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Studies on the Efficacy of Single and Twice Application of Mesenchymal Stem Cells in Full Thickness Cutaneous Wound Healing

DOI: 10.18805/IJAR.B-4212    | Article Id: B-4212 | Page : 1330-1336
Citation :- Studies on the Efficacy of Single and Twice Application of Mesenchymal Stem Cells in Full Thickness Cutaneous Wound Healing.Indian Journal of Animal Research.2021.(55):1330-1336
Jyotsana Bhatt, Amarpal, Abas Rashid Bhat, Anuj Pratap Singh, A.M. Pawde, Irfan Ahmed Bhat, G. Taru Sharma bhattjyotsana24@gmail.com
Address : Division of Surgery, Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh, India.
Submitted Date : 6-06-2020
Accepted Date : 21-10-2020

Abstract

Background: A lot of research has been done in the field of wound healing utilising stem cell therapy. This study was conducted to compare the efficacy of single and twice application of mesenchymal stem cells for cutaneous wound healing.
Methods: The present study was done on 18 guinea pigs and a 2.5 X 2.5 cm2 full-thickness skin wound was created on the dorsum of each under standard anesthetic protocol. Animals were divided into 3 groups having 6 animals in each. The animals of group I  were administered phosphate buffer saline (PBS) subcutaneously on day 0, group II received 1×106 mesenchymal stem cells (MSCs) in PBS subcutaneously on day 0 and group III received 1×106 MSCs in PBS subcutaneously on day 0 and again at day 3, at wound margins. Clinical, photographic, histopathological and histochemical parameters were recorded for each animal.
Result: An overall gross and histopathological evaluation suggested early wound closer, better tissue granulation, early orientation of fibrocytes and collagen fibers, revealing a superior quality healing in animals of group III as compared to rest of the groups. From the above study it was concluded that twice application of MSCs leads to faster and qualitatively better healing. 

Keywords

Guinea pigs Histopathology Mesenchymal stem cells Wound healing

References

  1. Aggarwal, S. and Pittenger, M.F. (2005). Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 105: 1815-1822.
  2. Amarpal, Kinjavdekar, P., Aithal, H.P., Pawde, A.M., Singh, J. and Udheiya, R. (2010). Evaluation of xylazine, acepromazine and medetomidine with ketamine for general anaesthesia in rabbits. Scandinavian Journal of Laboratory Animal Science. 37: 223-229.
  3. Ansari, M.M., Sreekumar, T.R., Chandra, V., Dubey, P.K., Kumar, G.S., Amarpal, Sharma, G.T. (2013). Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing. The Journal of Stem Cell Research and Therapy. 3: 141.
  4. Borena, B.M., Pawde, A.M., Amarpal, Aithal, H.P., Kinjavdekar, P., Singh, R. and Kumar, D. (2010). Evaluation of autologous bone marrow-derived nucleated cells for healing of full-thickness skin wounds in rabbits. International Wound Journal. 7(4): 249-260.
  5. Dulchavsky, D., Gao, X., Liu, Y.B., Deeb, D., Arbab, A.S., McIntosh, K., Dulchavsky, S.A. and Gautam, S.C. (2008). Bone marrow-derived stromal cells (BMSCs) interact with ûbroblasts in accelerating wound healing. Journal of Investigative Surgery. 21: 270-279.
  6. Falanga, V. (2005). Wound healing and its impairment in the diabetic foot. The Lancet. 366(9498): 1736-1743.
  7. Gade, N.E., Pratheesh, M.D., Nath, A., Dubey, P.K., Sharma, B., Saikumar, G. and Taru Sharma, G. (2013). Molecular and Cellular Characterization of Buffalo Bone Marrow-Derived Mesenchymal Stem Cells. Reproduction in Domestic Animals. 48(3): 358-367.
  8. Gnecchi, M. and Melo, L.G. (2009). Bone marrow-derived mesenchymal stem cells: isolation, expansion, characterization, viral transduction and production of conditioned medium. Stem Cells in Regenerative Medicine. 281-294.
  9. Gnecchi, M., Zhang, Z., Ni, Dzau, V.J. (2008). Paracrine mechanisms in adult stem cell signaling and therapy. Circulation Research. 103: 1204-1219.
  10. Gurtner, G.C., Werner, S., Barrandon, Y. and Longaker, M.T. (2008). Wound repair and regeneration. Nature. 453: 314-321.
  11. Kinnaird, T., Stabile, E., Burnett, M.S., Shou, M. and Lee, C.W. (2004). Local delivery of marrow-derived stromal cells augments collateral perfusion through paracrine mechanisms. Circulation. 109: 1543-1549.
  12. Lau, R., Paus, Tiede, S., Day, P. and Bayat, A. (2009). Exploring the role of stem cells in cutaneous wound healing. Experimental Dermatology. 18: 921-933.
  13. Lillie, R.D. (1948). Histological Technic., Blakiston Co., Philadelphia: 196.
  14. Mallory, F.B. (1942). Bacteriological examinations. In: Pathological technique: A practical manual for the pathological laboratory. W.B. Saunders, Philadelphia: 170-171.
  15. Martin, P. and Nunan, R. (2015). Cellular and molecular mechanisms of repair in acute and chronic wound healing. British Journal of Dermatology. 173(2): 370-378.
  16. Masson, P.J. (1929). Some histological methods: trichome stainings and their preliminary techniques. Journal of Technical  Methods. 12: 75-90.
  17. Maxson, S., Lopez, E.A., Yoo, D., Danilkovitch-Miagkova, A., LeRoux, M.A. (2012). Concise review: role of mesenchymal stem cells in wound repair. Stem Cells Translational Medicine. 1(2): 142-149.
  18. Murphy, M.B., Moncivais, K. and Caplan, A.I. (2013). Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine. Experimental and Molecular Medicine. 45(11): 54-59.
  19. Nakagawa, H., Akita, S. and Fukui, M. (2005). Human mesenchymal stem cells successfully improve skin-substitute wound healing. British Journal of Dermatology. 153: 29-36.
  20. Nuschke, A. (2014). Activity of mesenchymal stem cells in therapies for chronic skin wound healing. Organogenesis. 10(1): 29-37. 
  21. Pratheesh, M.D., Dubey, P.K., Gade,N.E., Nath, A., Sivanarayanan, T.B., Madhu, D.N., Somal, A., Baiju, I., Sreekumar, T.R., Gleeja, V.L., Bhat, I.A., Chandra, V., Amarpal, Sharma, B., Saikumar, G., Sharma, G.T. (2017). Comparative study on characterization and wound healing potential of goat (Capra hircus) mesenchymal stem cells derived from fetal origin amniotic fluid and adult bone marrow. Research in Veterinary Science. 112: 81-88.
  22. Salgado, A., Reis, R., Sousa, N. and Gimble, J. (2010). Adipose tissue derived stem cells secretome: soluble factors and their roles in regenerative medicine. Current Stem cell Research and Therapy. 5(2): 103-110.
  23. Siegel, S. and Castellan, N.J. (1988). Non parametric statistics for behavioural sciences. 2nd Ed. Mc Graw Hill, Singapore.
  24. Smith, A.N., Willis, E., Chan, V.T. et al. (2010). Mesenchymal stem cells induce dermal fibroblast responses to injury. Experimental Cell Research. 316: 48-54.
  25. Smith, M.J., Haltom, J.D. and Gainor, B.J. (2008). Effects of anticoagulation on wound healing using a tensile test. Orthopaedics. 31: 373.
  26. Udehiya, R.K., Amarpal, Kinjavdekar, P., Aithal, H.P., Nath, A., Pawde, A.M. and Sharma, G.T. (2013). Isolation, ex-vivo expansion and characterization of rabbit bone marrow derived mesenchymal stem cells (rBM-MSCs). Indian Journal of Veterinary Surgery. 34: 41-46. 
  27. Wan, J., Xia, L., Liang, W., Liu, Y. and Cai, Q. (2013). Transplantation of bone marrow-derived mesenchymal stem cells promotes delayed wound healing in diabetic rats. Journal of Diabetes Research. 2013.

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