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Virulence Gene Pattern of Pasteurella multocida Isolates of Buffalo in Association to Capsule Biosynthesis Genes

DOI: 10.18805/IJAR.B-3977    | Article Id: B-3977 | Page : 469-473
Citation :- Virulence Gene Pattern of Pasteurella multocida Isolates of Buffalo in Association to Capsule Biosynthesis Genes.Indian Journal of Animal Research.2021.(55):469-473
N. Sujatha, K. Lakshmi Kavitha, K.V. Subramanyam, T. Srinivasa Rao, R.N. Ramani Pushpa kommalapatiin@yahoo.co.in
Address : Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram-521 101, Sri Venkateswara Veterinary University, Andhra Pradesh, India.
Submitted Date : 6-01-2020
Accepted Date : 21-08-2020

Abstract

Background: Pasteurella multocida is the causative agent of many economically important diseases in a wide range of hosts. The mechanisms by which these bacteria can invade the mucosa, evade innate immunity and cause systemic disease are slowly being elucidated. Many key virulence factors are yet to be identified, including those required for initial attachment and invasion of host cells and for persistence in a relatively nutrient poor and hostile environment. This has led to intensive research to understand host adaptation mechanisms and virulence factors in order to develop effective vaccines. 
Methods: The present study was carried out to know the distribution of virulence genes viz., haemoglobin binding proteins (hgbA and hgbB), outer membrane protein (ompH), fimbrial antigen (ptfA), filamentous haemagglutinin (pfhA) and transferrin binding protein (tbpA) by PCR in P. multocida CapA isolates from apparently healthy or carrier animals and CapB isolates from field Haemorrhagic septicemia (HS) cases to monitor the epidemiological associations of virulence genes in Cap A and Cap B isolates.
Result: The study revealed that all the six virulence associated genes were present in Cap B isolates. None of the Cap A isolates harboured tbpA and pfhA genes. These two genes were closely related to serotype B causing Haemorrhagic septicemia and were epidemiologically associated with disease status.

Keywords

Buffaloes Capsular typing Haemorrhagic septicemia Pasteurella multocida PCR Virulence factors

References

  1. Aski, H.S. and Tabatabaei, M. (2016). Occurrence of virulence-associated genes in Pasteurella multocida isolates obtained from different hosts. Microbial pathogenesis. 96: 52-57.
  2. Cox, A.J., Hunt, M.L., Boyce, J.D., Adler, B. (2003). Functional characterization of HgbB, a new hemoglobin binding protein of Pasteurella multocida. Microbiology Pathology. 34: 287-296.
  3. Doughty, S.W., Ruffolo, C.G., Adler, B. (2000). The type 4 fimbrial subunit gene of Pasteurella multocida. Veterinary Microbiology. 72: 79-90.
  4. Ewers, C., Lubke Becker, A., Becker, A., Bethe, A., Kieblings, S., Filter, M., Wiefer, H. (2006). Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status. Veterinary Microbiology. 114: 304-317.
  5. Harper, M., Boyce, J. D., Adler, B. (2006). Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiology Letter. 265: 1-10.
  6. Holland, J., Parsons, T. R., Hasan, A. A., Cook, S. M., Stevenson, P., Griffiths, E., Williams, P. (1996). Conservation and antigenic cross-reactivity of the transferrin-binding proteins of Haemophilus influenzae, Actinobacillus pleuropneumoniae and Neisseria meningitidis. Microbiology. 142: 3505-3513.
  7. Jamali, H., Rezagholipour, M., Fallah, S., Dadrasnia, A., Chelliah, S., Ritadevi, V., Kelvin, S.C.W., Ismail, S. (2014). Prevalence, characterization and antibiotic resistance of Pasteurella multocida isolated from bovine respiratory infection. The Veterinary Journal. 202: 381-383.
  8. Katsuda, K., Hoshinoo, K., Ueno, Y., Kohmoto, M., Mikami, O. (2013). Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves. Veterinary Microbiology. 167: 737-741. 
  9. Khamesipour, F., Momtaz, H., Mamoreh, M.A. (2014). Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran. Frontiers in Microbiology. 5: 1-8.
  10. Ogunnariwo, J.A. and Schryvers, A.B. (2001). Characterization of a novel transferrin receptor in bovine strains of Pasteurella multocida. Journal of Bacteriology. 183: 890-896.
  11. Potter, A.A., Schryvers, A.B., Ogunnariwo, J.A., Hutchins, W.A., Lo, R.Y., Watts, T. (1999). Protective capacity of the Pasteurella haemolytica transferrin-binding proteins TbpA and TbpB in cattle. Microbiology Pathology. 27: 197-206.
  12. Ragavendhar, K., Thangavelu, A., John kirubaharan, J., Samuel masilamoni Ronald, B., Ramesh Kumar, P., Daniel joy chandran, N. (2015). Prevalence of virulence associated genes in Pasteurella multocida isolates from Tamilnadu. Indian journal of animal sciences. 85: 1289-1292.
  13. Ruffolo, C.G., Tennent, J.M., Michalski, W.P., Adler, B. (1997). Identification, purification and characterization of the type 4 fimbriae of Pasteurella multocida. Infection and Immununity. 65: 339-343. 
  14. Sarangi, L.N., Priyadarshini, A., Santhosh, K., Prasad, T., Santhosh Kumar, G., Nagaleekar, V.K., Vijendra Pal Singh. (2014). Virulence genotyping of Pasteurella multocida isolated from multiple hosts from India. The scientific world journal. 1-10. 
  15. Shayegh, J., Atashpaz, S., Salehi, T.Z., Hajazi, M.S. (2010). Potential of Pasteurella multocida isolated from healthy and diseased cattle and buffaloes in induction of diseases. Bulletin of the Veterinary Institute in Pulway. 54: 299-304.
  16. Somashekar, S.H., Veeregowda., Suryanaryana, V.V.S., Basawaraj, A., Isloor, S., Rathnamma, D. (2016). Rapid typing of Pasteurella multocida isolates from haemorrhagic septicaemia cases by multiplex PCR for their virulence. Indian Journal of Biotechnology. 15: 124-126.
  17. SPSS Inc. Released 2008. SPSS Statistics for Windows, Version 17.0. Chicago: SPSS Inc.
  18. Sujatha, N., Lakshmi Kavitha, K., Subramanyam, K.V., Srinivasa Rao, T., Srivani, M. (2018). Isolation and molecular characterization of Pasteurella multocida isolates of buffaloes. Indian Journal of Comparative microbiology immunology and infectious diseases. 39: 1-6.
  19. Veken, J.W., Oudega, B., Luirink, J., DeGraaf, F.K. (1994). Binding of bovine transferrin by Pasteurella multocida serotype B:2,5 a strain which causes haemorrhagic septicaemia in buffalo and cattle. Microbiology Letter. 115: 253-258.

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