Enhancement of Developmental Competence of Immature Oocytes Supplemented with Growth Factors in Culture Media 

DOI: 10.18805/IJAR.B-3994    | Article Id: B-3994 | Page : 493-497
Citation :- Enhancement of Developmental Competence of Immature Oocytes Supplemented with Growth Factors in Culture Media.Indian Journal Of Animal Research.2021.(55):493-497
Sonia B. Umdor, M. Karunakaran, D.K. Mandal, A. Santra, Subrata K. Das subratakdas1@gmail.com
Address : Animal Biotechnology Lab, ICAR-National Dairy Research Institute, Eastern Regional Station, Kalyani-741 235, West Bengal, India.
Submitted Date : 3-02-2020
Accepted Date : 8-02-2021

Abstract

Background: In vitro embryo production is a valuable tool for understanding early mammalian development, therapeutic applications, excellent source for research in the field of developmental biology and production of valuable animals. The purpose of this study is to improve the production of in vitro cattle embryos using fibroblast and platelet derived growth factor as media supplement. 
Methods: Ovaries were collected from local abattoir in 0.9% saline (30-35°C) supplemented with antibiotics. Cumulus oocyte complexes were aspirated, washed 5-6 times and placed in maturation media supplemented with growth factors and cultured in 5% CO2 incubator at 38.5°C with maximum humidity. After 24 h oocytes were co-incubated with in vitro capacitated sperms for fertilization for 15-18 h and then presumptive zygotes were cultured for embryo development. Cleavage was observed after 40-42 h and embryos were co-cultured with oviductal cells for 7-9 days. 
Result: The highest cleavage and blastocyst formation rates were 55.93 ± 4.75, 57.06 ± 4.78, 51.24 ± 4.12 and 3.26 ±1.53, 2.42 ± 1.02, 2.70 ± 1.17 in FGF (1ng ml-1), PDGF (10 ng ml-1) and in combination of FGF and PDGF (1ng ml-1 each) respectively. It can be concluded that PDGF (10 ng ml-1) enhanced cleavage rate and FGF (1ng ml-1) enhanced blastocyst formation rate.

Keywords

Cattle Embryo FGF IVF Oocyte PDGF

References

  1. Borah, D. and Biswas, R.K. (2020). Effect of additives in medium on in-vitro maturation of goat oocytes. Indian Journal of Animal Research. 54: 11-15.
  2. Brackett, B.G. and Oliphant, G. (1975). Capacitation of rabbit spermatozoa in vitro. Biology of Reproduction. 12: 260-274.
  3. Brackett, B.G., Younis, A.I. and Fayrer-Hosken, R.A. (1989). Enhanced viability after in vitro fertilization of bovine oocytes matured in vitro with high concentrations of luteinizing hormone. Fertility and Sterility. 52(2): 319-324.
  4. Das, S.K., Chauhan, M.S., Palta, P., Katiyar, P.K. and Madan, M.L. (1996). Replacement of fetal bovine serum and FSH with buffalo follicular fluid in in vitro maturation of buffalo oocytes. Theriogenology. 45(1): 245.
  5. Das, S.K., Sharma, A.K., Mahapatra, S.K., Chatterjee, A., Bhatia, V. and Mohanty, A.K. (2013). Purification of cattle oviduct specific proteins and their effect on in-vitro embryo development. Livestock Science. 152(1): 88-93.
  6. de Souza-Fabjan, J.M., Panneau, B., Duffard, N., Locatelli, Y., de Figueiredo, J.R., Freitas, V.J. and Mermillod, P. (2014). In vitro production of small ruminant embryos: late improvements and further research. Theriogenology. 81 (9): 1149-1162.
  7. Diogenes, M.N., Guimaraes, A.L.S., Leme, L.O. and Dode, M.A.N. (2017). Bovine in vitro embryo production: the effects of fibroblast growth factor 10 (FGF10). Journal of Assisted Reproduction and Genetics. 34(3): 383-390.
  8. Fiammetta, B. (2015). Embryo Production. In: Reproduction in livestock. (ÓEncyclopedia of Life Support Systems (EOLSS), p. 203-217. 
  9. Fields, S.D., Hansen, P.T. and Ealy, A.D. (2011). Fibroblast growth factor requirements for in vitro development of bovine embryos. Theriogenology. 75(8): 1466-75.
  10. Gasparrini, B., Boccia, L., Marchandise, J., Di. Palo, R., George, F., Donnay, I. and Zicarelli, L. (2006). Enrichment of in vitro maturation medium for buffalo (Bubalus bubalis) oocytes with thiol compounds: effects of cystine on glutathione synthesis and embryo development. Theriogenology. 65 (2): 275-287.
  11. Herrick, J.R., Behboodi, E., Memili, E., Blash, S., Echelard, Y. and Krisher, R.L. (2004). Effect of macromolecule supplementation during in vitro maturation of goat oocytes on developmental potential. Molecular Reproduction and Development. 69: 338-346.
  12. Kumar, R., Chandra, P., Konyak, P., Karunakaran, M., Santra, A. and Das, S.K. (2020). In vitro development of preimplantation caprine embryo using cryopreserved black bengal buck semen. Indian Journal of Animal Research. 54(10): 1210-1214.
  13. Lim, J.M. and Hansel, W. (2000). Exogeneous substances affecting development of in vitro-derived bovine embryos before and after embryonic genome activation. Theriogenology. 53(5): 1081-1091.
  14. Madan, M.L., Chuhan, M.S., Singla, S.K. and Manik, R.S. (1994). Pregnancies established from water buffalo (Bubalus bubalis) blastocysts derived from in vitro matured, in vitro fertilizated oocytes and co-cultured with cumulus and oviductul cells. Theriogenology. 42: 591-600.
  15. Paramio, M.T. and Izquierdo, D. (2014). Current status of in vitro embryo production in sheep and goats. Reproduction in Domestic Animals. 49(4): 37-48.
  16. Rajesh, K., Swathi, B., Aruna Kumari, G. and Shanmugam, M. (2020). Effect of kisspeptin on in vitro maturation of buffalo oocytes. Indian Journal of Animal Research. 54: 1218-1222.
  17. Reyes-Perea, A.D., Aguila, L., Smith, L., Diaw, M. and Guerrero Netro, H.M. (2019). Fibroblast growth factor 10 enhances equine oocyte maturation and blastocyst formation in vitro. Biomedical Journal of Scientific and Technical Reearch. 21(1): 15509-15514.
  18. Thibodeaux, J.K., Del Vecchio, R.P. and Hansel, W. (1993). Role of platelet-derived growth factor in development of in vitro matured and in vitro fertilized bovine embryos. Journal of Reproduction and Fertility. 98: 61-66.
  19. Totey, S.M., Pawshe, C.H. and Singh, G.P. (1993). In vitro maturation and fertilization of buffalo oocytes (Bubalis bubalis): effects of media, hormones and sera. Theriogenology. 39: 1153-1171.
  20. Vikash, C., Mishra, A. and TaruSharma, G. (2012). Effect of growth factors (epidermal growth factor, platelet derived growth factor and insulin-like growth factor-1) on buffalo embryos produced in vitro. Indian Journal of Animal Sciences. 82 (12): 1510-1514.
  21. Yang, B.K., Yang, X. and Foote, R.H. (1993). Effect of growth factors on morula and blastocyst development of in vitro matured and in vitro fertilized bovine oocytes. Theriogenology. 40: 521-530.

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