Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Indian Journal of Animal Research, volume 52 issue 2 (february 2018) : 192-197

Expression analysis of IL-15 as a candidate gene for placental efficiency in goats

Cao-de Jiang, Xue-qiang Peng, Lu-fang Su, Yong-ju Zhao, Guang-xin E, Yong-fu Huang
1<p style="text-align: justify;">Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization,&nbsp;College of Animal Science and Technology, Southwest University, Chongqing 400715, China.</p>
Cite article:- Jiang Cao-de, Peng Xue-qiang, Su Lu-fang, Zhao Yong-ju, E Guang-xin, Huang Yong-fu (2017). Expression analysis of IL-15 as a candidate gene for placental efficiency in goats . Indian Journal of Animal Research. 52(2): 192-197. doi: 10.18805/ijar.v0iOF.7829.

In present study, functional relevance of IL-15 with goat placenta was determined by quantitative real-time PCR (SYBR Green chemistry) and western blot. The 486 bp full-length coding sequence of the gene, encoding 162 amino acids, was cloned. Expression of IL-15 was significantly higher in placenta, especially in cotyledon, than that in other neonatal tissues (P<0.05). In both Dazu Black and Inner Mongolia Cashmere breeds, IL-15 mRNA in cotyledon was up-regulated at term compare to that at day 30 of gestation (P<0.05), and was significantly different between both breeds (P<0.05). Additionally, IL-15 transcripts had a close relationship with that of vascular endothelial growth factor and a positive correlation with placental efficiency (R2=0.922, P=0.04), but it was negatively correlated with cotyledon density (R2=0.952, P=0.024). The results indicate that IL-15 is conserved in goats, and has important role in placenta, thereby can be a candidate gene for placental efficiency in goats.

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