Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Changes in Biochemical Parameters, Antioxidative Enzymes and Histopathology of Liver Induced by Cadmium (Cd) and Chlorpyrifos (CPF) in Wistar Rats

Y. Ravikumar, D. Madhuri, M. Lakshman, A. Gopala Reddy, B. Kalakumar
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1Department of Veterinary Pathology, College of Veterinary Science, Rajendranagar, Hyderabad- 500 030, Telangana, India.
Cite article:- Ravikumar Y., Madhuri D., Lakshman M., Reddy Gopala A., Kalakumar B. (2022). Changes in Biochemical Parameters, Antioxidative Enzymes and Histopathology of Liver Induced by Cadmium (Cd) and Chlorpyrifos (CPF) in Wistar Rats. Indian Journal of Animal Research. 56(9): 1090-1094. doi: 10.18805/IJAR.B-4178.
Background: Cd and CPF intoxication may occur directly through drinking water. Since the population tend to receive combination of multiple intoxicants through environment contamination, there is need for conducting studies to assess the impact of individual and combined environmental pollutants. The present research work was designed to study hepatotoxicity induced by Cd, CPF and their combination.
Methods: The experiment was carried out for 28 days in Wistar rats. G1: Control. G-2:CdCl2 @ 22.5mg/ kg b.wt / oral. G3: CPF @ 25 mg/ kg b.wt /per oral. G4:CdCl2@22.5 mg + CPF @ 25 mg/ kg b.wt /per oral. Biochemical parameters ware estimated from serum and liver samples were processed for tissue antioxidative parameters and histopathological examination. 
Result: Higher mean values of AST, ALT, ALP and lower liver GSH and SOD were observed in G2, 3 and 4 on 15th and 29th day when compared with G1. Liver in G2 and 3 showed mild degenerative changes, areas of necrosis and loss of architecture. In G4, lesions were moderate in severity. In addition, moderate perivascular fibrosis of portal triad was observed. The effects in combined group were severe than individual groups due to synergistic action of the combined pollutants.
  1. Aly, N., El-Gendy, K., Mahmoud, F. and El-Sebae, A.K. (2010). Protective effect of vitamin C against chlorpyrifos oxidative stress in male mice. Pesticide and Biochemical Physiology. 97: 7-12.
  2. Ambali, S., Joseph, O., King, A., Esievo, N. and Samuel, A. (2010). Hemotoxicity induced by chronic chlorpyrifos exposure in Wistar rats: Mitigating effect of vitamin C. Veterinary Medicine International. 1- 7.
  3. Asperlin A. (1994). Pesticide Industry Sales and Usage-1992 and 1993 Market Estimates. U.S. Environmental Agency Report No. 733-K-94-001. 
  4. Barski, D. and Spodniewska, A. (2018). Effect of chlorpyrifos and enrofloxacin on selected enzymes in rats. Polish Journal of Veterinary Sciences. 21(1): 39-46.
  5. Calderoni, A.M., Oliveros, L., Jahn, G., Anton, R., Luco, J. and Gimenez, M.S. (2005). Alterations in the lipid content of pituitary gland and serum prolactin and growth hormone in cadmium treated rats. Biometals. 18: 213-220. 
  6. Cheng, C.Y., Wong, E.W.P. and Lie, P.P.Y. (2011). Environmental toxicants and male reproductive function. Spermatogenesis. 1(1): 2-13. 
  7. Christian E.I., Olatoye T.R., Bamidele F.S., Ojo O.E. and Ademoye K.A. (2016). Cadmium-induced testicular toxicity, oxidative stress and histopathology in Wistar rats: Sustained effects of polyphenol-rich extract of Vernonia amygdalina (del.) leaf. Journal of Interdisciplinary Histopathology. 4(3): 54-62.
  8. Curcic. M., Sasa, J., Vesna, J., Sanja, V., Slavika, V., Ksenija, D., Zorica, B. and Biljana, A. (2012). Combined effects of cadmium and decabrominated diphenyl ether on thyroid hormones in rats. Arh Hig Rada Toksikol. 63: 255-262. 
  9. Dudley, R.E., Gammal, L.M. and Klaassen, C.D. (1985). Cadmium-induced hepatic and renal injury in chronically exposed rats: likely role of hepatic cadmium-metallothionein in nephrotoxicity. Toxicology and Applied Pharmacology. 77(3): 414-426.
  10. El-Sharaky, A.S., Newairy, A.A., Badreldeen, M.M., Ewada, S.M. and Sheweita, S.A. (2007). Protective role of selenium against renal toxicity induced by cadmium in rats. Toxicology. 235: 185-193.
  11. Gordon, C.J., Tholeatheus, A.G. and Ying, Y. (1997). Hypothermia and delayed fever in male rats exposed to chlorpyrifos. Toxicology. 118: 149-155. 
  12. Hassani, S., Sepand, M.R., Jafari, A., Jaafari, J., Rezaee, R., Zeinali, M., Tavakoli, F. and Razavi-Azarkhiavi, K. (2014). Protective effects of curcumin and vitamin E against chlorpyrifos-induced lung oxidative damage. Human Experimental Toxicolology. 1-9.
  13. Henson, M.C. and Chedrese, P.J. (2004). Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction. Experimental Biology and Medicine. 229(5): 383-392. 
  14. Jihen, E.H., Messaoudi, I., Fatima, H. and Kerkeni, A. (2008). Protective effects of selenium (Se) and zinc (Zn) on cadmium (Cd) toxicity in the liver and kidney of the rat: Histology and Cd accumulation. Food and Chemical Toxicology. 46(11): 3522-3527.
  15. Luna, G.L.H.T. (1968). Manual of Histological and Special Staining Techniques, 2nd Edition. 1-5 and 9-34, The Blakiston Divison McGraw-Hill Book Company, Inc. New York, Toronto, London.
  16. Madesh, M. and Balasubramanian, K.A. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Biophysics. 35(3): 184-188.
  17. Messaoudia, I., Banni, M., Lamia, S., Khaled, S. and Abdelhamid, K. (2010). Evaluation of involvement of testicular metallothionein gene expression in the protective effect of zinc against cadmium-induced testicular pathophysiology in rat. Reproductive Toxicology. 29: 339-345.
  18. Moron, M.S., Depierre, J.W. and Mannervik, B. (1979). Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects. 582(1): 67-78.
  19. Nasim Babaknejad, Ali Asghar Moshtaghie, Kahin Shahanipour and Somaye Bahrami (2015). The protective roles of zinc and magnesium in cadmium-induced renal toxicity in male Wistar rats. Iranian Journal of Toxicology. 8(27): 1160-1167.
  20. Pari, L. and Shagirtha, K. (2012). Hesperetin protects against oxidative stress related hepatic dysfunction by cadmium in rats. Experimental and Toxicologic Pathology. 64(5): 513-520.
  21. Poulsen, B.M. andersen, H.R. and Grandjean, P. (2008). Potential developmental neurotoxicity of pesticides used in Europe. Environmental Health. 7: 50. 
  22. Prabu, S. Milton, Muthumani, M. and K. Shagirtha., K. (2012). Protective effect of Piper betle leaf extract against cadmium- induced oxidative stress and hepatic dysfunction in rats. Saudi Journal of Biological Sciences. 19(2): 229-239.
  23. Rajendar, B., Bharavi, K., Rao, G.S., Kishore, P.V.S., Ravikumar, P., Satish Kumar, C.S.V. and Srinivaskumar, D. (2011). Protective Effect of á Tocopheral on biochemical and histological alterations induced by cadmium in rat testes. Indian J. Physiol Pharmacol. 55(3): 213-220.
  24. Ravikumar, Y., Madhuri, D., Lakshman, M., Gopala Reddy, A. and Kalakumar, B. (2019). Haematological alterations induced by cadmium (Cd) and chlorpyrifos (CPF) in male Wistar albino rats. Int. J. Curr. Microbiol. App. Sci. 8(8): 480-485. 
  25. Renugadevi, J. and Prabu, M. (2010). Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Experimental and Toxicologic Pathology. 62(2): 171-81.
  26. Sarkar, S., Yadav, P. and Bhatnagar, D. (1998). Lipid peroxidative damage on cadmium exposure and alterations in antioxidant system in rat erythrocytes: a study with relation to time. Biometals. 11: 153-157.
  27. Savithri, Y., Ravi Sekhar, P. and Jacob Doss, P. (2010). Biochemical and histopathological changes in liver due to chlorpyrifos toxicity in albino rats. Journal of the Indian society of Toxicology. 6(2): 5-10.
  28. Singh, R., Srivastava, A.K., Gangwar, N.K., Giri, D.K., Singh, R. and Kumar, R. (2016). Pathology of sub-chronic cadmium and Chlorpyrifos toxicity in broilers Indian Journal of Veterinary Pathology. 40(4): 331-336.
  29. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods, 8th edition, IOWAState University Press, Amer, IOWA, USA, 217-268.
  30. Tomaszewska, E., Winiarska-Mieczan, A. and Dobrowolski, P. (2015). Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea. Experimental and Toxicologic Pathology. 67(5): 331-339.
  31. Yongfeng Deng, Yan Zhang, Yifeng Lu, Yanping Zhao and Hongqiang Ren (2016). Hepatotoxicity and nephrotoxicity induced by the chlorpyrifos and chlorpyrifos-methyl metabolite, 3,5,6-trichloro-2-pyridinol, in orally exposed mice. Science of the Total Environment. 544: 507-514.

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