Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 7 (july 2021) : 763-766

Superovulatory Response and Progesterone Profile in Murrah Buffaloes (Bubalus bubalis) Pretreated with GnRH Agonist

Sumit Singhal, Shiv Prasad, Rajesh Verma, H.P. Gupta, J.K. Prasad
1Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, Uttarakhand, India.
Cite article:- Singhal Sumit, Prasad Shiv, Verma Rajesh, Gupta H.P., Prasad J.K. (2020). Superovulatory Response and Progesterone Profile in Murrah Buffaloes (Bubalus bubalis) Pretreated with GnRH Agonist. Indian Journal of Animal Research. 55(7): 763-766. doi: 10.18805/IJAR.B-4133.
Background: Limited elite buffalo population need to be propagated at faster rate to sustain the dairy industry in India. Faster propagation could be achieved through embryo transfer technology. Low serum progesterone concentrations during the start of superstimulatory treatment is major factor that hampers the success of multiple ovulation embryo transfer (MOET) in buffaloes. This study evaluated the effect of GnRH pretreatment before superovulatory regimen on progesterone profile, superovulation and correlation of progesterone with superstimulatory parameter in Murrah buffaloes. 
Methods: Buffaloes (n=27) were superstimulated using 600 mg Folltropin and divided into three groups on basis of pretreatment: Group I and group II were pretreated with GnRH @ 10 µg and 06 µg, respectively while no pretreatment was subjected in group III (control). Progesterone hormone concentration, superovulatory response, ovulation rate, embryo recovery were recorded. 
Result: Our study revealed that superovulatory response was non-significantly different in all three groups and ranged from 6-8 in terms of number of corpus luteum (CL). Average number of recovered embryos in this study was nearly 2.0. A positive correlation between progesterone concentrations at the initiation of FSH treatment with number of CL and embryo recovery was observed. The study indicated that GnRH pretreatment before superstimulation improve the serum progesterone concentration, superovulatory response and embryo recovery in Murrah buffaloes. 
  1. Baruselli, P.S., Ferreira, R.M., Sales, J.N.S., Gimenes, L.U., Sá Filho, M.F., Martins, C.M., Rodrigues, C.A., Bó, G.A. (2011). Timed embryo transfer programs for management of donor and recipient cattle. Theriogenology. 76: 1583-1593.
  2. Baruselli, P.S., Madureira, E.H., Visintin, J.A., Porto Filho, R.M., Carvalho, N.A.T., Campanile, G., Zicarelli, L. (2000). Failure oocyte entry into oviduct in superovulated buffalo. Theriogenology. 53: 491 (Abstract).
  3. Beg, M.A., Sanwal, P.C., Yadav, M.C. (1997). Ovarian response and endocrine changes in buffalo superovulated at midluteal and late luteal stage of the estrous cycle: a preliminary report. Theriogenology. 47: 423-32.
  4. Colli, L., Milanesi, M., Vajana, E., Iamartino, D., Bomba, L., Puglisi, F., … Ajmone-Marsan, P. (2018). New insights on water buffalo genomic diversity and post-domestication migration routes from medium density SNP chip data. Frontiers in Genetics. 9: 1-17. 
  5. DADF. (2019). Release Page. aspx? PRID= 1588304.
  6. Dutta, T.K., Taneja, V.K. and Umashankar. (1992). Superovulation response of Haryana cows with PMSG/FSH. Indian J. of Anim. Sci. 62: 432-33.
  7. FAOSTAT.(2010). Default. aspx?PageID0573#ancor
  8. Halbert, G.W., Leslie, K.E., Walton, J.S. and Betteridge, K.J. (1989). Evaluation of return to estrus in superovulated dairy heifers following prostaglandin treatment. Theriogenology. 31: 201 (Abstract).
  9. Heleil, B. and El-Deeb Y. (2010). Superovulatory response in relation to follicular dynamics and presence of dominant follicles in Egyptian buffaloes. Adv. Biol. Res. 4(3): 169-174. 
  10. Ireland, J.J. and Roche, J.F. (1982). Development of antral follicles in cattle after prostaglandin induced luteolysis: Changes in serum hormones, steroids in follicular fluid and gonadotropin receptors. Endocrinology. 111: 2077-2086.
  11. Jindal, R., Gill, S.P.S., Setia, M.S. and Rattan, P.J.S. (1988). Estrus synchronization in buffaloes using lutalyse. Buffalo Bulletin. 7: 61-67.
  12. Kharche, S.D., Sharma, G.T., Agrawal, S.K., Majumdar, A.C. and Sharma N.C. Sanwal, P.C. (2002). Endocrine profile and embryo quality of cows superovulated with super-Ov following effect of human chorionic gonadotropin and estradiol -17β pretreatment. Indian J. Anim. Sci. 72(8): 635-639.
  13. Madan, M.L., Singla, S.K., Singh, C., Prakash, B.S. and Jailkhani, S. (1998). Embryo transfer technology in buffaloes: Endocrine responses and limitations. Proceeding of II World Buffalo Congress, New Delhi. 3: 195-211.
  14. Misra, A.K. and Tyagi, S. (2007). In vivo embryo production in buffalo: present and perspectives. Ital. J. Anim. Sci. 6: 74-91.
  15. Misra, A.K., Jaiswal, R.S., Kasiraj, R., Mutha Rao, M., Rangareddy, N.S., Pant, H.C. (2000). Periovulatory steroid hormone profiles during unstimulated and superovulatory oestrus cycle in buffalo (Bubalus bubalis). Buffalo Journal. 3: 371-80.
  16. Mohammed, K.M.E. (2018). Application of advanced reproductive biotechnologies for buffalo improvement with focusing on Egyptian buffaloes. Asian Pac. J. Reprod. 7(5): 193-205.
  17. Neglia, G., Gasparrini, B., Vecchio, D., Di Palo, R., Zicarelli, L., Campanile, G. (2010). Progesterone supplementation during multiple ovulation treatment in buffalo species (Bubalus bubalis). Tropical Animal Health and Production. 42: 1243-1247.
  18. Phogat, J.B., Pandey, A.K. and Singh, I. (2016). Seasonality in buffaloes reproduction. International Journal of Plant, Animal and Environmental Sciences. 6: 46-55.
  19. Ravi, S.K., Prasad, S., Singh, B., Prasad, J.K. and Singhal, S. (2011). Hormonal profile in superovulated buffaloes following ablation of dominant follicle. The Indian Veterinary Journal. 93(07): 19-21.
  20. Singh, N., Dhaliwal, G.S., Malik, V.S., Dadarwal, D., Honparkhe, M., Singhal, S., et al. (2015). Comparison of follicular dynamics, superovulatory response and embryo recovery between estradiol based and conventional superstimulation protocol in buffaloes (Bubalus bubalis). Veterinary World. 8(8): 983-988.
  21. Singhal, S., Singh, N., Singh, H., Malik, V.S. and Brar, P.S. (2021). Curtailing the conventional dose of Folltropin-V for superstimulation and embryo recovery in Sahiwal cattle (Bos Indicus): a novel concept. Ind. J. Anim. Sci. (accepted)
  22. Situmorang, P. (2008). A controlled intra-vaginal device releasing hormone for superovulation program in buffalo. Animal Production. 10(1): 5-11.
  23. Taneja, M., Singh, G., Totey, S.M. and Ali, A. (1995). Follicular dynamics in water buffalo superovulated in presence or absence of a dominant follicle. Theriogenology. 44: 581-97.

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