Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 55 issue 9 (september 2021) : 1018-1026

Comparative Homology Modeling and Physicochemical Characterization of Cyprinus carpio Hsp70 Protein

Pravas Ranjan Sahoo, Santosini Sahu, Geetanjali Das, Gyanaranjan Sahoo, Prakash Chandra Behera
1Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar-7510 03, Odisha, India.
Cite article:- Sahoo Ranjan Pravas, Sahu Santosini, Das Geetanjali, Sahoo Gyanaranjan, Behera Chandra Prakash (2021). Comparative Homology Modeling and Physicochemical Characterization of Cyprinus carpio Hsp70 Protein. Indian Journal of Animal Research. 55(9): 1018-1026. doi: 10.18805/IJAR.B-4215.

ABSTRACT

Background: Expression of the heat shock proteins (Hsp) is responsible for the protection from adverse climatic changes particularly heat stress in Common carp (Cyprinus carpio). Although, with advancement of molecular techniques, Hsp70 protein has been isolated but this protein needs to be characterized by both physicochemically and structurally for the functional annotation of fish genome. So this current study was undertaken with aim of generation of various protein models and also for thorough physiochemical characterization of this protein.
Methods: In this study, Hsp70 protein of common carp was characterized by both physiochemical and structurally through insilco platform and as the crystal structure of this protein is not available, protein models were created though homology modelling upon Modeller version 9.21, Phyre2 and Swiss-model and then the generated predicted models were evaluated through Rampage, Errat, Verify 3D, ProQ and ProSA analysis.
Result: Our investigation showed that this protein is very stable, hydrophilic with no disulphide bonds and the protein models which were generated from this study, are of good quality with z value of - 9.58, -9.48 and -10.93 and quality factor of 82.56, 59.43 and 95.27 respectively. So this study was concluded that the generated Hsp70 protein models would provide an avenue for the other researchers for development of high-throughput gene function assignment in fish.

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