Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 6 (june 2021) : 710-715

Identification and Comparative Phylogeny of Sheep and Goat Pox Isolates from Jammu, India

Sankalp Sharma, Nawab Nashiruddullah, Anil Taku, Parimal Roychoudhury, Jafrin Ara Ahmed
1Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Science and Technology of Jammu, RS Pura-181 102, Jammu and Kashmir, India.
Cite article:- Sharma Sankalp, Nashiruddullah Nawab, Taku Anil, Roychoudhury Parimal, Ahmed Ara Jafrin (2020). Identification and Comparative Phylogeny of Sheep and Goat Pox Isolates from Jammu, India. Indian Journal of Animal Research. 55(6): 710-715. doi: 10.18805/IJAR.B-4009.
Background: In view of the limited documentation of sheep and goat pox disease in animals of Jammu region, it was felt necessary to conduct a blanket screening of the small ruminant populations in areas where they are largely reared. 
Methods: The investigation was conducted to study the occurrence of capripox infection amongst small ruminants through clinical survey and confirmatory laboratory diagnosis. PCR amplification of a part of the P32 core protein gene successfully confirmed Capripoxvirus (CaPV) in clinical scab samples from both sheep and goats and from inoculated CAM. Positive amplified samples yielded a predicted 192 bp product. 
Result: Sequencing of the partial P32 gene of one isolate each from sheep (Sheeppoxvirus, SPPV) and goat origin (Goatpoxvirus, GTPV) revealed that each isolate were distinct and showed 97.8% identity with each other. The SPPV clustered with respective sheeppox cluster with 100% homology to with other SPPV strains reported within India and abroad and also to vaccine strains Srinagar and Rumanian-Fanar (RF). The GTPV was closely identical (~98-99%) with other strains from India and abroad with some unique residues of its own. Both the SPPV and GTPV were divergent from Lumpy Skin Disease Virus (LSDV) strains. The sequence of SPPV strain being similar to circulating strains and prevalent vaccines in the country favours a common vaccination strategy. However, specific GTPV vaccine is not available in the country. The circulating CaPV isolates were found to be host specific. The possibility of vaccination failures or disease caused by vaccines itself, or cross transmission between hosts cannot be ruled out and in such instances it becomes difficult to distinguish with natural disease.
  1. Bhanuprakash, V., Indrani, B.K., Hegde, R., Kumar, M.M. and Moorthy, A.R.S. (2004). A classical live attenuated vaccine for sheep pox. Trop. Anim. Health Pro. 36: 307-320. 
  2. Bhanuprakash, V., Venkatesan, G., Balamurugan, V., Hosamani, M., Yogisharadhya, R., Chauhan, R.S., Pande, A., Mondal, B. and Singh, R.K. (2010). Pox outbreaks in sheep and goats at Makhdoom (Uttar Pradesh), India: evidence of sheep pox virus infection in goats. Transbound. Emerg. Dis. 57(5): 375-382.
  3. Buller, R.M., Arif, B.M., Black, D.N., Dumbell, K.R., Esposito, J.J., Lefkowitz, E.J., McFadden, G., Moss, B., Mercer, A.A., Moyer, R.W., Skinner, M.A. and Tripathy, D.N. (2005). Family Poxviridae. In: Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. [Fauquet, C. M., M. A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball, (Eds.)], Elsevier Academic Press, San Diego, pp. 117-133.
  4. Cunningham, C.H. (1973). A Laboratory Guide in Virology. 7th ed., Burgess Publishing Co., Minneapolis, Minnesota.
  5. Heine, H.G., Stevens, M.P., Foord, A.J. and Boyle, D.B. (1999). A Capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. J. Immunol. Methods. 227: 187-196.
  6. Hosamani, M., Mondal, B., Tembhurne, P.A., Bandyopadhyay, S.K., Singh, R.K. and Rasool, T.J. (2004). Differentiation of sheep pox and goat pox viruses by analysis and PCR-RFLP of P32 gene. Virus Genes. 29(1): 73-80.
  7. Ireland, D.C. and Binepal, Y.S. (1998). Improved detection of Capripoxvirus in biopsy samples by PCR. J. Virol. Methods. 74: 1-7.
  8. Mirzaie, K., Barani, S.M. and Bokaie, S. (2015). A review of sheep pox and goat pox: perspective of their control and eradication in Iran. J. Adv. Vet. Anim. Res. 2(4): 373-381.
  9. Parthiban, M., Govindarajan, R., Manoharan, S., Purushothaman, V., Chandran, D.J.N. and Koteeswaran, A. (2005). Comparison of nucleotide sequence analysis of Indian sheeppox virus isolates. Vet. Arhiv. 75: 203-209.
  10. Rajamuthu, S., Parthiban, M., Senthil Kumar, T.M.A., Aruni, W. and Senthil Kumar S. (2009). Nucleotide sequence analysis of the viral envelope protein gene (P32) region of sheep poxvirus from India. Indian J. Anim. Sci. 79(1): 1095-1098.
  11. Ramakrishnan, M.A., Santhamani, R. and Pandey, A.B. (2016). Capripox outbreak in a mixed flock of sheep and goats in India. Transbound. Emerg. Dis. 64(1): 27-30.
  12. Santhamani, R., Yogisharadhya, R., Venkatesan, G., Shivachandra, S.B., Pandey, A.B. and Ramakrishnan, M.A. (2014). Molecular characterization of Indian sheep pox and goat pox viruses based on RPO30 and GPCR genes. Virus Genes. 49(2): 286-291.
  13. Tamura, K., Peterson, D. Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol. Biol. Evol. 28(10):2731-2739.
  14. Van Regenmortel, M.H.V., Fauquet, M. and Bishop, D.H.L. (2000). Virus taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, San Diego: Academic Press.
  15. Venkatesan, G., Balamurugan, V., Yogisharadhya, R., Kumar, A. and Bhanuprakash, V. (2012). Differentiation of sheep pox and goat pox viruses by polymerase chain reaction-restriction fragment length polymorphism. Virol. Sin. 27(6): 353-359.
  16. Yan, X.M., Zhang, Q., Wu, G.H., Li, J. and Zhu, H.X. (2010). Discrimination of Goatpox virus and Sheeppox virus by digestion of p32 gene with Hif I and sequence alignment of GpCR gene. Anim. Husb. Feed Sci. 2: 32-38.
  17. Yogisharadhya, R., Bhanuprakash, V., Hosamani, M., Venkatesan, G., Balamurugan, V., Bora, D.P., Bhanot, V., Prabhu, M. and Singh, R.K. (2011): Comparative efficacy of live replicating sheeppox vaccine strains in ovines. Biologicals. 39: 417-423.

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