Sero-prevalence and Risk Factor Analysis of BoHV-1 in Bovines in Haryana State of India

DOI: 10.18805/ijar.B-4001    | Article Id: B-4001 | Page : 582-587
Citation :- Sero-prevalence and Risk Factor Analysis of BoHV-1 in Bovines in Haryana State of India.Indian Journal of Animal Research.2021.(55):582-587
Sheza Farooq, Aman Kumar, Suman Chaudhary, C.S. Patil, Yogesh Banger, Vipin Khasa, Swati Dahiya, Sushila Maan aman.abt@luvas.edu.in
Address : Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001, Haryana, India.
Submitted Date : 10-02-2020
Accepted Date : 20-06-2020


Background: Bovine herpesvirus-1 (BoHV-1) is an important pathogen of cattle and buffaloes associated with various clinical conditions including infectious bovine rhinotracheitis (IBR) and abortion. To know the status of BoHV-1, a cross-sectional serological study was conducted with the objectives of estimating the apparent prevalence of BoHV-1 and potential risk factor among unorganized cattle and buffalo herds.
Method: A total of 490 serum samples were collected from cattle and buffaloes from all twenty two (22) districts of Haryana from unorganised herd randomly and tested for antibodies against BoHV-1 using ELISA. 
Result: The overall percent sero-prevalence of BoHV-1 was observed as 48.78% however the species wise sero-prevalence was 37.77% in cattle and 62.27% in buffaloes. The overall sero-prevalence was significantly (p < 0.05) associated with species, zone and age of animals. The likelihood of BoHV-1 was significantly higher (2.72 times) in buffaloes (Odds ratio (OR) =2.72, 95% Confidence Interval (CI):1.86; 3.98) than in cattle (OR=1). Eastern zone of the state showed higher (1.52 times, 95% CI: 1.03, 2.26) likelihood of BoHV-1 as compared to western zone (OR=1.00).The aged animals with age³6.5 years (2.96 times), followed by 2.5-4.5 years (2.44 times) and 4.5-6.5 years (1.68 times) showed higher likelihood than younger animals (Age < 2.5 years). Further, it can be concluded that BoHV1 is circulating among livestock population in the state.


Bovines BoHV-1 ELISA Haryana Sero-prevalence


  1. Babu, T.S., Mallick, B.B. and Das, S.K. (1984). Prevalence of infectious bovine rhinotracheitis virus (BHVI) antibodies in bovines. Ind. Vet. J. 61: 195-200.
  2. Chettri S., Ahmed, K., Bora, D.P., Dutta, L.J., Bora, M. and Sarma, D.K. (2015). Reproductive status in Bovine Herpes Virus 1(BHV-1) sero-positive dairy cattle. Ind. J. Ani. Rep. 36: 2.
  3. Deka, D., Varma, R., Maiti, N.K. and Oberoi, M. (2005). Detection of bovine herpesvirus-1 infection in breeding bull semen by vius isolation and polymerase chain reaction. Rev. Sci. et Tech. (Int. Off. Epizootics). 24: 1085-1094.
  4. Dhand, N.K., Singh, G., Sharma, D.R. and Sandhu, K.S. (2002). Seroprevalence of IBR in Punjab. Indian J. Anim. Sci. 72: 850-852. 
  5. Gill, B.S., Sharma, D.R., Kwatra, M.S., Kumar, A. and Gill, J.S. (1987). Seroprevalence of infectious bovine rhinotracheitis in the Punjab State. J. Res. Punjab Agril. Uni. 24: 317-318.
  6. Kampa J., Stahl, K., Moreno-Lopez, J., Chanlun, A., Aiumlamai, S. and Alenius, S. (2004) BVDV and BHV-1 infections in dairy herds in Northern and Northeastern Thailand. Acta Vet. Scand. 45: 181-192.
  7. Khan., O. A. (2004). Seroprevalence of Infectious Bovine Rhinotracheitis in Gujarat State. M.V.Sc. Thesis submitted to G.A.U, Sardarkrushinagar.
  8. Kollannur, J., Davis, S., Radhika, and Chauhan, R.S. (2014). Epidemiological studies on infectious bovine rhinotracheitis (IBR) in different parts of India. Int. J. L. Stock Res. 4: DOI 10.5455/ijlr.20140717052204.
  9. Majumder, S., Ramakrishnan, M.A. and Nandi, S. (2015). Infectious Bovine Rhinotrachitis: An Indian Perspective. Int. J. Cur. Mic. App. Sci. 4: 844-858.
  10. Mallick, B.B. (1986). Importance of bovine herpes virus 1 in India. Proc. Natl. SympOn Current Status of Herpes Virus in Man and Animals. HAU, Hissar. 54-59
  11. Mehrotra, M.L., Rajya, B.S. and Kumar, S. (1976). Infectious bovine rhinotracheitis (IBR) kerato-conjuctivitis in calves: Ind. J. Vet. Path. 1: 70-73.
  12. Nandi, S., Kumar, M., Yadav, V. and Chander, V. (2011). Serological evidences of Bovine Herpesvirus-1 infection in bovines of organized farms in India. Trans. and Emerg. Dis. 58: 105-109.
  13. Nandi, S., Pandey, A.B. Sharma, K. Audarya, S.D. and Chauhan, R.S. (2007). Seroprevalence of infectious bovine rhinotracheitis in cattle of an organized farm by indirect elisa. The Ind. cow: The Sci. and Eco. J. 4: 09729585. 
  14. Renukaradhya, G.J., Rajasekhar, M. and Raghavan, R. (1996). Prevalence of infectious bovine rhinotracheitis in Southern India. Rev. Sci. Tech. Off. Int. Epiz. 15: 1021-1028.
  15. Samal, S.K., Mallick, B.B. and Das, S.K. (1981). Note on the incidence of IBR virus infection among cattle in India. Ind. J. Anim. Sci. 51: 895-897.
  16. Samrath, D. (2009). Seroprevalence of Bovine herpes Virus-1 in Cattle and Buffaloes in Chattisgarh. Thesis submitted to Indira Gandhi Krishi Vishwa Vidyalaya, Raipur.
  17. Samrath, D., Shakya, S., Rawat, N., Gilhare, V.R.,Singh, F. and Khan, F.F. (2016). Seroprevalence of Bovine Herpes Virus Type 1 in Cattle and Buffaloes from Chhattisgarh. J. Ani. Res. 6: 641-644.
  18. Satbige, A.S., Kuotsu, K., Patil, N.A., Kasaralikar, V.R., Bijurkar, R.G. and Sandeep, H. (2018). Molecular detection of bovine herpes virus-1 (BHV-1) infection in cattle in organised and unorganised farm in Bidar, Karnataka. J. Ent. Zoo. Studies. 6: 1258-1260.
  19. Sharma, M., Katoch, R.C., Charanjeet and Dhar, P., (2006) Sero prevalance of IBR among cattle in Himachal Pradesh. Indian Vet. J. 83:1-3.
  20. Singh, R., and Yadav, S., (2010). Seroprevalence of Bovine Herpes Virus -1 in Uttar Pradesh. Haryana Vet. 49: 54-55.
  21. Sulochana, S., Pillai, R.M., Nair, G.K, and Abdulla, P.K, (1981) Prevalence of Infectious bovine rhinotracheitis in Kerala. In Third annual convention and National symposium on reproductive infection of livestock and their economic significance, IAVMI, Tirupati. pp. 31.
  22. Thakur, V., Kumar, M. and Rathish, R.L. (2017) Seroprevalence of bovine herpesvirus-1 antibodies in bovines in five districts of Uttarakhand. Vet. World. EISSN. 2231-0916.
  23. Trangadia, B.J., Rana, S.K., Mukherjee, F. and Srinivasan, V.A. (2010). Prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in India. Trop. Ani. H. Prod. 42: 203-207.
  24. Vaid, J., Lalkrishna and Kaushal, R.S. (1991). Seroprevalence of animal diseases in Himachal Pradesh. Ind. Vet. J. 68: 705-707.

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