Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Indian Journal of Animal Research, volume 55 issue 11 (november 2021) : 1279-1285

An Alternative Buffalo Urine-based Non-invasive Early Estrus Test using Wheat and Mung Bean Seed Germination

Tanvi Bhatia, Varij Nayan, Rakshita Singh, Chhama Singh, Anuradha Bhardwaj, Sunil Kumar, M. Naveen Swaroop, S.K. Onteru, R.K. Sharma, Anurag Bharadwaj, Dheer Singh, A.K. Mohanty
1Division of Animal Physiology and Reproduction, Molecular Endocrinology, Functional Genomics and Computational Biology Laboratory, ICAR-Central Institute for Research on Buffaloes, Hisar-125 001, Haryana, India.
Cite article:- Bhatia Tanvi, Nayan Varij, Singh Rakshita, Singh Chhama, Bhardwaj Anuradha, Kumar Sunil, Swaroop Naveen M., Onteru S.K., Sharma R.K., Bharadwaj Anurag, Singh Dheer, Mohanty A.K. (2021). An Alternative Buffalo Urine-based Non-invasive Early Estrus Test using Wheat and Mung Bean Seed Germination. Indian Journal of Animal Research. 55(11): 1279-1285. doi: 10.18805/IJAR.B-4237.
Background: The silent estrus problem in buffaloes is one of the major bottlenecks in buffalo management. Here, we present for the first time a simple and urine-based non-invasive seed germination inhibition test to detect the early estrus in buffaloes.
Methods: The urine samples were collected from buffaloes on 0, 6, 10, 14, 18, 21 and 28 days after detection of signs of heat. The mungbean and wheat seeds (n=15 each) were treated with diluted (1:2 and 1:4) and undiluted urine samples. The results were analyzed in terms of germination inhibition percentage (GI%) and shoot length (SL) measurements. Control was established using distilled water in place of urine.
Conclusion: In 1:4 dilution urine samples, a significant (P ≤ 0.05) decrease in GI% and SL were observed after 48 hours and 5 days, respectively in both the seeds, which could be useful as an estrus test. No significant results were observed for GI% and SL with undiluted and 1:2 diluted samples in both seeds. Our findings demonstrate that GI% and SL were inhibited around peri-estrus events, increased from 10th-14th days and then gradually decreased with 1:4 dilution of urine. This estrus test may assist the farmers in timing AI.

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