Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 9 (september 2021) : 1044-1048

Leptin Supplementation Stimulates Synergism with Growth Factors and Hormones to Express Its Receptor in Cultured Preantral Follicles of Sheep

K. Sravani Pragna, V. Praveen Chakravarthi, Deepa Pathipati, B. Rambabu Naik, L.S.S. Varaprasad Reddy, B. Punya Kumari, A.V.N. Siva Kumar
1Department of Veterinary Physiology, Sri Venkateswara Veterinary University, Tirupati-517 502, Andhra Pradesh, India. 
Cite article:- Pragna Sravani K., Chakravarthi Praveen V., Pathipati Deepa, Naik Rambabu B., Reddy Varaprasad L.S.S., Kumari Punya B., Kumar Siva A.V.N. (2021). Leptin Supplementation Stimulates Synergism with Growth Factors and Hormones to Express Its Receptor in Cultured Preantral Follicles of Sheep. Indian Journal of Animal Research. 55(9): 1044-1048. doi: 10.18805/IJAR.B-4218.
Background: Leptin receptor is a transmembrane receptor that regulates reproduction at molecular level.Since for action of any hormone on target cell and to have local action on any tissue, expression of its own receptor is necessary and also it is not known whether such improvement in ovarian follicular development by Leptin is mediated through presence of its homologous receptors in the sheep ovaries. Therefore this study aimed on expression of Leptin receptor mRNA in cultured ovarian follicles of sheep by RT PCR.
Methods: Leptin receptor mRNA expression in sheep was studied using qRT-PCR from: (i) In vivo grown preantral, early antral, antral, large antral follicles and cumulus oocyte complexes obtained from large antral follicles subjected to 24h of in vitro maturation and (ii) PFs’ exposed to three different culture media for 3min, two, four or six days and subsequently matured in vitro for 24h.  
Result: Leptin receptor was observed at all stages ovarian follicles in both cumulus cells and oocytes. Leptin supplementation along with other growth factors and hormones stimulated the expression of its receptor mRNA which is parallel to in vivo stages which could suggest synergistic action of growth factors and hormones with Leptin. 
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