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H Gene-based Molecular Characterization of Field Isolates of Canine Distemper Virus from Cases of Canine Gastroenteritis

DOI: 10.18805/ijar.B-3989    | Article Id: B-3989 | Page : 561-567
Citation :- H Gene-based Molecular Characterization of Field Isolates of Canine Distemper Virus from Cases of Canine Gastroenteritis.Indian Journal of Animal Research.2021.(55):561-567
Haritha Kodi, Kalyani Putty, Vishweshwar Kumar Ganji, B. Bhagyalakshmi, Y. Narasimha Reddy, K. Satish, M. Gnana Prakash  kalyaniputty@gmail.com
Address : College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar-500 030, Hyderabad, India.
Submitted Date : 25-01-2020
Accepted Date : 15-05-2020

Abstract

Background: Canine distemper virus (CDV) is one of the important causes for canine gastroenteritis. Its genome codes for six structural proteins N, P, M, F, H and L, of which H is important for viral pathogenesis. Availability of CDV molecular epidemiological data is sparse. Our study reports for the first time, isolation of enteric CDV from India and its molecular epidemiology. 
Methods: We isolated the circulating wild type CDV from cases of canine gastroenteritis recorded during 2019 in India. Partial H gene of the isolates was amplified and phylogeny was reconstructed with other isolates from the NCBI database using MUSCLE from MEGA7.
Result: The multiple sequence alignment of the partial H gene of circulating CDV with reference isolates revealed 88-95% nucleotide identity, and 83-91% amino acid identity along with the presence of Nde1 restriction site at position 1571-1576 that is typical of wild type CDV isolates. Interestingly, phylogenetic analysis revealed that the circulating CDV were grouped with three different lineages i.e., South America-2, Asia-4, and Asia-5/India-1 and distantly related with the vaccine strains. Our study strengthens the need for the development of a relevant vaccine comprising of circulating viral strains for effective vaccination strategies.

Keywords

Canine distemper virus H gene Phylogenetic analysis

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