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Effect of LH and Estradiol-17β Supplementation at Different Time Points on In vitro Development of Preantral Follicles in Sheep

DOI: 10.18805/IJAR.B-4119    | Article Id: B-4119 | Page : 751-757
Citation :- Effect of LH and Estradiol-17β Supplementation at Different Time Points on In vitro Development of Preantral Follicles in Sheep.Indian Journal of Animal Research.2021.(55):751-757
L.S.S. Varaprasad Reddy, B.R. Naik, A.V.N. Sivakumar, B. Punyakumari, J. Suresh shivavet@gmail.com
Address : Department of Veterinary Physiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517 502, Andhra Pradesh, India.
Submitted Date : 11-03-2020
Accepted Date : 1-09-2020

Abstract

Background: Ovarian follicular development and growth are controlled by many hormones and growth factors. Despite the fact that LH and estradiol-17β have been utilized for the in vitro culture of preantral follicles yet, the suitable time points of supplementation of LH and estradiol-17β is not known. Therefore this study aimed to investigate the influence of addition of LH and estradiol-17β at different time points on in vitro development of preantral follicles (PFs’) in sheep. 
Method: Preantral follicles isolated from the ovarian cortical slices using micro dissection method were cultured for six days in Bicarbonate buffered Tissue culture medium 199B (TCM 199B) or in a standard culture medium supplemented with LH (2 μg/ml) and estradiol-17β (5 ng/ml) at different points during the culture period. COCs isolated from the follicles at the end of six day culture in different treatments were subjected to in vitro maturation for additional 24h. 
Result: Supplementation of LH and estradiol-17β during last two days of the culture supported better proportion of PFs’ exhibiting growth whereas supplementation of LH and estradiol-17β during first two days of the culture supported better average increase in diameter and proportion of PFs’ exhibiting antrum formation at the end of six day culture. Further the oocytes in COCs isolated at the end of culture in these treatments and subsequently subjected to in vitro maturation (IVM) for 24hr developed at a higher frequency to MII (metaphase II) stage. Supplementation of LH and estradiol-17β to TCM 199B culture medium in early stages followed by standard medium alone in later stages supports better development of PFs’ in vitro. Following supplementation with LH and estradiol-17β for the first two days culture of PFs’ in standard medium appears to be advantageous for the development of preantral follicles in vitro.

Keywords

Estradiol-17β In vitro LH Preantral follicles Sheep

References

  1. Abir, R., Franks, S., Mobberley, M.A., Moore, P.A., Margara, R.A. and Winston, R.M. (1997). Mechanical isolation and In vitro growth of preantral and small antral human follicles. Fertility and Sterility. 68: 682-688.
  2. Andrade, E.R., Maddox-Hyttel, P., Landim-Alvarenga, F.D.C., Viana Silva, J.R., Alfieri, A. A., Seneda, M.M., Figueiredo, J.R. and Toniolli, R. (2011). Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF and FSH. Veterinary Medicine International. 2011: 1-7. doi:10.4061/2011/670987.
  3. Araújo, V.R., Gastal, M.O., Figueiredo, J.R and Gastal, E.L. (2014). In vitro culture of bovine preantral follicles: a review. Reproductive Biology and Endocrinology. 12: 78.
  4. Arunakumari, G., Shanmugasundaram, N. and Rao, V.H. (2010). Development of morulae from the oocytes of cultured sheep preantral follicles. Theriogenology. 74: 884-894.
  5. Arunakumari, G., Amin. R.U., Sadasiva Rao, K., Teja, A. and Ramesh, T. (2013). Effects of isolating methods and different concentrations of hormones and growth factors on in vitro development of preantral follicles from goat fetuses. International Journal of Recent Scientific Research. 4: 259-265.
  6. Cadenas, J., Leiva-Revilla, J., Vieira, L.A., Apolloni, L.B., Aguiar, F.L.N., Alves, B.G., Lobo, C.H., Rodrigues, A.P.R., Apgar, G.A., Smitz, J., Figueiredo, J.R. and Maside, C. (2017). Caprine ovarian follicle requirements differ between prenatal and early antral stages after IVC in medium supplemented with GH and VEGF alone or in combination. Theriogenology. 87: 321-332.
  7. Cortvrindt, R., Hu, Y. and Smith, J. (1998a). Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin. Fertility and Sterility. 70: 1114-1125.
  8. Cortvrindt, R., Hu, Y. and Smith, J. (1998b). Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone supplemented mouse preantral follicle culture. Human Reproduction. 13: 1292-1302.
  9. Costa, S.L., Costa, E.P., Pereira, E.C.M., Gonçalves, W.G., Silva, T.F. and Queiroz, V.L.D. (2014). Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles. The Revista Brasileira de Ciencia do Solo. 21: 110-116.
  10. Craig, Z.R., Singh, J., Gupta, R.K. and Flaws, J.A. (2014). Co-treatment of mouse antral follicles with 17â-estradiol interferes with mono-2-ethyhexyl phthalate (MEHP)-induced atresia and altered apoptosis gene expression. Reproductive Toxicology. 45: 45-51.
  11. de Figueiredo, J.R, Celestino, J.J.H, Faustino, L.R and Rodrigues, A.P.R. (2011). In vitro culture of caprine preantral follicles: advances, limitations and prospects. Small Rumin Res. 98: 192-195.
  12. Endo, M., Kawahara-Miki, R., Cao, F., Kimura, K., Kuwayama, T., Monji, Y. and Iwata, H. (2013). Estradiol supports in vitro development of bovine early antral follicles. Reproduction. 145: 85-96.
  13. Flaws, J.A., Abbud, R., Mann, R.J., Nilson, J.H. and Hirshfield, A.N., (1997). Chronically elevated luteinizing hormone depletes primordial follicles in the mouse ovary. Biology of Reproduction. 57: 1233-1237.
  14. Garverick, H.A., Baxter, G., Gong, J., Armstrong, D.G., Campbell, B.K., Gutierrez, C.G. and Webb, R. (2002). Regulation of expression of ovarian mRNA encoding steroidogenic enzymes and gonadotropin receptors by FSH and GH in hypogonadotrophic cattle. Reproduction. 123: 651-661. 
  15. Lima, L.F., Rocha, R.M.P., Duarte, A.B.G., Brito, I.R., Silva, G.M., Rodrigues, G.Q., Nunes-Pinheiro, D.C.S., Sales, A.D., Moura, A.A., Wheeler, M.B., Rodrigues, A.P.R., Campello, C.C. and Figueiredo, J.R. (2016). Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles. Microscopy Research and Technique. 80(4): 406-418.doi:10.1002/jemt.22810.
  16. Lima-Verde, I.B., Saraiva, M.V., Matos, M.H., Bruno, J.B., Teno´rio, S.B., Martins, F.S., Rossetto, R., Cunha, L.D, Name, K.P., Báo, S.N, Campello, C.C. and Figueiredo, J.R. (2010). Interaction between estradiol and FSH promotes in vitro survival and development of caprine preantral follicles. Cells Tissues Organs. 191: 240-247.
  17. Liu, J., Rybouchkin, A., Van der Elst, J. and Dhont, M. (2002). Fertilization of mouse oocytes from in vitro matured preantral follicles using classical in vitro fertilization or intracytoplasmic sperm injection. Biology of Reproduction. 67: 575-579.
  18. Markstrom, E., Svensson, E., Shao, R., Svanberg, B and Billing, H. (2002). Survival factors regulating ovarian apoptosis-dependence on follicle differentiation. Reproduction. 123: 23-30.
  19. Martins, F.S., Celestino, J.J., Saraiva, M.V., Chaves, R.N., Rossetto, R., Silva, C.M.G., Lima-Verde, I.B., Lopes, C.A.P., Campello, C.C. and Figueiredo, J.R. (2010). Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles. Brazilian Journal of Medical and Biological Research. 43: 728-736.
  20. Monte, A.P.O., Barros, V.R.P., Santos, J.M., Menezes, V.G., Cavalcante, A.Y.P., Gouveia, B.B., Bezerra, M.E.S., Macedo, T.J.S. and Matos, M.H.T. (2019). Immuno histo chemical localization of insulin-like growth factor-1 (IGF-1) in the sheep ovary and the synergistic effect of IGF-1 and FSH on follicular development in vitro and LH receptor immunostaining. Theriogenology. 129: 61-69. doi:10.1016 /j.theriogenology.2019.02.005
  21. Murdoch, W.J. (1998). Inhibition by oestrdiol of oxidative stress induced apoptosis in pig ovarian tissue. Journal of Reproduction and Fertility. 114: 127-130.
  22. Nelson, L.R and Bulun, S.E. (2001). Estrogen production and action. Journal of American Acadamy Dermatology. 45: 116-124.
  23. Orisaka, M., Hattori, K., Fukuda, S., Mizutani, T., Miyamoto, K., Sato, T., Tsang, B. K., Kotsuji, F. and Yoshida, Y. (2013). Dysregulation of ovarian follicular development in female rat: LH decreases FSH sensitivity during preantral-early antral transition. Endocrinology. 154: 2870-2880.
  24. Park, Y.H., Gong, S.P., Kim, H.Y., Kim, G.A., Choi, J.H., Ahn, J.Y. and Lim, J.M. (2013a). Development of a serum-free defined system employing growth factors for preantral follicle culture. Molecular Reproduction and Development. 80: 725-733.
  25. Park, K.E., Ku, S., Jung, K.C., Liu, H.C., Kim, Y.Y., Kim, Y.J., Kim, S.H., Choi, Y.M., Kim, J.G. and Moon, S.Y. (2013b). Effect of urinary and recombinant gonadotropins on in vitro maturation of mouse preantral follicles. Reproductive Sciences. 20: 909-916.
  26. Picton, H.M., Harris, S.E., Muruvi, W and Chambers, E.L. (2008). The in vitro growth and maturation of follicles. Reproduction. 136: 703-715.
  27. Richards, J.S., Jahnsen, T., Hedlin, L., Lifka, J., Ratoosh, S., Durica, J.M. and Goldring, N.B. (1986). Ovarian follicle development: from physiology to molecular biology. Recent Progress in Hormone Research. 43: 231-276.
  28. Richards, J., Fitzpatrick, S., Clemens, J., Morris, J., Allistone, T and Sirosis, J. (1995). Ovarian cell differentiation: a cascade of multiple hormones, cellular signals and regulated genes. Recent Prog Horm Res. 50: 223-254. 
  29. Saraiva, M.V.A., Celestino, J.J.H., Chaves, R.N., Martins, F.S., Bruno, J.B., Lima-Verde, I.B., Matos, M.H.T., Silva, G.M., Porfirio, E.P., Báo, S.N., Campello, C.C., Silva, J.R.V., Figueiredo, J.R., (2008). Influence of different concentrations of LH and FSH on in vitro caprine primordial ovarian follicle development. Small Ruminant Research. 78: 87-95.
  30. Silva, G.M., Rossetto, R., Chaves, R.N., Duarte, A.B.G., Araújo, V.R., Feltrin, C., Bernuci, M.P., Anselmo-Franci, J.A., Xu, M., Woodruff, T.K., Campello, C.C. and Figueiredo, J.R. (2015). In vitro development of secondary follicles from pre-pubertal and adult goats cultured in two-dimensional or three-dimensional systems. Zygote. 23: 475-484.
  31. Tasaki, H., Iwata, H., Sato, D., Monji, Y. and Kuwayama, T. (2013). Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro. Theriogenology. 79: 809-814.
  32. Tomic, D., Frech, M.S., Babus, J.K., Symonds, D., Furth, P.A., Koos, R.D and Flaws, J.A. (2007). Effect of ERá overexpression on female reproduction in mice. Reproduction Toxicology. 23: 317-325.
  33. Wu, J., Nayudu, P.L., Kiesel, P.S. and Michelmann, H.W. (2000). Luteinizing hormone has a stage-limited effect on preantral follicle development in vitro. Biology of Reproduction. 63: 320-327.
  34. Zheng, P., Si, W., Bavister, B.D., Yang, J., Ding, C. and Ji, W. (2003). 17â-estradiol and progesterone improve in vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys. Human Reproduction. 18: 2137-2144.

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