Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 6 (june 2021) : 716-721

Prevalence of Pseudomonas aeruginosa and other Microorganisms from Mastitis Milk and Their Antimicrobial Resistance Pattern

Isha Sekhri, Mudit Chandra, Gurpreet Kaur, Deepti Narang, D.K. Gupta, A.K. Arora
1Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India. 
Cite article:- Sekhri Isha, Chandra Mudit, Kaur Gurpreet, Narang Deepti, Gupta D.K., Arora A.K. (2020). Prevalence of Pseudomonas aeruginosa and other Microorganisms from Mastitis Milk and Their Antimicrobial Resistance Pattern. Indian Journal of Animal Research. 55(6): 716-721. doi: 10.18805/IJAR.B-4112.
Background: Mastitis is a multi-etiological disease. It leads to physical, chemical and bacteriological changes in milk and pathological changes in glandular tissues. In the present study, prevalence of mastitis causing organisms with special reference to Pseudomonas aeruginosa and its antibiotic resistance pattern was studied as Pseudomonas spp. is an important organism in harbouring and transmission of antibiotic resistance in environment.
Methods: One hundred and one milk samples from mastitic cattle and buffaloes from farms in and around Ludhiana and mastitis cases presented in veterinary clinical complex, GADVASU, Ludhiana, Punjab, India were collected from August 2018 till April 2019. All the samples were processed for isolation of bacteria using different media viz., BHI, EMB, MLA, Cetrimide Agar, BP Agar, Edward’s medium and Blood Agar. After the isolation, all the organisms were subjected to cultural, morphological, biochemical tests and PCR for confirmation. All these isolates (except Bacillus spp. and Serratia marcescens) were screened for their antimicrobial resistance against 15 commonly used antibiotics viz., chloramphenicol, erythromycin, tetracycline, amoxycillin, co-trimoxazole, ciprofloxacin, gentamicin, cephalexin, ofloxacin, sparfloxacin, gatifloxacin, teicoplanin, azithromycin, vancomycin and doxycycline.
Result: Out of a total of 101 samples, 86 samples yielded bacterial growth. Out of these 86 samples, 76 (88.37%) samples had a single bacterial growth whereas rest 10 (11.63%) samples had more than one bacterial growth. It was observed after the organisms were subjected to cultural, morphological, biochemical tests and PCR that, a total of 33 (34.02%) Staphylococcus aureus, 24 (24.74%) Escherichia coli, 16 (16.49%) Klebsiella pneumoniae, 5 (5.15%) Pseudomonas aeruginosa, 2 (2.06%) Streptococcus agalactiae, 12 (12.37%) Bacillus spp. and Serratia marcescens 5 (5.15%) were isolated. It was observed that Pseudomonas aeruginosa showed resistance against most of the antibiotics and was sensitive only for ofloxacin (80%) and gentamicin (40%).
  1. Chuanchuen, R., Wannaprasat, W., Ajariyakhajorn., Schweizer, H.P. (2008). Role of the MexXY multidrug efûux pump in moderate aminoglycoside resistance in Pseudomonas aeruginosa isolates from Pseudomonas mastitis. Microbiology and Immunology. 52: 392-398.
  2. Crossman, P.J. and. Hutchinson, I. (1995). Gangrenous mastitis associated with Psuedomonas aeruginosa. Veterinary Record. 136: 548.
  3. Deb, R., Kumar, A., Chakraborty, S., Verma, A.K., Tiwari, R., Dhama, K., Singh, U., Kumar, S. (2013). Trends in Diagnosis and Control of Bovine Mastitis: A Review, Pakistan Journal of Biological Sciences. 16: 1653-1661.
  4. Guidry, A.J. (2007). Mastitis and the immune system of the mammary gland. Lactation. In: Lauson B L. (ed) 2007. The Iowa State University Press Ames, Iowa, USA. pp. 229-262.
  5. Hawari, A.D., Al-Dabbas, F. (2008). Prevalence and distribution of Mastitis Pathogens and their Resistance against antimicrobial agents in dairy Cows in Jordan. American Journal of Animal and Veterinary Science. 3: 36-39.
  6. Heleili, N., Ayachi, A., Melizi, M., Kassah, A.L., Mamache, B. (2012). Prevalence of subclinical bovine mastitis and the in vitro sensitivity of bacterial isolates in Batna Governorate, East of Algeria. Journal of Animal Science Advances. 2: 576-582.
  7. Hogeveen, H., Pyorala, S., Waller, K.P., Hogan, J.S., Lam, T.J.G.M., Oliver, S.P, Schukken, Y.H., Barkema, H.W., Hillerton, J.E. (2011). Current status and future challenges in mastitis research. NMC Annual Meeting Proceedings. pp. 36-48.
  8. Hussein, S.A. (2012). Prevalence and bacterial etiology of subclinical mastitis in dairy cows in Al Sulaimaniyah district. Kufa Journal for Veterinary Medical Sciences. 3: 190-203.
  9. Kour, G., Chandra, M., Kaur, G., Narang, D., Gupta, D.K., Arora, A.K., Sharma, N.S. (2017). Prevalence of mastitis causing organism and their antibiotic resistance pattern in dairy farms. Indian Journal of Dairy Science. 5: 587-592.
  10. Kour, G., Chandra, M., Kaur, G., Narang, D., Gupta, D.K., Arora, A.K. (2018). Genotypic and phenotypic correlation of antimicrobial resistance in bacterial isolates from mastitic milk. Veterinary Immunology and Biotechnology. 1: 24-30.
  11. Kour, G., Chandra, M., Kaur, G., Narang, D., Gupta, D.K. (2020). A simple modification in the DNA extraction process to extract good quality bacterial DNA from milk. Indian Journal of Animal Sciences. 90(4): 29-34.
  12. Kulaste, T. (2019). Development of a Multiplex PCR for the identification of mastitis causing microorganisms. M.V.Sc. Thesis, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
  13. Lambert, P.A. (2002). Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. Journal of Royal Society of Medicine. 95: 22-26.
  14. Lister, P.D., Wolter, D.J., Hanson, N.D. (2009). Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clinical Microbiology Review. 22: 582-610
  15. Markey, B., Leonard, F., Archambault, M., Cullinane, A., Maguire, D. (2013). Clinical Veterinary Microbiology 2nd Edition. (Elsevier), U. S. A.
  16. NDDB (2017-18). National Dairy Development Board Annual Report. http://www.nddb.org/information/stats/milkprodindia. Accessed 18 Aug 2019.
  17. NCCLS (2002). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: approved standard M31-A2. (National Committee for Clinical Laboratory Standards, Wayne, PA, USA).
  18. Park, H.R., Hong, M.K., Hwang, S.Y., Park, Y.K., Kwon, K.H., Yoon, J.W., Shin, S., Kim, J.H. Park, Y.H. (2013). Characterization of Pseudomonas aeruginosa related to bovine mastitis. Acta Veterinaria Hungarica. 62: 1-12.
  19. Radostitis, O.M., Gay, C.C., Blood, D.C., Hinchcliff, K.W. (2006). Mastitis In: Veterinary Medicine. A Text book of the Diseases of cattle, horses, sheep, pig and goats. Philadelphia, USA, W B Saunders Co., (9th Edn). pp. 603-612.
  20. Rahaman, M.M., Islam, M.R., Uddin, M.B., Aktaruzzaman, M. (2010). Prevelance of Subclinical Mastitis in Dairy Cows Reared in Sylhet District of Bangladesh. International Journal of Biological Research. 1: 23-28.
  21. Ramos, J.L. (2004). Pseudomonas. Volume 1 Genomics, Life style and Molecular Architecture. Springer; Heidelberg. pp. 1-7.
  22. Ranjan, R., Gupta, M.K., Singh, K.K. (2011). Study of bovine mastitis in different climatic conditions in Jharkhand, India. Veterinary World. 4: 205-208. 
  23. Ranjan, R., Gupta, M.K., Singh, S., Kumar, S. (2010). Current trend of drug sensitivity in bovine mastitis. Veterinary World. 3: 17-20.
  24. Riekerink, R.O., Barkena, H.W., Kelton, D.F., Scholl, D.T. (2008). Incidence rate of clinical mastitis on Canadian dairy farms. Journal of Dairy Science. 91: 1366-1377.
  25. Riffon, R., Sayasith, H., Khalil, H., Dubreuil, P., Drolet, M., Legace, J. (2001). Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. Journal of Clinical Microbiology. 39: 2584-2589.
  26. Saidi, R., Khelef, D., Kaidi, R. (2013). Subclinical mastitis in cattle in Algeria: Frequency of occurrence and bacteriological isolates. African Journal of Microbiology Research. 84: 1-5.
  27. Sambrook, J., Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbour, Cold Spring Laboratory Press, New York.
  28. Sarangi, A.N., Aggarwal, R., Rahman, Q., Trivedi, N. (2009). Subtractive genomics approach for in silico identification and characterization of novel drug targets in Neisseria meningitides serogroup B. Journal of Computer Science and Systems Biology. 2: 255-258.
  29. Sayed, R.H., Salama, S.S., Soliman, R.T. (2014). Bacteriological evaluation of present situation of mastitis in dairy Cows. Global Veterinaria. 13: 690-695.
  30. Singh, K., Chandra, M., Kaur, G., Narang, D., Gupta, D.K., Arora, A.K., Sharma, N.S. (2019). Development of a multiplex PCR for identification of mastitis causing organisms. Indian Journal of Dairy Science. 72: 177-182.
  31. Singh, R., Sharma, N., Soodan, J.S., Sudhan, N.A. (2005). Etiology and sensitivity of bacterial isolates from sub-clinical mastitis in cattle from Jammu region. SKUAST Jammu Research. 4: 223-224.
  32. Sumathi, B.R., Veeregowda, B.M., Gomes, A.R. (2008). Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Veterinary World. 1: 237-238.

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