Clinico-pathological Diagnosis of Transmissible Venereal Tumour with Hepatozoon canis and Babesia canis Infection in a Chippiparai Dog

DOI: 10.18805/IJAR.B-4168    | Article Id: B-4168 | Page : 1121-1124
Citation :- Clinico-pathological Diagnosis of Transmissible Venereal Tumour with Hepatozoon canis and Babesia canis Infection in a Chippiparai Dog.Indian Journal of Animal Research.2021.(55):1121-1124
T. Mohanapriya, R. Ramprabhu, V. Kumar, P.A. Enbavelan, A. Ganesan priya.vet21@gmail.com
Address : Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli-627 358, Tamil Nadu, India. 
Submitted Date : 5-05-2020
Accepted Date : 30-09-2020


Background: The concurrent infection of tick borne haemoprotozoan diseases like B.canis, E.canis and H.canis were commonly recorded in breed dogs. There is paucity in recording the combined haemoprotozoan infection with neoplasms in the native breeds of Tamil Nadu. The current investigation was documented to study the clinico-pathological changes, diagnosis and treatment of the concomitant infection of Hepatozoon canis and Babesia canis along with transmissible venereal tumor in a native dog.
Methods: This Clinical investigation was carried out during April’ 2019 for about a month. A male Chippiparai dog was presented to the Veterinary Clinical Complex, VCRI, Tirunelveli with history of mass and bleeding from the genital area. The impression cytology from the mass and the whole blood and serum samples were collected for the laboratory diagnosis.
Result: The hematological examination showed marked anaemia and the blood smear revealed the presence of H.canis gamonts in neutrophils and B.canis piroplasm in red bloods cells. The serum biochemical values showed the elevation of alanine aminotransferase, alkaline phosphatase, blood urea nitrogen, creatinine and reduction of total protein, albumin. The impression cytology revealed the presence of uniform sheets discrete round cells with punctuate vacuolation in the cytoplasm, confirmed the sexually transmitted venereal tumor.


​B. canis Chippiparai dog Diagnosis H. canis Transmissible venereal tumor


  1. Balachandran, C., Sridhar, R., Pazhanivel, N., Anoopraj, R. (2010). A note on the incidence of babesia canis in a 10 day old pup on postmortem examination in Chennai, Tamil Nadu. Indian Journal Animal Research. 44(1): 73-75.
  2. Baneth, G., Samish, M., Alekseev, E., Aroch Shkap, I. (2001). Transmission of Hepatozoon canis to dogs by naturally-fed or percutaneously-injected Rhipicephalus sanguineus ticks. Journal of Parasitology. 87: 606-11.
  3. Brown, G.K., Canfield, P.J., Dunstan, R.H., Roberts, K., Martin, A.R., Brown, C.S., Irving, R. (2006). Detection of Anaplasma platys and Babesia canis vogeli and their impact on platelet numbers in free-roaming dogs associated with remote Aboriginal communities in Australia. Australian Veterinary Journal. 84: 321-5.
  4. Ganguly, B., Das, U., Das, A.K., (2013). Canine Transmissible venereal tumor: a review. Veterinary and Comparative oncology. 14: 1-12.
  5. Cingi, C.C., Yeni, D., Yazici, E., Cine, T.A., Ucar, M. (2020). Investigation of Transmissible Venereal Tumor in male dogs by cytological examination. Indian Journal of Animal Research. 54: 1-5.
  6. Harikrishnan, T.J., Pazhanivel, N., Chellappa, D.J. (2008). Observations on development of Hepatozoon canis in a dog. Journal of Veterinary Parasitology. 22: 35-40.
  7. Harrus, S., Aroch, I., Lavy, E., Bark, H. (1997). Clinical manifestations of infectious canine cyclic thrombocytopenia. Veterinary Record. 141: 247-50.
  8. Ibrahim, N.D., Rahamathulla, P.M., Njoku. (1989). Neutrophil myeloperoxidase deficiency associated with canine hepatozoonosis. International Journal for Parasitology. 19: 915-918.
  9. Irwin, P.J. (2009). Canine babesiosis: from molecular taxonomy to control. Parasites and Vectors. 2(1): S1-S4.
  10. Otter, W., Hack, M., John, J., Jacobs, L. Tan, J.F.V., Rozendaal, L., Van moorsela, R.J.A. (2015). Effective treatment of TVT in dogs with Vincristine and IL2. Anticancer Research. 35: 3385-3392.
  11. Paramjit, K., Deshmukh, S., Rajsukhbir, S., Bansal, B.K., Randhawa, C.S., Singla, L.D. (2012). Para-clinico-pathological observations of insidious incidence of canine hepatozoonosis from a mongrel dog: a case report. Journal of Parasitic Diseases. 36:135-138. 
  12. Sarma, K., Mondal, D.B., Saravanan, M., Kumar, M., Mahendran, K. (2012). Haemato-biochemical changes in Hepatozoon canis infected dog before and after therapeutic management. Journal of Veterinary Parasitology. 26: 35-38.
  13. Senthil. N.R., Subapriya, S., Vairamuthu, S. (2015). A report of Hepatozoon canis infection in a dog with transmissible venereal tumor. Macedonian Veterinary Review. 38 (2): 233-237. 
  14. Shiju simon, M., Chhavi Gupta, Sankar, P., Ramprabhu, R., Pazhanivel, N., Balachandran, C., Prathaban, S. (2016). Incidence of Transmissible Venereal Tumors in Dogs - A Survey of 278 Cases. Indian Veterinary Journal. 93: 72-73.
  15. Shyma, K.P., Veer Singh, Jay Prakash Gupta. (2019). In vitro evaluation of effectiveness of synthetic pyrethroids against brown dog tick. Indian Journal of Animal Research. 53(10): 1400-1402.
  16. Valenciano, A.C. and Cowell, R.L. (2014). Cowell and Tyler’s Diagnostic Cytology and Hematology of the Dog and Cat. 4th ed., Mosby, pp.70-71.
  17. Varshney, J.P., Deshmukh, V.V., Chaudhary, P.S. (2009). A Clinical Study of Tick-Borne Diseases in Puppies. Intas Polivet. 10: 387-391.

Global Footprints