Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 7 (july 2021) : 853-859

Optimization of Perilla, Roselle and Zanthoxylum Levels in Pork Patties by Application of Conjoint Analysis

Rongsensusang, V. Appa Rao, R. Narendra Babu, R. Karunakaran, R. Palani Dorai, A. Serma Saravana Pandian
1Department of Livestock Products Technology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai- 600 007, Tamil Nadu, India.
Cite article:- Rongsensusang, Rao Appa V., Babu Narendra R., Karunakaran R., Dorai Palani R., Pandian Saravana Serma A. (2020). Optimization of Perilla, Roselle and Zanthoxylum Levels in Pork Patties by Application of Conjoint Analysis. Indian Journal of Animal Research. 55(7): 853-859. doi: 10.18805/ijar.B-4118.
Background: Perilla, roselle and zanthoxylum are spices which are commonly used in preparation of meat dishes. They provide unique and distinctive flavour and taste which improves the sensory properties of the products to which they are added. In addition, they also possess desirable functional properties like antioxidant and antimicrobial properties of natural origin. The objective of the study was to optimize the inclusion levels in combination with the most acceptable sensory quality and to study the storage stability of pork patties at refrigerated (4±1oC) temperature. 
Methods: Three different levels of perilla 0.5, 0.7 and 0.9%, roselle 0.2, 0.3 and 0.4% and zanthoxylum 0.1, 0.15 and 0.2% were used in nine (9) different combinations designated as C1, C2, C3, C4, C5, C6, C7, C8, and C9 in the preparation of pork patties, to determine the most preferred combination according to physicochemical and sensory analysis. The patties were analyzed for pH, emulsion stability, colour and sensory evaluation. Sensory quality of patties was conducted and rating score for each profile, contribution of each attribute to the respondent’s preference was calculated by using conjoint analysis procedure. 
Result: The results of conjoint analysis revealed that the combination with perilla 0.5%, roselle 0.2% and zanthoxylum 0.1% was the most preferred combination as compared to all other combinations. Treated patties show lower oxidative and microbial parameters values and higher sensory scores over a period of 21 days.
  1. APHA (1984). Compendium of Methods for Microbiological Examination of Foods, 2nd edn. American Public Health Association, Washington.
  2. Baliga, B.R and Madaiah, N. (1970). Quality of sausage emulsion prepared from mutton. Journal of Food Science. 35: 383-385.
  3. Blois, M.S. (1958). Antioxidant determination by the use of a stable free radical. Nature. 181: 1199-1200.
  4. Borch, E., Kant-Muermans, M.L., Blixt, Y. (1996). Bacterial spoilage of meat and cured meat products. International Journal of Food Microbiology. 33: 103-120.
  5. Bourne M.C, J.F. Kenny J F, Barnard J, (1978). Computer-assisted readout of data from texture profile analysis curves. Journal of Texture Studie. 9: 481-494.
  6. Bozkurt, H and Belibagli, K. B. (2009). Use of rosemary and Hibiscus sabdariffa in production of kavurma, a cooked meat product. Journal of Science Food Agriculture. 89: 1168-1173.
  7. Cardeal, Z.D.L., Gomes da Silva, M.D.R. and Marriott, P.J. (2006). Comprehensive two-dimensional gas chromatography/mass spectrometric analysis of pepper volatiles. Rapid Communications in Mass Spectrometry: An International Journal Devoted to the Rapid Dissemination of Up-to-the-Minute Research in Mass Spectrometry, 20(19): 2823-2836.
  8. Chao, C.Y and Yin, M.C. (2009). Antibacterial Effects of Roselle Calyx Extracts and Protocatechuic Acid in Ground Beef and Apple Juice. Foodborne Pathogens and Disease. 6: 201-206.
  9. Chumsri, P, A. Sirichote and A. Itharat (2008) Studies on the optimum conditions for the extraction and concentration of roselle (Hibiscus sabdariffa Linn.) extract, Songklanakarin J. Sci. Technol. 30 (Suppl.1): 133-139.
  10. Hair, J. F., R.E. Anderson, R. L. Tatham and W.C. Black, (1998). Multivariate Data Analysis. 5th Ed. Upper Saddle River, New Jersey. Prentice-Hall International. 
  11. Ji, Y., Li, S. and Ho, C.T. (2019). Chemical composition, sensory properties and application of Sichuan pepper (Zanthoxylum genus). Food Science and Human Wellness. 8(2): 115-125.
  12. Joshi P, Raghuvanshi, R.S., Sankhala, A., Sharma, V. (2016). Proximate composition and antioxidant potential of fruits of zanthoxylum alatum roxb. (tooth ache tree) and its acceptability assessment in food products. Current Research in Nutrition and Food Science. 4(1): 74-79.
  13. Jung, S., Choe, J.H., Kim, B., Yun, H., Kruk, Z.A. and Jo, C. (2010). Effect of dietary mixture of gallic acid and linoleic acid on antioxidative potential and quality of breast meat from broilers. Meat Science. 86(2): 520-526.
  14. Jung, E and Joo, N., (2013). Roselle (Hibiscus sabdariffa L.) and soybean oil effects on quality characteristics of pork patties studied by response surface methodology. Meat Sci. 94: 391-401.
  15. Kanwal, R., Arshad, M., Bibi, Y., Asif, S. and Chaudhari, S.K. (2015). Evaluation of Ethnopharmacological and Antioxidant Potential of Zanthoxylum armatum DC. Journal of Chemistry, 2015: 1-8.
  16. Keeton J.T. (1983). Effect of fat and sodium chloride phosphate levels on the chemical and sensory properties of pork patties. J Food Sci. 36: 261-276.
  17. Kim, S.J., H.N. Yoon and Rhee, J.S. (2000). The effects of roasting temperatures on the formation of headspace volatile compounds in perilla seed oil. Journal of the American Oil Chemists' Society. 77(4): 451.
  18. Koniecko, R., (1979). Handbook for Meat Chemists. Avery Publishing Group, Inc. Wayne, New Jersy, 53-55.
  19. Kumar, P., M.K. Chatli, N. Mehta, O.P. Malav, A.K. Verma, D. Kumar and M. Rathour, (2018). Antioxidant and Antimicrobial Efficacy of Sapota Powder in Pork Patties Stored under Different Packaging Conditions. Korean J Food Sci Anim Resour. 38(3): 593-605.
  20. Longo, M.A. and Sanromán, M.A. (2006). Production of food aroma compounds: microbial and enzymatic methodologies. Food Technology and Biotechnology. 44(3): 335-353.
  21. Mohamad, O., B. Mohd-Nazir, M. Abdul Rahman and S. Herman, (2002). Roselle: A new crop in Malaysia. Buletin PGM. 8: 12-13.
  22. Ran, M., Chen, C., Li, C., He, L., Zeng, X., (2020). Effects of replacing fat with Perilla seed on the characteristics of meatballs, Meat Scienc. 161: 107995.
  23. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26(9-10): 1231-1237.
  24. Sargi, S.C., Silva, B.C., Santos, H.M.C., Montanher, P.F., Boeing, J.S., Santos Júnior, O.O., Souza, N.E. and Visentainer, J.V., (2013). Antioxidant capacity and chemical composition in seeds rich in omega-3: chia, flax and perilla. Food Science and Technology. 33(3): 541-548.
  25. Seitz, E.W. (1994). Fermentation Production of Pyrazines and Terpenoids for Flavour and Fragrances. In: Bioprocess Production of Flavour, Fragrance and Colour, [A. Gabeliman (Ed.)], Wiley, New York, USA: 95-134.
  26. Shankaracharya N.B., Naik P.J., Nagalakshmi, S., Rao, J.M. (1994). Chemical composition and flavour quality of tirphal Zanthoxylum rhetsa. PAFAI Journal. 16(1): 15-21.
  27. Trout, E.S., Hunt, M.C., Johnson, D.E., Clans, J.R., Castner, C.L., Kroff, D.H. (1992). Characteristics of low fat ground beef containing texture modifying ingredients. J Food Sci. 57 (1): 19-24.
  28. Utama, D.T., J. Park, D.S. Kim, E.B. Kim and S.K. Lee (2018). Effect of Ground Chopi (Zanthoxylum piperitum) on Physicochemical Traits and Microbial Community of Chicken Summer Sausage during Manufacture. Korean J Food Sci Anim Resour. 38(5): 936-949.
  29. Verma, A.K., Rajkumar, V., Banerjee, R., Biswas,S. and Das, A.K. (2013). Guava (Psidium guajava L.) powder as an antioxidant dietary fibre in sheep meat nuggets. Asian-Australasian Journal of Animal Sciences. 26(6): 886.
  30. Wardlaw, F.B., McCaskill, L.H. and Acton, J.C. (1973). Effect of postmortem muscle changes on poultry meat loaf properties. Journal of Food Science. 38(3): 421-423.
  31. Witte, V.C., Krause, G.F., Bailey, M.E. (1970). A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. Journal Food Science. 35: 582–585.
  32. Wong, P.K., Yusof, S., Ghazali, H.M. and Man, Y.C. (2002). Physico-chemical characteristics of roselle (Hibiscus sabdariffa L.). Nutrition and Food Science. 38(2): 68-73.
  33. Yamamoto, H. and Ogawa, T. (2002). Antimicrobial activity of perilla seed polyphenols against oral pathogenic bacteria. Biosci. Biotechnology Biochemistry. 66: 921-924.

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