Doppler Ultrasonographic Assessment of Maternal and Foetal Blood Flow in Canine Pregnancy and Its Application in the Critical Management of Gestation#

DOI: 10.18805/IJAR.B-4286    | Article Id: B-4286 | Page : 811-821
Citation :- Doppler Ultrasonographic Assessment of Maternal and Foetal Blood Flow in Canine Pregnancy and Its Application in the Critical Management of Gestation#.Indian Journal of Animal Research.2022.(56):811-821
Shibu Simon, A. Ramanathan, K.N. Aravinda Ghosh shibu@kvasu.ac.in
Address : Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy-680 651, Kerala, India. 
Submitted Date : 24-08-2020
Accepted Date : 10-12-2020


Background: Doppler ultrasonographic assessment of maternal and foetal blood flow/perfusion has become an indispensable diagnostic tool in veterinary obstetrics. Periodic Doppler examination of critical canine pregnancies to interpret vascular information such as blood velocity, flow direction/pattern and vascular pathologies would ensure safety of dam and survival chances of conceptuses.
Methods: The present study included twenty apparently healthy bitches of different breeds presented at University Veterinary Hospital, Thrissur, Kerala for the management of breeding and pregnancy. Doppler examinations were conducted from fourth week of pregnancy at 10 d interval to evaluate blood flow in uteroplacental artery (UPA), umbilical artery (Uma) and foetal abdominal aorta by measuring pulsatility (PI) and resistive indices (RI). 
Result: Throughout the study the UPA showed biphasic wave. Up to 40 days of gestation the UPA showed diastolic notch and from 41-50 days it disappeared completely. The PI and RI decreased significantly at 5 per cent level between each 10 d interval. Similarly, the PI and RI of Uma decreased significantly at 5 per cent level between each 10 d interval. The PI of foetal abdominal aorta increased significantly at 5 per cent level from £ 30 d to 31-40 d of gestation and thereafter it decreased significantly up to whelping. During early pregnancy the diastolic wave form was absent for Uma and abdominal aorta although at later stages both these vessels showed biphasic wave forms. The study also included the early diagnosis of two abnormal pregnancies, viz., a complete foetal resorption in a Labrador bitch and an incomplete abortion in a Rottweiler bitch by Doppler ultrasound. The incomplete abortion was critically managed and the bitch whelped five healthy puppies fourteen days from the date of the incomplete abortion of three puppies. Thus, the study demonstrated the potential applicability of Doppler Ultrasound in the management of normal and abnormal pregnancies and predicting survival chances of neonates after whelping. 


Critical pregnancy management Doppler ultrasound Maternal/foetal perfusion


  1. Aardema, M.W., Oosterhof, H., Timmer, A., Van Rooy, I. and Aarnoudse, I.G. (2001). Uterine artery Doppler flow and uteroplacental vascular pathology in normal pregnancies and pregnancies complicated by preeclampsia and small for gestational age fetuses. Placenta. 22: 405-411.
  2. Aissi, A. (2008). Aspects of ultrasonographic diagnostics of pregnancy in bitches depending on the first mating. Veterinary World. 1: 293-295.
  3. Aissi, A. and Slimani, C. (2008). Ultrasonographic appearance of the gestational structures throughout pregnancy in bitches. American J. of Anim. and Vet. Sci. 3: 32-35.
  4. Akalin-Sen, T. and Campbell, S. (1992). Understanding the patho-physiology of intra-uterine growth retardation: the role of the ‘lower limb reflex’ in redistribution of blood flow. Eur. J. Obstet. Gynaecol. 192: 937-944
  5. Alvarez-Clau, A. and Liste, F. (2005). Ultrasonographic characterization of the uterine artery in the nonestrus bitch. Ultrasound Med. Biol. 3: 1583-1587. 
  6. Andersen, A.C and Simpson, M.E. (1973). The ovary and the reproductive cycle of the dog (Beagle). Los Altos, CA, USA: Geron-X Inc.
  7. Arduini, D., Rizzo, G. and Romanini, C. (1992). Changes of pulsatility index from foetal vessels preceding the onset of late decelerations in growth-retarded fetuses. Obstet. Gynecol. 79: 605-610.
  8. Barr, F.J. (1988). Pregnancy diagnosis and assessment of fetal viability in the dog - A review. J. Small Anim. Pract. 29: 647-656.
  9. Blanco, P.G., Arias, D., Rube, A., Barrena, J.P., Corrada, Y. and Gobello, C. (2009). An experimental model to study resistance index and systolic/diastolic ratio of uterine arteries in adverse canine pregnancy outcome. Reprod. Domest. Anim. 44: 164-166.
  10. Blanco, P.G., Tórtora, M., Rodríguez, R., Arias, D. and Gobello, C. (2010). Ultrasonographic assessment of maternal cardiac function and peripheral circulation during normal gestation in dogs. Vet. J. 190: 154-159.
  11. Blanco, P.G., Rodríguez, R., Rube, A., Arias, D.O., Tórtora, M., Díaz, J.D. and Gobello, C. (2011). Doppler ultrasonographic assessment of maternal and fetal blood flow in abnormal canine pregnancy. Anim. Reprod. Sci. 126: 130-5.
  12. Chan, F.Y., Lam, C., Lam, Y.H., To, W.K., Pun, T.C. and Lee, C.P. (1994). Umbilical artery Doppler velocimetry compared with fetal heart rate monitoring as a labour admission test. Eur. J. Obstet. Gynaecol. Reprod. Biol. 54: 1-6.
  13. Christie, D.W., Bailey, J.B. and Bell, E.T. (1972). Classification of cell types in vaginal smears during the canine oestrous cycle. Br. Vet. J. 128: 301-310.
  14. Concannon, P.W. (2000). Canine Pregnancy: Predicting Parturition and Timing Events of Gestation [on line]. [Concannon, P.W., England, G., Verstegen, J. and Linde Forsberg, C. (Eds.)]. Available: http://www.ivis.org/docarchive/A1202.0500.pdf[02].
  15. Concannon, P. and Rendano, V. (1983). Radiographic diagnosis of canine pregnancy: onset of fetal skeletal radio-opacity in relation to times of breeding, preovulatory luteinizing hormone release and parturition. Am. J. Vet. Res. 44: 1506-11.
  16. Davidson, A. (1998). Uterine monitoring during pregnancy. In: Proceedings of the annual meeting of the society for Theriogenology, pp. 123-5.
  17. Davidson, A.P. (2003). Approaches to reducing neonatal mortality in dogs. In: Recent Advances in Small Animal Reproduction [on line]. [Concannon, P.W., England, G., Verstegen, J. and Linde-Forsberg, C. (Eds.)], Available: http://www.ivis.org/docarchive/A1226.0303.pdf.
  18. Detti, L., Akiyama, M., Mari, G. (2002). Doppler blood flow in obstetrics. Curr. Opin. Obstet. Gynecol. 14: 587-593.
  19. Di Salvo, P., Bocci, F. and Polisca, A. (2006). Doppler evaluation of maternal and fetal vessels during normal gestation in the bitch. Res. Vet. Sci. 81: 382-388.
  20. England, G.C.W. (1992a). Vaginal cytology and cervicovaginal mucus arborisation in the breeding management of bitches. J. Small Anim. Pract. 33: 577-582.
  21. England, G.C.W. (1992b). Ultrasound evaluation of pregnancy and spontaneous embryonic resorption in the bitch. J. Small Anim. Pract. 33: 430-436.
  22. England, G.C.W. (1998). Ultrasonographic assessment of abnormal pregnancy. Vet. Clin. North Am. Small Anim. Pract. 28. 849-868.
  23. England, G.C.W. and Allen, W.E. (1989). Ultrasound imaging of the reproductive tract of the bitch. Br. J. Radiol. 62: 642.
  24. England, G.C.W. and Allen, W.E. (1990). Studies on canine pregnancy using B-mode ultrasound: Diagnosis of early pregnancy and the number of conceptuses. J. Small Anim. Pract. 31: 321-323.
  25. England G.C.W. and Russo, M. (2006). Ultrasonographic characteristics of early pregnancy failure in bitches. Theriogenology. 66: 1694-1698.
  26. Evans, H.E. (1979). Reproduction and prenatal development. In: Millers’ Anatomy of the Dog. [H.E. Evans, G.C. Christiensen (Eds.)], WB Saunders, Philadelphia. p. 13.
  27. Freitas, d.L.A., Mota, G.L., Silva, H.V.R., Carvalho, C.F., Silva, D.L.D.M. (2016). Can maternal-fetal hemodynamics influence prenatal development in dogs? Anim. Reprod. Sci. 172: 83-93.
  28. Gaikwad, S.M., Gulavane, S.U., Kumbhar, U.B., Shelar, R.R., Chaudhari, R.J. and Ribeiro, R.A. (2020). Doppler evaluation of maternal vessels in normal gestation and threatened abortion in canines. Irish Vet. J. 73: 1-9.
  29. George, J. (2012). Doppler and 3D/4D ultrasonographic studies on development of conceptus in bitch. M.V.Sc. Thesis. LUVAS, Hisar.
  30. Gil, E.M.U., Garcia, D.A.A. Giannico, A.T. and Froes T.R. (2014). Canine fetal heart rate: Do accelerations or decelerations predict the parturition day in bitches? Theriogenology. 82: 933-941.
  31. Gosling, R.G. (1976). Extraction of physiological information from spectrum analysed Doppler-shifted continuous wave ultrasound signals obtained noninvasively from the arterial tree. In: I.E.E. Medical Electronic Monographs. [Hill D.W and Watson B.W. (Eds)] Peter Peregrinus, London, pp. 73-125.
  32. Giannico, A.T., Gil, E.M.U., Garcia, D.A.A., Froes, T.R. (2015). The use of Doppler evaluation of the canine umbilical artery in prediction of delivery time and fetal distress. Animal Reprod. Sci. 154: 105-112.
  33. Jones, D.E., Joshua, J.O. and Morton, D.B. (1988). Pregnancy. In: Reproductive Clinical Problems in the Dog. (Eds.). Wright. London. pp. 33-86, 124, 169-182, 190.
  34. Johnson, C.A. (2008). Pregnancy management in the bitch. Theriogenology. 70: 1412-1417. 
  35. Kelley, R. (2002). Canine reproductive management: Factors affecting litter size. In: Proceedings of the Annual Conference of the Society for Theriogenology and American College of Theriogenology; [Kützler, M.A. (ed.)], 7th to 11th August, 2002, Seattle, WA, USA. American College of Theriogenologists. pp. 291-301.
  36. Linde-Forsberg, C., Holst, B.S. and Govette, G. (1999). Comparison of fertility data from vaginal vs intrauterine insemination of frozen-thawed dog semen: a retrospective study. Theriogenology. 52: 11-23.
  37. Lopate C. (2008). Estimation of gestational age and assessment of canine fetal maturation using radiology and ultrasonography: a review. Theriogenology. 70: 397-402.
  38. Miranda, S.A. and Domingues, S.F. (2010). Conceptus ecobiometry and triplex Doppler ultrasonography of uterine and umbilical arteries for assessment of fetal viability in dogs. Theriogenology. 74: 608-617.
  39. Muller, K. and Arbeiter, K. (1993). Ultrasonographic and clinical signs of fetal resorption in the bitch. J. Reprod. Fertil. Suppl. 47: 558.
  40. Nautrup, C.P. (1998). Doppler ultrasonography of canine maternal and fetal arteries during normal gestation. J. Reprod. Fertil. 112: 301-314.
  41. Nautrup, C.P. and Tobias, C.P. (2000). Testoatlante di ecografia del cane e del gatto. Torino UTET. 2. 5-14.
  42. Nicolaides, K.H., Rizzo, G. and Hecher, K. (2000). Placental and fetal Doppler. The Parthenon Publishing Group inc., New York, pp. 35-66.
  43. Nyland, T.G., Mattoon, J.S. Herrgesell, E.J. and Wisner, E.R. (2002). Physical principles, instrumentation and safety of diagnostic ultrasound. In: Small Animal Diagnostic Ultrasound, [Nyland, T.G., Mattoon, J.S. (Eds.)] (2nd ed.). Philadelphia, W.B. Saunders, pp. 1-18.
  44. Olson, P.N., Husted, P.W., Allen, T.A., Nett, T.M. (1984). Reproductive endocrinology and physiology of the bitch and queen. Vet. Clin. North Am. Small Anim. Pract. 14: 927-946.
  45. Pacheco, A.O., Buenfil, J.C.R., Correa, J.C.S., González, A.R.M.O., Cuello, M.J. (2006). Prevalence of fetal resorption in stray dogs in Yucatan, Mexico. J. Small Anim. Pract. 47. 266-269.
  46. Pineda, M.H. (1989). Reproductive patterns of dogs. In: Veterinary Endocrinology and Reproduction. [McDonald, L.E. and Pineda, M.H. (Eds.)], Lea and Febiger, London. 4th ed. pp. 460-486.
  47. Pourcelot, L. (1974). Applications cliniques de l‘examen Doppler transcutane. Inserm. 34: 213-240.
  48. Reynolds, L.P., Caton, J.S. and Redmer, D.A. (2006). Evidence for altered placental blood flow and vascularity in compromised pregnancies. J. Physiol. 572: 51-58.
  49. Robson, S.C. (2000). Assessment of hemodynamics using Doppler ultrasound. Ultrasound Obstet. Gyneco. 15: 456-459.
  50. Simon, S. (1997). Hormonal induction of oestrus in bitches. M.V.Sc thesis, Kerala Agricultural University, Thrissur, p104.
  51. Simon, S., Nair, P. and Athman, K.V. (1998). Management of infertility in bitches using exfoliative vaginal cytology. J. Vet. Anim. Sci. 29: 50-52.
  52. Simon, S. (2001). Exfoliative vaginal cytology in breeding canines - A case report. Indian Vet. J. 78: 428-429.
  53. Simon, S. (2014). Comparison of anuclear cell index and superficial cell index in cytological evaluation to determine optimum breeding time in bitches. Proceedings of National Symposium on frontier reproduction technologies for enhancing animal fertility and fecundity: global perspective. 8-10 January, 2014, Nagpur, Maharashtra, ISSAR pp. 379
  54. Simon, S. and Gowda, N.B. (2014). Successful whelping of four live pups in a bitch twelve days after abortion of a single foetus. Proceedings of National Symposium on frontier reproduction technologies for enhancing animal fertility and fecundity: global perspective. 8-10 January, 2014, Nagpur, Maharashtra, ISSAR pp. 466.
  55. Simon, S., Ghosh K.N.A. and Appanasamy. R. (2018). Application of Doppler ultrasonographic assessment of maternal and foetal blood flow in canine pregnancy. Abstract Proceedings of 43rd World Small Animal Veterinary Association Congress and 9th FASAVA Congress. 25-28 September, 2018, Singapore. pp. 713. 
  56. Singla, V.K. Varughese, E.E., Upasana, R., Bhat, G.R. and Gandotra, V.K. (2012). Resorption of multiple fetuses in mid gestation in a bitch. Indian J. Anim. Rep. 33: 104-106.
  57. Smith, F.O. (2007). Challenges in small animal parturition-Timing elective and emergency caesarean sections. Theriogenology. 68: 348-353. 
  58. Tønnessen, R., Sverdrup Borgea, K., Nødtvedta, A. and Indrebøa, A. (2012). Canine perinatal mortality: A cohort study of 224 breeds. Theriogenology. 77: 1788-1801.
  59. Verstegen, J.P, Silvia, L.D.M., Onclin, K. and Donnay, I. (1993). Echocardiographic study of heart rate in dog and cat fetuses in utero. J. Reprod. Fertil. Suppl. 47: 17-80.
  60. Yeager, A.E. and Concannon, P.W. (1990a). Association between the preovulatory Luteinizing Hormone surge and the early ultrasonographic detection of pregnant and fetal heart beats in beagle dogs. Theriogenology. 34: 655.
  61. Zone, M.A. and Wanke, M.M. (2001). Diagnosis of foetal health by ultrasonography. J. Reprod. Fertil. Suppl. 57: 215-219.

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