Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.44

  • SJR .282 (2022)

  • Impact Factor .427 (2022)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Experimentally Induced Hyperlipidemia and Associated Atherosclerosis in the Aorta and Vascular Expression of CD 31, CD44, Beta-catenin and E Cadherin in Wistar Male Rats

H. Srinivasa Naik, Ch. Srilatha, K. Sujatha, B. Sreedevi, Prasad TNVKV
  • Email
1Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517 502, Andhra Pradesh, India.
Cite article:- Naik Srinivasa H., Srilatha Ch., Sujatha K., Sreedevi B., TNVKV Prasad (2022). Experimentally Induced Hyperlipidemia and Associated Atherosclerosis in the Aorta and Vascular Expression of CD 31, CD44, Beta-catenin and E Cadherin in Wistar Male Rats. Indian Journal of Animal Research. 56(9): 1144-1148. doi: 10.18805/IJAR.B-4209.

ABSTRACT

Background: Hyperlipidemia is the disorder of lipid metabolism, characterized by elevated serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and very low-density lipoproteins cholesterol (VLDL-C) and decreased high-density lipoprotein cholesterol (HDL-C). Atherosclerosis is a cardiovascular and fibroproliferative inflammatory disease commonly associated with dyslipidemia.
Methods: The study was conducted using 24 Wistar male rats, divided into two groups of 12 rats each. Hyperlipidemia and atherosclerosis were induced by the addition of 1% cholesterol and 15% saturated oil to the 1000g of standard rat diet and given to group II rats. Group I kept as control and maintained for 90 days. 
Result: The present experiment established hyperlipidemia with a significant increase of TC, TG, LDL-C and decreased HDL-C cholesterol with initiated atherosclerotic lesions in the aorta and showed varying degrees of positivity with CD31, CD44, Cadherin and Beta-catenin proteins.

REFERENCES

  1. Alexender, J.S., Blaschuk, O.W., Haselton, F.R. (1993). An N-cadherin like protein contributes to solute barrier maintenance in cultured endothelium. J. Cell. Physiol. 156: 610-618. 
  2. Angst, B.D., Marcozzi, C., Magee, A.I. (2001). The cadherin superfamily: diversity in form and function. J. Cell. Sci. 114: 629-641. 
  3. Attia, D.M., Boer, P., Attia, M.A., Goldschmeding, R., Koomans, H.A., Vaziri, N.D., Joles, J.A. (2002). Proteinuria is preceded by decreased nitric oxide synthesis and prevented by a NO donor in cholesterol-fat rats. Kidney Int. 61: 1776-1787.
  4. Aurelie, B., Anne, N.S., Sylvia, H., Marie, H.G., Jean, C.T., Robert, S., Francoise, M.S. (2008). E cadherin/B-catenin/T-cell factor pathway is involved in smooth muscle cell proliferation elicited by Oxidized low density lipoprotein. Circular Res 10.1161: 694-701. 
  5. Balakumar, P., Jindal, S., Shah, D.I., Singh, M. (2007). Experimental models for vascular endothelial dysfunction. Trends Med. Res. 2: 12- 20.
  6. Coleman, R., Hayek, T., Keidar, S., Aviram, M. (2006). A mouse model for human atherosclerosis: long-term histopathological study of lesion development in the aortic arch of apolipo protein E-deficient (E0) mice. Acta Histochem. 108(6): 415-24.
  7. Coles, E.H. (1986). Veterinary clinical pathology, WB Saunders Company, Philadephia, USA 445-446.
  8. Culling, C.F.A. (1974). Hand Book of Histopathological and Histochemical Techniques (Including Museum Techniques) 3rd edn.
  9. Elena, G., Klaus, L. (2007). Vascular Adhesion melecules in atherosclerosis. Arterioscler Thromb. Vasc. Biol. 27: 2104-2112. 
  10. Faheemuddin, M.D., Janarthan, M., Durraivel, S. (2013). Evaluation of protective effect of cleome viscosa extract on diet induced atherosclerosis in diabetic rats. Journal of chemical and pharmaceutical sciences. 6(4): 238-242.
  11. Frederick, J.R. (2009). Hypercholesterolemia in children and young adults-Current management. JEMDSA.14: 9-12. 
  12. Goel, R., Schrank, B.R., Arora, S., Boylan, B., Fleming, B., Miura, H., Newman, P.J., Molthen, R.C., Newman, P.J., Molthen, R.C., Newman, D.K. (2008). Site specific effects of PECAM-1 on atherosclerosis in LDL recptor deficient mice, Arteroscler Thromb. Vasc. Biol. 28: 1996-2002. 
  13. Harini Devi, N., Ambika Devi, K., Prabath kumar, D., Vani, N. (2016). Lipid ratios, atherogenic coefficient and atherogenic index of plasma as parameters in assessing cardiovascular risk in type 2 diabeties mellitus. Int. J. Res. Med. Sci. 4(7): 2863-2869.
  14. Harry, B.L., Sanders, J.M., Feaver, R.E., Lansey, M., Deem, T.L., Zarbock, A., Bruce, A.C., Pryor, A.W., Gelfand, B.D., Blackman, B.R., Schwartz, M.A., Ley, K. (2008). Endothelial cell PECAM-1 promotes atherosclerotic lesions in areas of disturbed flow in ApoE-deficient mice. Arterioscler. Thromb. Vasc. Biol. 28: 2003-2008. 
  15. Hoschuetzky, H., Aberle, H., Kemler, R. (1994). Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor. J. Cell. Biol 127: 1375-1380. 
  16. Huang, Z.S., Chien, K.L., Yang, C.Y., Tsai, K.S., Wang, C.H. (2001). Peripheral differential leukocyte counts in humans vary with hyperlipidemia, smoking and body mass index. Lipids. 36: 237-245.
  17. Ivanov, D, Philppova M, Antropova J, Gubaeva F, Ijinskaya O, Tararak E, Bochkov. V, Erne P, Resink T, Tkachuk V. (2001). Expression of cell adhesion molecule T-cadherin in the human vasculature. Histchem. Cell. Biol 115: 231-242. 
  18. Jain, N.C. (1986). Schalms Veterinary Hematology - Lea and Febiger Philadephia II edition.
  19. Lampugnani, M.G., Resnati, M., Raiteri, M., Pigott, R., Piscane, A., Houen, G., Ruco, L.P., Dejana, E. (1992). A novel endothelial specific membrane protein is a marker of cell-    cell contacts. J. Cell. Biol. 118: 1511-1522. 
  20. Mohamed Anwar K., Abdelhalim and Hisham A. Alhadlaq (2008). Effect of cholesterol feeding periods on blood haematology and biochemistry of rabbits, International Journal of Biological Chemistry. 2(2): 49-53.
  21. Naiel Abbass K., Alkhafaji, Abdul Razzak A. and Latif (2012). Effects of green tea extract on prevention and treatment of Dyslipidemia in cholesterol-fed male rabbits. Kufa Med. Journal. 2012. 15(1): 175-182.
  22. Newman, P.J., Newman, D.K. (2003). Signal transduction pathway mediated by PECAM-1: new role for an old molecule in platelet and vascular cell biology. Arterioscler. Thromb. Vasc. Biol 23: 953-964.
  23. Olubukola, S.O., Graeme, B., Anthony, J.A. (2012). Protective effect of Tulbaghia violacea Harv.on arotic pathology, tissue antioxidant enzymes and liver damage in diet induced atherosclerosis. Int. J. Mol. Sci. 13: 12747-12760.
  24. Protasiewicz, J., Adamiec, R. (2005). The role of CD44 in pathogenesis of atherosclerosis. Pol Merku Lekarski. 19(112): 559-62. 
  25. Rahaman, M.A., Durrai, vel., Janardhan, Pragathi, K.N., Deep, R. (2013). Evaluation of the Anti hyperlidimeic and Anti atherosclerotic activities of Ethanolic extract of Cissus pallida in atherogenic diet fed rat. Int. J. Phar. Res. Sch. 2: 1-3.
  26. Sjoberg, S. (2007). A role of CD44 in atherosclerosis? Studies in mice and humans, Atherosclerosis 190: 291-297.
  27. Snedecor, W.G., Cochran, G.W. (1994). Statistical Methods, 6th Edn. Oxford and IBH Publishing Company, New Delhi.
  28. Stary Herbert, C.A., Bleakley, C., Robert Dinsmore, E., Valentin, F., Seymour, G., William, I., Michael Rosenfeld, E., Colin, J., Schwartz, William Wagner, D., Robert Wissler, W. (1995). A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis. Circulation. 92: 1355-134.
  29. Woodfin, A., Voisin, M.B., Nousharaghm S. (2007). PECAM-1: a multifunctional molecule in inflammation and vascular biology. Arterioscler. Thromb. Vasc. Biol 27: 2514-2523. 
  30. Wyder, L., Vitaliti, A., Scheneider, H., Hebbard, L.W., Moritz, D.R., Wittmer, M., Ajmo, M., Klemenz, R. (2000). Increased expression of H/T-cadherin in tumor penetrating blood vessels. Cancer. Res. 60: 4682-4688.
  31. Yuri, B.V., Reginald, L.S.A., Teruo, W., Tsuyoshi, I. (1998). The cell adhesion molecule E-cadherin is widely expressed in human atherosclerotic lesions, Cardiovascular. Res. 40: 191-205.
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)