Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 55 issue 5 (may 2021) : 493-497

Enhancement of Developmental Competence of Immature Oocytes Supplemented with Growth Factors in Culture Media 

Sonia B. Umdor, M. Karunakaran, D.K. Mandal, A. Santra, Subrata K. Das
1Animal Biotechnology Lab, ICAR-National Dairy Research Institute, Eastern Regional Station, Kalyani-741 235, West Bengal, India.
Cite article:- Umdor B. Sonia, Karunakaran M., Mandal D.K., Santra A., Das K. Subrata (2021). Enhancement of Developmental Competence of Immature Oocytes Supplemented with Growth Factors in Culture Media. Indian Journal of Animal Research. 55(5): 493-497. doi: 10.18805/IJAR.B-3994.

ABSTRACT

Background: In vitro embryo production is a valuable tool for understanding early mammalian development, therapeutic applications, excellent source for research in the field of developmental biology and production of valuable animals. The purpose of this study is to improve the production of in vitro cattle embryos using fibroblast and platelet derived growth factor as media supplement. 
Methods: Ovaries were collected from local abattoir in 0.9% saline (30-35°C) supplemented with antibiotics. Cumulus oocyte complexes were aspirated, washed 5-6 times and placed in maturation media supplemented with growth factors and cultured in 5% CO2 incubator at 38.5°C with maximum humidity. After 24 h oocytes were co-incubated with in vitro capacitated sperms for fertilization for 15-18 h and then presumptive zygotes were cultured for embryo development. Cleavage was observed after 40-42 h and embryos were co-cultured with oviductal cells for 7-9 days. 
Result: The highest cleavage and blastocyst formation rates were 55.93 ± 4.75, 57.06 ± 4.78, 51.24 ± 4.12 and 3.26 ±1.53, 2.42 ± 1.02, 2.70 ± 1.17 in FGF (1ng ml-1), PDGF (10 ng ml-1) and in combination of FGF and PDGF (1ng ml-1 each) respectively. It can be concluded that PDGF (10 ng ml-1) enhanced cleavage rate and FGF (1ng ml-1) enhanced blastocyst formation rate.

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