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Evaluation of Double Ovsynch Protocol in Acyclic Jaffarabadi Heifers and Buffaloes with Respect to Ovarian Dynamics, Hormonal Profile and Fertility

DOI: 10.18805/ijar.B-4154    | Article Id: B-4154 | Page : 879-888
Citation :- Evaluation of Double Ovsynch Protocol in Acyclic Jaffarabadi Heifers and Buffaloes with Respect to Ovarian Dynamics, Hormonal Profile and Fertility.Indian Journal of Animal Research.2021.(55):879-888
R.J. Raval, K.B. Vala, R.J. Padodara, A.J. Dhami, F.S. Kavani rupeshraval@rediffmail.com
Address : College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.
Submitted Date : 22-04-2020
Accepted Date : 4-08-2020

Abstract

Background: Anestrus is one of the most commonly encountered infertility problems in cattle and buffalo in India. Jaffarabadi is one of the heaviest buffalo breeds of the world and is a native of Saurashtra region of Gujarat. The breed is known for poor reproductive efficiency. To improve its reproductive efficiency, this study was undertaken on acyclic Jaffarabadi animals employing double Ovsynch protocol and its assessment through ovarian dynamics and blood biochemical and endocrine profile on a farm. 
Methods: The study included acyclic post-pubertal Jaffarabadi heifers (age 42±2 and 48±2 months; Gr-I and Gr-II, n=6 each) and the postpartum lactating acyclic buffaloes (Gr-III, n=6) using a double Ovsynch protocol, which consisted of i/m injections of 20 µg Buserelin acetate on days 0, 10, 17 and 26 and 500 µg Cloprostenol sodium on days 7 and 24, with a timed insemination on day 27. Ovarian dynamics was monitored by performing ultrasonography using real-time B-mode ultrasound scanner together with blood sampling for hormonal and biochemical profile on each day of hormone therapy and then on days 0, 12, 21 and 35 post-AI. The animals inseminated at induced estrus/FTAI were followed for return to estrus, if any. Pregnancy was confirmed in non-return cases on day 35 by ultrasonography and on day 70 by per rectal examination. The findings on ovarian dynamics, plasma endocrine and biochemical profile were compared statistically between groups and periods and fertility rates between groups.
Result: In animals of treatment group I and II, a significant (p<0.05) increase in numbers of small follicles was observed on day 26. Number of large sized follicles was significantly (p<0.05) higher on day 26 in comparison to day 0 and day 7 in group III animals. Large and subordinate follicular diameters increased gradually, but the differences between periods were significant (p<0.001) only in group III with the highest diameter of large follicle on day 17. Plasma FSH and LH concentrations differed significantly (p<0.001) among groups at all-time intervals. Plasma LH in group I and III differed significantly (p<0.001). Plasma estrogen level was significantly (p<0.05) higher in group II than group III. Plasma progesterone concentrations in group I and II animals were significantly higher on day 35 post-AI. Plasma insulin levels were significantly (p<0.01) lower on all days for group III animals than group I and II. Blood glucose level was significantly (p<0.001) higher in group I on day 17 as compared to group II and III. Plasma total cholesterol was significantly (p<0.05) higher in group III as compared to group I and II. The conception rates at first service/FTAI in double Ovsynch treated animals of group I, II and III were 66.66, 83.33 and 16.66 %, respectively. Thus it was concluded that ovarian structures and the plasma endocrine profile reflected the ovarian response to different hormonal injections and that double Ovsynch protocol could be a better choice for improving conception rate in post-pubertal acyclic Jaffarabadi buffalo heifers as compared to multiparous acyclic buffaloes.

Keywords

Biochemical profile Conception rate Double Ovsynch Hormonal profile Jaffarabadi buffalo Ovarian dynamics

References

  1. Adams, G.P., Matteri, R.L., Kastelic, J.P., Ko, J.C.H. and Ginther, O.J. (1992). Association between surges of follicle-stimulating hormone and the emergence of follicular waves in heifers. Journal of Reproduction and Fertility. 94: 177-188.
  2. Adams, G.P., Nasser, L.F., Bo, G.A., Garcia, A., Del-Campo, M.R. and Mapletoft, R.J. (1994). Superovulatory response of ovarian follicles of wave 1 vs wave 2 in heifers. Theriogenology. 42: 1103-1113.
  3. Arthur, G.H., Noakes, D.E. and Pearson, H. (1989). Normal Reproductive Function. In: Veterinary Reproduction and Obstetrics. Bailliere Tindall, London, pp. 22-24.
  4. Astiz, S. and Fargas, O. (2013). Pregnancy per AI differences between primiparous and multiparous high-yield dairy cows after using Double Ovsynch or G6G synchronization protocols. Theriogenology. 79: 1065-1070.
  5. Barile, V.L., Terzano, G.M., Allegrini, S., Maschio, M., Razzano, M., Neglia, G. and Pacelli, C. (2007). Relationship among preovulatory follicle, corpus luteum and progesterone in oestrus synchronized buffaloes. Italian Journal of Animal Science. 6: 663-666.
  6. Baruselli, P.S., Muccicolo, R.G., Visinti, J.A., Viana, W.G., Arruda, R.B., Madureira, E.H., Olivera, C.A. and Molero-Filho, J.R. (1997). Ovarian follicular dynamics during estrous cycle in buffalo. Theriogenology. 47: 1531-1547.
  7. Batra, S.K. and Pandey, R.S. (1983). Luteinizing hormone in blood plasma of post-partum buffaloes (Bubalus bubalis). Theriogenology. 19(2): 193-200.
  8. Butler, W.R. and Smith, R.D. (1989). Interrelationship between energy balance and postpartum reproduction function in dairy cattle. Journal of Dairy Science. 72: 767-783.
  9. Chaudhary, N.J. , Patel, D.M., Dhami, A.J., Vala, K.B., Hadiya, K.K. and Patel, J.A. (2018). Effect of Doublesynch and Estra doublesynch protocols on estrus induction, conception rate, plasma progesterone, protein and cholesterol profile in anestrus Gir heifers. Veterinary World. 11(4): 542-548.
  10. Cirit, U, Ak, K. and Ileri, I.K. (2007). New strategies to improve the efficiency of the Ovsynch protocol in primiparous dairy cows. Bulletin of the Veterinary Institute in Pulawy. 51: 47-51.
  11. De Jarnette, J.M., Day, M.L., House, R.B., Wallace, R.A. and Marshall, C.E. (2001). Effect of GnRH pretreatment on reproductive performance of postpartum suckled beef cows following synchronization of estrus using GnRH and PGF2á. Journal of Animal Science. 79:1675-1682.
  12. Derar, R., Hussein, H. A., Fahmy, S., El-Sherry, T.M. and Megahed, G. (2012). The effect of parity on the efficacy of an ovulation synchronization (Ovsynch) protocol in buffalo (Bubalus bubalis). Animal Reproduction. 9: 52-60.
  13. Dirandeh, E., Roodbari, A.R., Colazo, M.G. (2015). Double-ovsynch, compared with presynch with or without GnRH, improves fertility in heat-stressed lactating dairy cows. Theriogenology. 83(3): 438-443.
  14. Duchens, M., Forsberg, M., Edquist, L.E., Gustafsson, H. and Rodriguez-Marhnes, H. (1994). Effects of suprabasal progesterone levels around estrus on plasma concentrations of progesterone, estradiol – 17â and LH in heifers. Theriogenology. 42: 1159-1169.
  15. Henricks, D.M., Dickey, J.F. and Hill, J.R. (1971). Plasma estrogen and progesterone level in cows prior to and during estrus. Endocrinology. 89: 1350.
  16. Herlihy, M.M., Giordano, J.O., Souza, A.H., Ayres, H., Ferreira, R.M., Keskin, A., Nascimento, A.B., Guenther, J.N., Gaska, J.M., Kacuba, S.J., Crowe, M.A., Butler, S.T. and Wiltbank, M.C. (2012). Presynchronization with Double-ovsynch improves fertility at first postpartum artificial insemination in lactating dairy cows. Journal of Dairy Science. 95(12): 7003-7014.
  17. Hoque, M.N., Kumar, A., Talukder, Akter, M. and Shamsuddin, M. (2014). Evaluation of ovsynch protocols for timed artificial insemination in water buffaloes in Bangladesh. Turkish Journal of Veterinary and Animal Sciences. 38: 418-424.
  18. Jayachandran, S., Nanjappan, K., Muralidharan, J., Selvaraj, P. and Manoharan, A. (2013). Blood biochemical and mineral status in cyclic and postpartum anestrus buffaloes. International Journal of Food, Agriculture and Veterinary Sciences. 3(1): 93-97.
  19. Jerome, A., Srivastava, S.K. and Sharma, R.K. (2016). Study on follicular characteristics, hormonal and biochemical profile in norgestomet + PMSG treated acyclic buffaloes. Iranian Journal of Veterinary Research. 17(4): 247-252.
  20. Kanai, Y. and Shimizu, H., (1984). Plasma concentration of luteinizing hormone, progesterone and oestradiol during the oestrous cycle in swamp buffaloes (Bubalus bubalis). Journal of Reproduction and Fertility. 70: 507-510.
  21. Kumar, A., Solanki, V.S., Jindal, S.K., Tripathi, V.N., Jain, G.C. (1997). Oocyte retrieval and histological studies of follicular population in buffalo ovaries. Animal Reproduction Science. 47: 189-195.
  22. Kumar, P.R., Shukla, S.N. and Purkayastha, R.D. (2013). Economic analysis of the estimated cost of management of anestrus buffaloes under field conditions using different hormonal and non-hormonal strategies. Journal of Animal Health and Production. 1(4): 39-41.
  23. Kumar, P.R., Singh, S.K., Kharche, S.D., Sharma, C.G., Behera, B.K., Shukla, S.N., Kumar, H., Agarwal, S.K. (2014). Anestrus in cattle and buffalo: Indian perspective. Journal Advanced Veterinary and Animal Science. 2(3): 124-138.
  24. Malik, R.K. (2005). Studies on ovarian follicular dynamics during early postpartum period, anestrus condition and hormonal therapies for induction of estrus in Murrah buffaloes (Bubalus bubalis). Ph.D. Thesis, CCS Haryana Agricultural University, Hisar (India).
  25. Martins, J.P., Policelli, R.K., Neuder, L.M., Raphael, W. and Pursley, J.R. (2011). Effects of cloprostenol sodium at final prostaglandin F2alpha of Ovsynch on complete luteolysis and pregnancy per artificial insemination in lactating dairy cows. Journal of dairy Science. 94: 2815-2824.
  26. Modi, L.C., Patel, P.A., Patel, S.P., Patel, G.G., Joshi, A.H. and Suthar, D.N. (2011). Prevalence of reproductive problems in buffalo in Mehsana milk–shed area of Gujarat. The International Journal of Agricultural Sciences and Veterinary Medicine. 5(4): 424-428.
  27. Mondal, S., Prakash, B.S. and Palta, P. (2004). Peripheral plasma FSH concentration in relation to expression of estrus in Sahiwal cattle (Bos indicus). Indian Journal of Physiology and Pharmacology. 48(2): 245-250.
  28. Moreira, F., Orlandi, C., Risco, C.A., Mattos, R., Lopes, F. and Thatcher, W.W. (2001). Effects of pre-synchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows. Journal of Dairy Science. 84: 1646-1659.
  29. Nebel, R.L. and McGilliard, M.L.(1993). Interactions of high milk yield and reproductive performance in dairy cows. Journal of Dairy Science. 76: 3257-3268.
  30. Parmar, K.H., Shah, R.G., Tank, P.H. and Dhami, A.J. (2012). Effect of hormonal and non-hormonal treatment on reproductive efficiency and plasma progesterone, biochemical and macro-minerals profile in postpartum anoestrus Surti buffaloes. Indian Journal of Field Veterinarians. 8(2): 48-54.
  31. Paul, V. and Prakash, B.S. (2005). Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed time artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology. 64: 1049-1060.
  32. Petersson, K.J., Gustafsson, H., Strandberg, E. and Berglund, B. (2006). Atypical progesterone profiles and fertility in Swedish dairy cows. Journal of Dairy Science. 89: 2529-2538.
  33. Pottapenjera, V., Rajanala, S.R., Reddy, C., Gangineni, A., Avula, K., Bejjanki, S.K., Sathagopam, S., Kesharwani, S. and Velmurugan S. (2018). Kisspeptin modulates luteinizing hormone release and ovarian follicular dynamics in pre-pubertal and adult Murrah buffaloes. Frontiers in Veterinary Science. 5: 149.
  34. Prajapati, J.P., Patel, D.M., Dhami, A.J., Patel, J.A., Hadiya, K.K. and Chaudhary, D.V. (2018). Role of various estrus induction and synchronization protocols in influencing plasma progesterone, metabolic profile and fertility in acyclic buffaloes. The Indian Journal of Veterinary Science and Biotechnology. 14: 1-7.
  35. Pursley, J.R., Mee, M.O. and Wiltbank, M.C. (1995). Synchronization of ovulation in dairy cows using PGF2 alpha and GnRH. Theriogenology. 44: 915-923.
  36. Roche, J.F. (2006). The effect of nutritional management of the dairy cow on reproductive efficiency. Animal Reproduction Science. 96: 282-296.
  37. Roy, K.S. and Prakash, B.S. (2008). Plasma luteinizing hormone profiles during ovsynch treatment in Murrah buffalo heifers. The Indian Veterinary Journal. 85: 678-679.
  38. Sarath, T., Singh, S.K., Arunmozhi, N., Rajkumar, R., Saxena, A. and Agrawal, S.K. (2016). Characterization of ovarian follicular development and steroid profile during estrous cycle and seasonal anestrous in buffalo. Indian Journal of Animal Science. 86(1): 28-31.
  39. Selvaraju, S., Bhat, K.S., Archana, S.S., Gowda, N.K.S., Krishnan, B.B., Redd, I.J., Pal, D.T., Roy, K.S. and Ravindra, J.P. (2017). Profile of plasma biomolecules and minerals in various reproductive status of cattle and buffaloes. Indian Journal of Animal Science. 87: 1071-1076.
  40. Senatore, E.M., Butler W.R. and Oltenacu P.A. (1996). Relationship between energy balance and post-partum ovarian activity and fertility in first lactation dairy cows. Animal Production. 62: 17-23.
  41. Sharma, K.B., Nayyar, S., Malik, V.S. and Sodhi, S.P.S. (1998). Biochemical studies in cyclic, anoestrous and suboestrus buffalo heifers. Indian Journal of Animal Science. 68(5): 469-470.
  42. Sheetal, S.K. (2017). Follicular development, superovulation and embryo recovery following insulin and insulin like growth factor-I (IGF-I) treatment during mid-luteal phase of estrous cycle in embryo donor and hormonal profile in recipient cattle. Ph.D. thesis, G.B. Pant University of Agriculture and Technology, Pantnagar, India.
  43. Shrivastava, O.P. and Kadu, M.S. (1995). Blood biochemical profiles in normal cycling and delayed pubertal crossbred heifers. Indian Journal of Animal Reproduction. 16(2): 91-92.
  44. Silva, E., Sterry, R.A. and Fricke, P.M. (2007). Assessment of a practical method for identifying anovular dairy cows synchronized for first postpartum timed artificial insemination. Journal of Dairy Science. 90: 3255-3262.
  45. Singh, B., Dixit, V.D., Singh, P., Georgie, G.C. and Dixit, V.P. (2001). Plasma inhibin levels in relation to steroids and gonadotrophins during the oestrus cycle in buffalo. Reproduction in Domestic Animals. 36(3-4): 163-167.
  46. Singh, B., Rawal, C.V.S and Singh, J.P. (2004). Studies on certain serum constituents in anoestrus and repeat breeder buffaloes. Indian Veterinary Medical Journal. 28: 261-263.
  47. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th Edn. Oxford and IBH Publishing Co., Calcutta, India.
  48. Solorzano, C.M.B., McCartney, C.R., Blank, S.K., Knudsen, K.L. and Marshall, J.C. (2010). Hyperandrogenemia in adolescent girls: origins of abnormal GnRH secretion. British Journal of Obstetrics and Gynaecology. 117(2): 143-149.
  49. Sousa, L.M.M.C., Silva, R.S., Bonfim Neto, A., Cardoso, A.P.M.M. and Papa, P.C. (2016). Insulin and CL function: lessons from studies in cattle and dogs. Animal Reproduction. 13(3): 373.
  50. Souza, A.H., Gumen, A., Silva, E.P., Cunha, A.P., Guenther, J.N., Peto, C.M., Caraviello, D. Z. and Wiltbank, M.C. (2007). Supplementation with estradiol-17â before the last gonadotropin-releasing hormone injection of the Ovsynch protocol in lactating dairy cows. Journal of Dairy Science. 90: 4623-4634.
  51. Souza, A.H., Ayres, H., Ferreira, R.M. and Wiltbank, M.C. (2008). A new presynchronization system (Double-ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology. 70(2): 208-215.
  52. Stevenson, J. (2017). Ovsynch goes double. Hoard’s dairy man. Available at http://hourds.com/print-article-4777-permanent. html> accessed 17 July, 2017
  53. Tanaka, T., Nagatani, S., Bucholtz, D.C, Ohkura, S., Tsukamura, H., Maeda, K. and Foster, D.L. (2000). Central action of insulin regulates pulsatile luteinizing hormone secretion in the diabetic sheep model. Biology of Reproduction. 62(5): 1256-1261.
  54. Twagiramungu, H., Guilbault, L.A., Proulx, J.G. and Dufour, J. (1994). Influence of corpus luteum and induced ovulation on ovarian follicular dynamics in postpartum cows treated with buserelin and cloprostenol. Journal of Animal Science. 42: 1796-1805.
  55. Varughese, E.E., Brar, P.S., Honparkhe, M. and Ghuman, S.P.S. (2014). Correlation of blood flow of the preovulatory follicle to its diameter and endocrine profile in dairy buffalo. Reproduction in Domestic Animals. 49: 140-144.
  56. Vasconcelos, J.L.M., Silcox, R.W., Rosa, G.J.M., Pursely, J.R. and Wiltbank, M.C. (1999). Synchronization rate, size of the ovulatory follicle and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology. 52: 1067-1078.
  57. Walsh, R.B., Kelton, D.F., Duffield, T.F., Leslie, K.E., Walton, J.S. and LeBlanc, S.J. (2007). Prevalence and risk factors for postpartum anovulatory condition in dairy cows. Journal of Dairy Science. 90: 315-324.
  58. Wiltbank, M.C., Arvalho, C.P.D., Keskin, A. (2011). Effect of progesterone concentration during follicle development on subsequent ovulation, fertilization and early embryo development in lactating dairy cows. Biology of Reproduction. 85: 685 (abstract).
  59. Wiltbank, M.C., Pursley, J.R., Fricke, P.M., Vasconcelos, J., Guenther, J.N. and Gibbons, J.R. (1996). Development of AI and ET Programs that do not require detection of estrus using recent information on follicular growth. Annual meeting of American Embryo Transfer Association. 15: 23-44.
  60. Yindee, M., Techakumphu, M., Lohachit, C., Sirivaidyapong, S., Na Chiangmai, A., Rodriguez Martinez, H. and Colenbrander, B. (2011). Follicular dynamics and estrous detection in Thai postpartum swamp buffaloes (Bubalus bubalis). Reproduction in Domestic Animals. 46(1): e91-e96.

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