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Indian Journal of Animal Research

Status of Subclinical Mastitis in Crossbred Cattle of Peri-urban Unorganized Herd of Middle Indo-gangetic Plains

DOI: 10.18805/IJAR.B-4241    | Article Id: B-4241 | Page : 1468-1475
Citation :- Status of Subclinical Mastitis in Crossbred Cattle of Peri-urban Unorganized Herd of Middle Indo-gangetic Plains.Indian Journal of Animal Research.2021.(55):1468-1475
Pankaj Kumar, Rashmi Rekha Kumari, Abhay Kumar, Rohan Kumar Raman, P.C. Chandran, Manish Kumar pankajvet@gmail.com
Address : Division of Livestock and Fisheries Management, ICAR-Research Complex for Eastern Region, Patna-800 014, Bihar, India.
Submitted Date : 6-07-2020
Accepted Date : 16-10-2020
First Online:

Abstract

Background: Mastitis is the most common and economically important disease of dairy cattle. Subclinical mastitis is a more important form in India than clinical mastitis. Subclinical mastitis (SCM) detection done by periodic examination of the udder health by evaluation of milk at the herd level or the individual cow level by milk somatic cell count (SCC), followed by culture of random milk samples. The presented study was undertaken by survey and sampling of milk from lactating crossbred cattle of unorganized dairy farms and farmer’s dairy of the peri-urban region of middle Indo-Gangetic Plains. The objective of the study was to monitor the status of SCM in crossbred cattle and associated changes in milk constitutes and oxidative stress in milk. 
Methods: A total of 147 lactating crossbred cattle were screened for SCM using the modified California Mastitis Test (CMT) using detergent based CMT reagent and compared with conventional CMT reagent, followed by SCC, milk constituents, bacterial isolation and antibiotic sensitivity testing (ABST). General information was collected in pre-tested questionnaire. The data obtained were statistically treated to evaluate significance of the study.
Conclusion: The overall prevalence of subclinical mastitis in peri-urban cross-bred cattle was 36.74% which varied with parity and stage of milking. Prevalence of subclinical mastitis was highest (55.77%) in cross-bred cattle in mid-lactation and Parity 3-5. The mean SCC was significantly higher (7.21±0.27) in subclinical mastitis compared to CMT negative (3.66±0.06) milk sample. Somatic cell count was positively and significantly correlated with CMT reactions using conventional CMT reagent (rs= 0.86) as well as modified CMT reagent (rs=0.815) attempted using Spearman rank-order correlation coefficient. The mean values of milk pH, fat and lactic acid acidity increased significantly (P£0.01) in SCM compared to the milk of healthy cattle, except lactose which decreased significantly in SCM milk. The common contagious bacteria responsible for SCM isolate were coagulase-positive Staphylococcus spp. (64.82%) isolates from these SCM milk followed by Streptococcus spp. ABST test conducted on random CMT positive milk sample indicated that gentamicin as most sensitive, followed by enrofloxacin. The present finding indicates the suitability of enrofloxacin as the most useful antibiotic for the treatment of subclinical mastitis in cross-bred cattle of the peri-urban area of middle Indo-Gangetic plains.

Keywords

ABST Cross-bred cattle Detergent based CMT reagent Milk constituents Screening Sub-clinical mastitis

References

  1. Atakisi, O., Oral, H., Atakisi, E., Merhan, O., Pancarci, S.M., Ozcan, A., Marasli, S., Polat, B., Colak, A. and Kaya, S. (2010). Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science. 89(1): 10-13.
  2. Bhoite, U.Y. and Padekar, R.N. (2002). Factors affecting milk yield and composition of Gir halfbreds. Indian Journal of Animal Research. 36(1): 67-69.
  3. Bureau of Indian Standards. (1960). In: Method of tests for dairy industry. Part I. Rapid examination of milk. Bureau of Indian Standards, New Delhi, 1469.
  4. Cowan, S.T. and Steel, K.J. (1965). Manual for the identification of medical bacteria. Manual for the Identification of Medical Bacteria.
  5. Dimri, U., Sharma, M.C., Singh, S.K., Kumar, P., Jhambh, R., Singh, B., Bandhyopadhyay, S. and Verma, M.R. (2013). Amelioration of altered oxidant/antioxidant balance of Indian water buffaloes with subclinical mastitis by vitamins A, D 3, E and H supplementation. Tropical Animal Health and Production. 45(4): 971-978.
  6. Dohoo, I.R. and Meek, A.H. (1982). Somatic cell counts in bovine milk. The Canadian Veterinary Journal. 23(4): 119.
  7. Dürr, J.W., Cue, R.I., Monardes, H.G., Moro-Méndez, J. and Wade, K.M. (2008). Milk losses associated with somatic cell counts per breed, parity and stage of lactation in Canadian dairy cattle. Livestock Science. 117: 225-232.
  8. Elango, A., Doraisamy, K.A., Rajarajan, G.and Kumaresan, G. (2010). Bacteriology of sub clinical mastitis and antibiogram of isolates recovered from cross bred cows. Indian Journal of Animal Research. 44(4): 280-284.
  9. Erel, O. (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical biochemistry. 37(2): 112-119.
  10. Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry. 38(12):1103-1111.
  11. Ghosh, B. and Prasad, J. (1998). Milk yield and composition as influenced by udder measurements, in Jersey × Red Sindhi crosses. Indian Journal of Animal Production & Management. 14: 23-25.
  12. Hardenberg, F. (2016). Clinical and subclinical mastitis in dairy cattle and buffaloes in Bihar, India: Prevalence, major pathogens and risk factors. Uppsala, Sweden: Swedish University of Agricultural Sciences.
  13. Hegde, R., Isloor, S., Prabhu, K.N., Shome, B.R., Rathnamma, D., Suryanarayana, V.V.S., Yatiraj, S., Prasad, C.R., Krishnaveni, N., Sundareshan, S. and Akhila, D.S. (2013). Incidence of subclinical mastitis and prevalence of major mastitis pathogens in organized farms and unorganized sectors. Indian Journal of Microbiology. 53(3): 315-320.
  14. Hiitiö, H., Vakkamäki, J., Simojoki, H., Autio, T., Junnila, J., Pelkonen, S. and Pyörälä, S. (2017). Prevalence of subclinical mastitis in Finnish dairy cows: changes during recent decades and impact of cow and herd factors. Acta Veterinaria Scandinavica, 59(1): 22.
  15. Jena, B., Pagrut, N.K., Sahoo, A. and Ahmed, A. (2015). Subclinical bovine mastitis in rural, peri-urban and suburban regions of Jaipur district of Rajasthan, India. Journal of Animal Research. 5(1): 175-182.
  16. Jin, L., Yan, S., Shi, B., Bao, H., Gong, J., Guo, X. and Li, J. (2014). Effects of vitamin A on the milk performance, antioxidant functions and immune functions of dairy cows. Animal Feed Science and Technology. 192: 15-23.
  17. Jingar, S.C., Mehla, R.K., Singh, M., Kumar, A., Kantwa, S.C. and Singh, N. (2014). Comparative study on the incidence of mastitis during different parities in cows and buffaloes. Indian Journal of Animal Research. 48(2): 194-197.
  18. Joshi, S. and Gokhale, S. (2006). Status of mastitis as an emerging disease in improved Periurban dairy farms in India. Annals of the New York Academy of Sciences. 1081: 74-83.
  19. Kumar, M., Singh, N.K. and Choudhary, R.K. (2012). Study of intrinsic factors responsible for prevalence of bovine mastitis in the peri-urban areas of Patna. Journal of Interacademicia. 16: 411-415.
  20. Martins, T., Santos, A.F.S., Miranda, M.S., Motta, T.P., Ambrósio, L.A., Pozzi, C.R. and Arcaro, J.R. (2014). Persistence of gentamicin residues in cow milk after intramammary treatment. Revue de Médecine Vétérinaire. 165: 62-67.
  21. McHugh, M.L. (2012). Interrater reliability: the kappa statistic. Biochemia medica. 22(3): 276-282.
  22. Miekley, B., Traulsen, I. and Krieter, J. (2013). Principal component analysis for the early detection of mastitis and lameness in dairy cows. Journal of Dairy Research. 80(3): 335-343.
  23. Modh, R.H., Islam, M.M., Patel, Y.G., Modi, R.J. and Wadhwani, K.N. (2017). Effect of parity on udder and teat biometry and its association with milk yield in Gir cows. International Journal of Environmental Science and Technology. 6: 2068-2073.
  24. Mubarack, H.M., Doss, A., Vijayasanthi, M. and Venkataswamy, R. (2012). Antimicrobial Drug Susceptibility of Staphylococcus aureus from Subclinical Bovine Mastitis in Coimbatore, Tamilnadu, South India, Veterinay World. 5(6): 352-355.
  25. Mukherjee, J. and Dang, A.K. (2011). Immune activity of milk leukocytes during early lactation period in high and low yielding crossbred cows. Milchwissenschaft-Milk Science International. 66(4): 384-388.
  26. Paixão, M.G., Abreu, L.R., Richert, R. and Ruegg, P.L. (2017). Milk composition and health status from mammary gland quarters adjacent to glands affected with naturally occurring clinical mastitis. Journal of Dairy Science. 100(9): 7522-7533.
  27. Pankaj, Sharma, A., Chhabra, R. and Sindhu, N. (2012). Prevalence of sub clinical mastitis in cows: Its etiology and antibiogram. Indian Journal of Animal Research. 46(4): 348-353.
  28. Pati, B.K. and Mukherjee, R. (2016). Characterization of Staphylococcus aureus Isolates of Bovine Mastitis Origin and Antibiotic Sensitivity Pattern from Northern Plains of India. Journal of Veterinary Research Animal Husbandry. 1(1): 105.
  29. Pitkala, A., Haveri, M., Pyorala, S., Myllys, V. and Honkanen-Buzalski, T. (2004). Bovine mastitis in Finland 2001- Prevalence, distribution of bacteria and antimicrobial resistance. Journal of Dairy Science. 87: 2433-2441.
  30. Quinn, P.J., Markey, B.K., Leonard, F.C, Hartigan, P., Fanning S. and Fitzpatrick, E.S. (2011). In: Veterinary Microbiology and Microbial Disease. New Jersey, U.S.A: Wiley-Blackwell, Hoboken.
  31. Radhika, G., Ajithkumar, S., Rani, A., Sathian, C.T., Anilkumar, K., Usha, A.P. and Dinesh, C.N. (2012). Milk yield and composition of crossbred cows in the hilly Wayanad district of Kerala, India. Indian Journal of Animal Sciences. 82: 1251-1254. 
  32. Ranjan, Abhishek (2017). The unsuitable boy of India’s cattle economy. Village Square, epublication.https://www.villagesquare.in/2017/06/28/unsuitable-boy-indias-cattle-economy/.
  33. Sarkar, U., Gupta, A.K., Sarkar, V., Mohanty, T.K., Raina, V.S. and Prasad, S. (2006). Factors affecting test day milk yield and milk composition in dairy animals. Journal of Dairying, Foods and Home Science. 25: 129-132.
  34. Saravanan, R., Das, D.N., De, S. and Panneerselvam, S. (2015). Effect of season and parity on somatic cell count across zebu and crossbred cattle population. Indian Journal of Animal Research. 49(3): 383-387.
  35. Schalm, O.W., Carrol, J.E. and Jain, N.C. (1971). Bovine Mastitis. 1st Ed., Lea and Febiger, Philadelphia, USA. pp: 132-153.
  36. Schmidt, T., Kock, M.M. and Ehlers, M.M. (2015). Diversity and antimicrobial susceptibility profiling of staphylococci isolated from bovine mastitis cases and close human contacts. Journal of Dairy Science. 98(9): 6256-6269.
  37. Schneider, R. and Jasper, D.E. (1964). Standardization of the California Mastitis Test. American Journal of Veterinary Research. 25: 1635.
  38. Singh, M. and Dang, A.K. (2002). Somatic cell count of milk. Technical Book. Karnal, India: Published by National Dairy Research Institute. pp. 1-25.
  39. Snedecor G.W. and Cochran, W.G. (1994) Statistical Methods. 8th ed., Iowa State University Press, Ames, Iowa.
  40. Sordillo, L.M. and Aitken, S.L. (2009) Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary immunology and immunopathology. 128(1-3): 104-109.
  41. Sreedhar, S. and Sreenivas, D. (2015). A study on calf mortality and managemental practices in commercial dairy farms. Livestock Research International. 3(4): 94-98. 
  42. Tiwari, R., Sharma, M.C. and Singh, B.P. (2007). Buffalo health care in commercial dairy farms: A field study in Uttar Pradesh (India). Livestock Research for Rural Development. 19(3): 62-64.
  43. Turk, R., Koledić, M., Maćešić, N., Benić, M., Dobranić, V., Đuričić, D., Cvetnić, L. and Samardžija, M. (2017). The role of oxidative stress and inflammatory response in the pathogenesis of mastitis in dairy cows. Mljekarstvo. 67: 91-101.
  44. Weiss, W.P., Hogan, J.S. and Smith, K.L. (2004). Changes in vitamin C concentrations in plasma and milk from dairy cows after an intramammary infusion of Escherichia coli. Journal of Dairy Science. 87(1): 32-37.
  45. Zecconi, A., Sesana, G., Vairani, D., Cipolla, M., Rizzi, N. and Zanini, L. (2019). Somatic cell count as a decision tool for selective dry cow therapy in Italy. Italian Journal of Animal Science. 18(1): 435-440.

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