Vitamins Supplementation Affecting Colostrum Composition in Murrah Buffaloes

DOI: 10.18805/ijar.B-4151    | Article Id: B-4151 | Page : 900-904
Citation :- Vitamins Supplementation Affecting Colostrum Composition in Murrah Buffaloes.Indian Journal of Animal Research.2021.(55):900-904
Vipin, Vishal Mudgal, Anurag Bharadwaj, A.K. Verma vishalmudgal1@rediffmail.com
Address : Division of Animal Nutrition and Feed Technology, ICAR-Central Institute for Research on Buffaloes, Hisar-125 001, Haryana India.
Submitted Date : 17-04-2020
Accepted Date : 18-06-2020


Background: Vitamins A and E are unable to get synthesized by animal’s body and due to unavailability of sufficient green fodder in the ration, supplementation is required. Buffaloes are the major milk producer dairy animal in India and hence a study was undertaken to evaluate the effect of supplementing vitamin A and E on the colostrum composition of peri-parturient Murrah buffaloes.  
Methods: Twenty-two multiparous Murrah buffaloes during their advance stage of pregnancy (-30 day) were selected and divided into three groups i. e. control (C, n=8), treatment 1 (T1, n=7) and treatment 2 (T2, n=7) based on the most probable producing ability of milk, parity and body weights. Buffaloes were fed either control diet (C) or supplemented with vitamin A and E @ 75,000 IU  and 1,500 IU/day, respectively, in group T1 and @ 1,50,000 IU and 3,000 IU/day, respectively in group T2. Colostrum samples were collected from one day after parturition up to day 5th after calving and analysed for fat, protein, lactose, solid-not-fat and total solids. 
Result: Supplementation of vitamin A and E at a higher level (T2) was helpful to increase (P<0.05) colostrum protein levels for first three days and total solids percentage for first two days as compared to control, while colostrum lactose and solid not fat % were high (P<0.05) in T1 groups as compared to control at day third only. The fat percentage had a reducing tendency (P<0.05) in control and T1 group and total solids in the T1 group only with the advancement of the post-parturient period. Based on the findings of the study, it may be concluded that vitamins A and E supplementation had a positive influence on the colostrum composition of peri-parturient Murrah buffaloes.


Buffaloes Colostrum Composition Vitamin A Vitamin E


  1. Abd El-Fattah, A.M., Abd Rabo, F.H.R., EL-Dieb, S.M. and El-    Kashef, H. A. (2012). Changes in composition of colostrum of Egyptian buffaloes and Holstein cows. BMC Veterinary Research 8: 19 (http://www.biomedcentral.com/1746-61 48/8/19).
  2. AOAC (2012). Official Methods of Analysis of the Association of Official Analytical Chemistry, 19th ed. AOAC International, Washington, USA.
  3. Basic Animal Husbandry Statistics (2019). Department of Animal Husbandry and Dairying, Krishi Bhawan, New Delhi. 
  4. Bell, J.A., Griinari, J.M. and Kennelly, J. J. (2006). Effect of safflower oil, flaxseed oil, monensin and vitamin E on concentration of conjugated linoleic acid in bovine milk fat. Journal of Dairy Science. 89: 733.
  5. Block, E. (2010). Transition cow research–What makes sense today. In Proceedings High Plains Dairy Conference (pp. 75-98).
  6. Capper, J.L., Wilkinson, R.G., Mackenzie, A.M. and Sinclair, L.A. (2006). Polyunsaturated fatty acid supplementation during pregnancy alters neonatal behavior in sheep. Journal of Nutrition. 136: 397-403.
  7. De Ondarza, M.B., Wilson, J.W. and Engstrom, M. (2009). Case study: Effect of supplemental â-carotene on yield of milk and milk components and on reproduction of dairy cows. The Professional Animal Scientist. 25(4): 510-516.
  8. Decaluwé, R., Maes, D., Wuyts, B., Cools, A., Piepers, S. and Janssens, G.P.J. (2014). Piglets’ colostrum intake associates with daily weight gain and survival until weaning. Livestock Science. 162: 185-192.
  9. Georgiev, I. and Penchev. (2005). Alterations in chemical composition of colostrum in relationship to post-partum time. Bulgarian Journal of Veterinary Medicine. 8: 35-39.
  10. Goff, J.P., Kimura, K. and Horst, R.L. (2002). Effect of mastectomy on milk fever, energy and vitamins A, E and â-carotene status at parturition. Journal of Dairy Science. 85: 1427-1436.
  11. Goff, J.P. and Stabel, J.R. (1990). Decreased plasma retinol, á-tocopherol and Zn concentration during the periparturient period: Effect of milk fever. Journal of Dairy Science. 73: 3195-3199.
  12. Hernández-Castellano, L.E., Morales-delaNuez, A., Sánchez-Macías, D., Moreno-Indias, I., Torres, A., Capote, J., Argüello, A. and Castro, N. (2015). The effect of colostrum source (goat vs. sheep) and timing of the first colostrum feeding (2h vs. 14h after birth) on body weight and immune status of artificially rare newborn lambs. Journal of Dairy Science. 98: 204-210.
  13. Hino, T., andoh, N. and Ohgi, H. (1993). Effects of â-carotene and á-tocopherol on rumen bacteria in the utilization of long chain fatty acids and cellulose. Journal of Dairy Science. 76: 600. 
  14. ICAR. (2013). Nutrient Requirements of Cattle and Buffalo. 3rd ed. Indian Council of Agricultural Research. New Delhi.
  15. Kehoe, S.I., Jayarao, B.M. and Heinrichs, A.J. (2007). A survey of bovine colostrum composition and colostrum management practices on Pennsylvania dairy farms. Journal of Dairy Science. 90: 4108-4116.
  16. Lotthammer, K.H. (1979). Importance of beta-carotene for the fertility of dairy cattle. Feedstuffs. 51: 16.
  17. Meza-Herrera, C.A. and Tena-Sempere, M. (2012). Interface between nutrition and reproduction: the very basis of production. In: [Astiz, S., Gonzalez-Bulnes, A. (Eds.)], An Imal Reproduction in Livestock. Encyclopedia of Life Support Systems (EOS), under the auspices of the UNESCO. EOS Publishers, Oxford, UK http://www.eolss.net.
  18. Morrill, K.M., Conrad. E., Lago, A., Campbell, J., Quigley, J. and Tyler, H. (2012). Nationwide evaluation of quality and composition of colostrum on dairy farms in the United States. Journal of Dairy Science. 95: 3997-4005.
  19. Mutoni, G., Prasad, S., De, K., Pal, S., Mukherjee, J., Kapila, S., Kapila, R., Kaur, h., Mohanty, A.K. and Dan, A.K. (2012). Effect of supplementation of vitamin E, copper and zinc around peripartum on udder health, milk yield and composition of Sahiwal cows. Livestock Research for Rural Development, 24 (12) Article #220. Retrieved April 1, 2020 from http://www.lrrd.org/lrrd24/12/muto2422.h tm.
  20. Nørgaard, J.V., Nielsen, M.O., Theil, P.K., Sørensen, M.T., Safayi, S. and Sejrsen, K. (2008). Development of mammary glands of fat sheep submitted to restricted feeding during late pregnancy. Small Ruminant Research. 76: 155-165.
  21. O’Doherty, J.V. and Crosby, T.F. (1996). The effect of diet in late pregnancy on progesterone concentration and colostrum yield in ewes. Theriogenology. 46: 233-241.
  22. Oldham, E.R., Eberhart, R.J. and Muller, L.D. (1991). Effects of supplemental vitamin A or â-carotene during the dry period and early lactation on udder health. Journal of Dairy Science. 74(11): 3775-3781.
  23. Oliveira, R.C., Guerreiro, B.M., Junior, N.M., Araujo, R.L., Pereira, R.A.N. and Pereira, M.N. (2015). Supplementation of prepartum dairy cows with â-carotene. Journal of Dairy Science 98(9): 6304-6314.
  24. Panda, N., Kaur, H. and Mohanty, T.K. (2006). Reproductive performance of dairy buffaloes supplemented with varying levels of vitamin E. Asian-Australasian Journal of Animal Science. 19(1): 19.
  25. Patto, R.A., Singh, D.V., Singh, S.K., Singh, M.K., Singh, A.K. and Kaushal, S. (2016). Colostrum and milk composition during postpartum period in Hill cow, sahiwal and cross breds cow. Indian Journal Of Animal Research. 50: 211-214.
  26. Pottier, J., Focant, M., Debier, C., De Buysser, G., Goffe, C., Mignolet, E., Froidmont, E. and Larondelle. Y. (2006). Effect of dietary vitamin E on rumen biohydrogenation pathways and milk fat depression in dairy cows fed high-fat diets. Journal of Dairy Science. 89: 685.
  27. Nieto, R.C.A., Meza-Herrera, C.A., Morón Cedillo, F.J., Flores Najera, M.J., Gámez Vázquez, H.G., Cuevas Reyes, V. and Liu, S.M. (2015). Effects of vitamin E supply during late gestation and early lactation upon colostrum composition, milk production and quality in nutritional restricted ewes. Small Ruminant Research. 133: 77-81.
  28. SPSS. (2010). Statistical packages for Social Sciences, Version 16, SPSS Inc., Illinois, USA.
  29. Van Soest, P.J., RobertsAon, G.B. and Lewis. B.A. (1991). Symposium: Carbohydrate methodology, metabolism and nutritional implications in dairy cattle Journal of Dairy Science. 74: 3583-3597.
  30. Weiss, W.P., Hogan, J.S. and Smith, K.L. (1994). Use of á-tocopherol concentrations in blood components to assess vitamin E status to dairy cows. Agricultural Practice. 15: 5-8.

Global Footprints