Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.43

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 55 issue 5 (may 2021) : 542-549

Histological Study on the Effects of Allogenic Bone Marrow Derived Mesenchymal Stem Cells Along with Local Insulin Therapy on Osteosynthesis in Gap Defects of Radius in Diabetic Rabbit Model

Sonu Jaiswal, Narendra Singh Jadon, Priyanka Pandey, Deepti Bodh, Sara Kaushal
1Department of Surgery and Radiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263 145, Uttarakhand, India.
Cite article:- Jaiswal Sonu, Jadon Singh Narendra, Pandey Priyanka, Bodh Deepti, Kaushal Sara (2020). Histological Study on the Effects of Allogenic Bone Marrow Derived Mesenchymal Stem Cells Along with Local Insulin Therapy on Osteosynthesis in Gap Defects of Radius in Diabetic Rabbit Model. Indian Journal of Animal Research. 55(5): 542-549. doi: 10.18805/ijar.B-3993.

ABSTRACT

Background: Diabetes mellitus impairs fracture healing causing bone repair abnormality which is insulin dependent. Exogenous insulin can accelerate fracture healing in diabetes by promoting bone remodeling and bone formation. Mesenchymal stem cell loaded scaffolds having osteoinductive and osteoconductive properties, may be used as an alternative for autogenous bone grafting. The current study aimed to investigate the efficacy of cultured allogenic bone marrow derived mesenchymal stem cell implantation with insulin therapy for increasing osteosynthesis in bone gap defects in experimentally induced diabetic rabbits.
Methods: Thirty six, New Zealand White rabbits were divided into four groups: A, B, C and D. Diabetes was induced experimentally in all rabbits, except group A. A 5 mm bone defect was created in right radii of all rabbits and following treatment was given: hydroxyapatite granules (group A and B), hydroxyapatite granules and insulin therapy (group C), cultured allogenic BMMSCs in hydroxyapatite scaffold and insulin therapy (group D). Histologic evaluation was performed on days 30, 60 and 90.
Result: Combined therapy of allogenic BMMSCs in hydroxyapatite scaffold along with local insulin (group D) provided best healing response followed by healthy control (group A), locally insulin treated group (group C) and diabetic control (group B).

REFERENCES

  1. Atilgan, S., Yaman, F., Yilmaz, U., Gurgun, B. and Unlu, G. (2007). An experimental comparison of the effects of calcium sulfate particles and â-TCP/HA granules on osteogenesis in internal bone cavities. Biotechnology and Biotechnological Equipment. 21: 205-210.
  2. Brar, R. S., Sandhu, H. S. and Singh, A. (2002). Histopathological techniques. In: Veterinary Clinical Diagnosis by Laboratory Method. Kalyani Publishers, New Delhi. pp: 186-196.
  3. Breitbart, E. A., Meade, S., Azad, V., Yeh, S., Al-Zube, L., Lee, Y. S., Benevenia, J., Arinzeh, T. L. and Lin, S. S. (2010). Mesenchymal stem cells accelerate bone allograft incorporation in the presence of diabetes mellitus. Journal of Orthopaedic Research. 28: 942-949.
  4. Culling, C. F. A. (1963). Handbook of Histopathological Techniques, Butterworth and Co. Ltd., London, pp. 52.
  5. Dedania, J., Borzio, R., Paglia, D., Breitbart, E.A., Mitchell, A., Vaidya, S. and Wey, A. (2011). Role of local insulin augmentation upon allograft incorporation in a rat femoral defect model. Journal of Orthopaedic Research. 29: 92-99.
  6. Diniz, S.F., Amorim, F.P., Cavalcante-Neto, F.F., Bocca, A.L., Batista, A.C., Simm, G.E. and Silva, T.A. (2008). Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture. Brazilian Journal of Medical and Biological Research. 41: 373-379.
  7. Frank, O., Heim, M., Jacob, M., Barbero, A., Schafer, D., Bendik, I., Dick,W., Heberer, M. and Martin, I. (2002). Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro. Journal of Cellular Biochemistry, 85: 737-746.
  8. Honczarenko, M., Le, Y., Swierkowski, M., Ghiran, I., Glodek, A.M. and Silberstein, L.E. (2006). Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors. Stem Cells. 24: 1030-1041.
  9. Irwin, R., Lin, H. V., Motyl, K. J. and Mc Cabe, L.R. (2006). Normal bonedensity obtained in the absence of insulin receptor expression in bone. Endocrinology. 147: 5760-5767.
  10. Jang, B. J., Byeon, Y. E., Lim, J. H., Ryu, H. H., Kim, W. H., Koyama, Y., Kikuchi, M., Kang, K. S. and Kweon, O. K. (2008). Implantation of canine umbilical cord blood-derived MSCs mixed with â TCP enhances osteogenesis in bone defect model dogs. Journal of Veterinary Science. 9: 387-393.
  11. Jiang, X.., Zou, S., Ye, B., Zhu, S., Liu, Y. and Hu, J. (2010). âFGF-modified BMMSCs enhance bone regeneration following distraction osteogenesis in rabbits. Bone. 46: 1156-1161.
  12. Jung, D.I., Ha, J., Kang, B.T., Kim, J.W., Quan, F.S., Lee, J.H., Woo, E.J. and Park, H. (2009). A comparison of autologous and allogenic BMMSCs transplantation in canine spinal cord injury. Journal of the Neurological Sciences. 285: 67-77.
  13. Lane, J.M. and Sandhu, H.S. (1987). Current approach to experimental bone grafting. Orthopedic Clinics of North America. 18: 213-225.
  14. Lu, H., Kraut, D., Gerstenfeld, L. and Graves, D. (2003). Diabetes interfereswith the bone formation by affecting the expression of transcription factors that regulate osteoblast differentiation. Endocrinology. 144: 346-352.
  15. Luna, L.G. (1992). Routine staining procedure. In: Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 4th Edn., McGraw hill Company, New York. pp: 35.
  16. Niemeyer, P., Szalay, K., Luginbuh, R., Sudkamp, N.P. and Kasten, P. (2010). Transplantation of human MSCs in a non-autogenous setting for bone regeneration in a rabbit critical-size defect model. Acta Biomaterialia. 6: 900-908.
  17. Paglia, D.N., Mason, K., Breitbart, E.A., Vaidya, S., Graves, D. T.,O’Connor, J.P. and Lin, S.S. (2011). Effects of diabetes on bone homeostasis, regeneration, and the role of insulin in bone. US Musculoskeletal Review. 6: 50-55.
  18. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods. 8th ed, Lowa State University Press, Ames, pp. 217-235.
  19. Southwood, L.L., Frisbie, D.D., Kawcak, C.E. and Mcilwraith, C.W. (2005). Delivery of growth factors using gene therapy to enhance bone healing. Veterinary Surgery. 33: 565-578.
  20. Wang, J., Wan R, Mo, Y., Zhang, Q., Sherwood, L.C. and Chien, S. (2010). Creating a long-term diabetic rabbit model. Experimental Diabetes Research. 1-10.
  21. Wetzler, C., Kampfer, H., Stallmeyer, B., Pfeilschifter, J. and Frank, S. (2000). Large and sustained induction of chemokines during impaired wound healing in the genetically diabetic mouse prolonged persistence of neutrophils and macrophages during the late phase of repair. Journal of Investigative Dermatology. 115: 245-253.
  22. Wu, Y., Chen, L. and Scott, P. G. (2007). Mesenchymal stem cells enhancewound healing through differentiation and angiogenesis. Stem Cells. 25: 2648-2659. 
  23. Yu, M., Zhou, W., Song, Y., Yu, F., Li, D., Na, S., Zou, G., Zhai, M., Xie, C. (2011) Development of mesenchymal stem cell-implant complexes bycultured cells sheet enhances osseointegration in type 2 diabetic ratmodel. Bone. 49: 387-394.
  24. Zhou, G., Liu, W., Cui, L., Wang, X., Liu, T. and Cao, Y. (2006). Repair of porcine articular osteochondral defects in non-weight bearing areas with autologous bone marrow stromal cells. Tissue Engineering. 12: 3209-3221. 
  25. Zhu, W., Zhang, X., Wang, D., Lu ,W., Ou, Y., Han, Y., Zhou, K., Liu, H., Fen, W., Peng, L. C. and Zeng, H.Y. (2010). Experimental studies on the conduction function of nano-hydroxyapatite artificial bone. Micro and Nano Letters. 5: 19-27.
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)