Inner Mongolia Cashmere breeds, IL-15 mRNA in cotyledon was up-regulated at term compare to that at day 30 of gestation (P<0.05), and was significantly different between both breeds (P<0.05). Additionally, IL-15 transcripts had a close relationship with that of vascular endothelial growth factor and a positive correlation with placental efficiency (R2=0.922, P=0.04), but it was negatively correlated with cotyledon density (R2=0.952, P=0.024). The results indicate that IL-15 is conserved in goats, and has important role in placenta, thereby can be a candidate gene for placental efficiency in goats.

Keywords
Expression analysis, Goat, IL-15, Placenta, RT-PCR.
References
  1. Agarwal, R., Loganath, A., Roy, A.C., Wong, Y.C. and Ng, S.C. (2001). Expression profiles of interleukin-15 in early and late gestational human placenta and in pre-eclamptic placenta. Mol. Hum. Reprod. 7:97-101.
  2. Borowicz, P.P., Arnold, D.R., Johnson, M.L., Grazul-Bilska, A.T., Redmer, D.A. and Reynolds, L.P. (2007). Placental growth throughout the last two thirds of pregnancy in sheep: vascular development and angiogenic factor expression. Biol. Reprod. 76:259-267.
  3. Chennazhi, K.P. and Nayak, N.R. (2009). Regulation of angiogenesis in the primate endometrium: vascular endothelial growth factor. Semin. Reprod. Med. 27:80-89. 
  4. Freking, B.A., Leymaster, K.A.,Vallet, J.L. and Christenson, R.K. (2007). Number of fetuses and conceptus growth throughout gestation in lines of pigs selected for ovulation rate or uterine capacity. J. Anim. Sci. 85:2093-2103.
  5. Giron-Michel, J., Azzi, S., Ferrini, S., Chouaib, S., Camussi, G., Eid, P. and Azzarone, B. (2013). Interleukin-15 is a major regulator of the cell-microenvironment interactions in human renal homeostasis. Cytokine Growth Factor Rev. 24:13-22.
  6. Jiang C., Shi P., Li, S., Dong, R., Tian, J., Wei, J. and Luo, S. (2010). Gene expression profiling of skeletal muscle of nursing piglets. Int. J. Biol. Sci. 6:627-638.
  7. Luo, N., Guan, D., Li, H., Wang, Y. and Zhao, Y. (2015). Placental cotyledon traits and structure and their relationship with reproductive performance in Dazu black goat (Capra Hircus). Chin. J. Vet. Sci. 35:1339-1344.
  8. Lv, S.J., Su, L., Li, H., Han., R.L., Sun, G.R. and Kang, X.T. (2012). Polymorphisms of the interleukin-15 gene and their associations with fatness and muscle fiber traits in chickens. J. Appl. Genet. 53:443-448.
  9. Mesa, H., Safranski, T.J., Fischer, K.A., Cammack, K.M. and Lamberson, W.R. (2005). Selection for placental efficiency in swine: genetic parameters and trends. J. Anim. Sci. 83:983-991.
  10. Ocak, S., Emsen, E., Köycegiz, F., Kutluca, M. and Onder, H. (2009). Comparison of placental traits and their relation to litter size and parity weight in sheep. J. Anim. Sci. 87:3196-3201.
  11. Quinn, L.B.S. and Anderson, B.G. (2011). Interleukin-15, IL-15 receptor-alpha, and obesity: concordance of laboratory animal and human genetic studies. J. Obes. 2011:456347.
  12. Sammin, D., Markey, B., Bassett, H. and Buxton, D. (2009). The ovine placenta and placentitis—A review. Veterinary Microbiology 135:90-97.
  13. Schmittgen, T.D. and Livak, K.J. (2008). Analyzing real-time PCR data by the comparative C(T) method. Nat. Protocols 3:1101-1108.
  14. Wang, L.J., Sun, X.W., Guo, F.Y., Zhao, Y.J., Zhang, Z.H., Zhao, Z.Q. (2016). Transcriptome analysis of the uniparous and multiparous goats ovaries. Reprod. Domest. Anim. Reprod. Dom. Anim. 51:1-9.
  15. Wang, Q.H., Chen, J.Y., Guo K.N., Zheng X.B., Wu Q., Wei H. and Liu Y. (2015). A correlation study of boar taint-related genes, boar taint substances and sex hormones in Bama miniature pigs at different ages. Indian J. Anim. Res. 49:745-751.
  16. Xiong, C., Hixson, P.M., Mendoza, L.H. and Smith, C.W. (2005). Cloning and expression of rabbit interleukin-15. Vet. Immunol. Immunopathol. 107:131-141.
  17. Zourbas, S., Dubanchet, S., Martal, J. and Chaouat, G. (2015). Localization of pro-inflammatory (IL-12, IL-15) and anti-    inflammatory (IL-11, IL-13) cytokines at the foetomaternal interface during murine pregnancy. Genome Biol. 16:112. 
Global footprints


© 2015 ARCC JOURNALS. All Rights Reserved. Powered By ARCC JOURNALS