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An Alternative Buffalo Urine-based Non-invasive Early Estrus Test using Wheat and Mung Bean Seed Germination

DOI: 10.18805/IJAR.B-4237    | Article Id: B-4237 | Page : 1279-1285
Citation :- An Alternative Buffalo Urine-based Non-invasive Early Estrus Test using Wheat and Mung Bean Seed Germination.Indian Journal of Animal Research.2021.(55):1279-1285
Tanvi Bhatia, Varij Nayan, Rakshita Singh, Chhama Singh, Anuradha Bhardwaj, Sunil Kumar, M. Naveen Swaroop, S.K. Onteru, R.K. Sharma, Anurag Bharadwaj, Dheer Singh, A.K. Mohanty varij.biochem@gmail.com
Address : Division of Animal Physiology and Reproduction, Molecular Endocrinology, Functional Genomics and Computational Biology Laboratory, ICAR-Central Institute for Research on Buffaloes, Hisar-125 001, Haryana, India.
Submitted Date : 1-07-2020
Accepted Date : 29-10-2020

Abstract

Background: The silent estrus problem in buffaloes is one of the major bottlenecks in buffalo management. Here, we present for the first time a simple and urine-based non-invasive seed germination inhibition test to detect the early estrus in buffaloes.
Methods: The urine samples were collected from buffaloes on 0, 6, 10, 14, 18, 21 and 28 days after detection of signs of heat. The mungbean and wheat seeds (n=15 each) were treated with diluted (1:2 and 1:4) and undiluted urine samples. The results were analyzed in terms of germination inhibition percentage (GI%) and shoot length (SL) measurements. Control was established using distilled water in place of urine.
Conclusion: In 1:4 dilution urine samples, a significant (P ≤ 0.05) decrease in GI% and SL were observed after 48 hours and 5 days, respectively in both the seeds, which could be useful as an estrus test. No significant results were observed for GI% and SL with undiluted and 1:2 diluted samples in both seeds. Our findings demonstrate that GI% and SL were inhibited around peri-estrus events, increased from 10th-14th days and then gradually decreased with 1:4 dilution of urine. This estrus test may assist the farmers in timing AI.

Keywords

Estrus Germination inhibition percentage Murrah buffaloes Seed germination test Urine

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