Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 55 issue 4 (april 2021) : 469-473

Virulence Gene Pattern of Pasteurella multocida Isolates of Buffalo in Association to Capsule Biosynthesis Genes

N. Sujatha, K. Lakshmi Kavitha, K.V. Subramanyam, T. Srinivasa Rao, R.N. Ramani Pushpa
1Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram-521 101, Sri Venkateswara Veterinary University, Andhra Pradesh, India.
Cite article:- Sujatha N., Kavitha Lakshmi K., Subramanyam K.V., Rao Srinivasa T., Pushpa Ramani R.N. (2020). Virulence Gene Pattern of Pasteurella multocida Isolates of Buffalo in Association to Capsule Biosynthesis Genes. Indian Journal of Animal Research. 55(4): 469-473. doi: 10.18805/IJAR.B-3977.

ABSTRACT

Background: Pasteurella multocida is the causative agent of many economically important diseases in a wide range of hosts. The mechanisms by which these bacteria can invade the mucosa, evade innate immunity and cause systemic disease are slowly being elucidated. Many key virulence factors are yet to be identified, including those required for initial attachment and invasion of host cells and for persistence in a relatively nutrient poor and hostile environment. This has led to intensive research to understand host adaptation mechanisms and virulence factors in order to develop effective vaccines. 
Methods: The present study was carried out to know the distribution of virulence genes viz., haemoglobin binding proteins (hgbA and hgbB), outer membrane protein (ompH), fimbrial antigen (ptfA), filamentous haemagglutinin (pfhA) and transferrin binding protein (tbpA) by PCR in P. multocida CapA isolates from apparently healthy or carrier animals and CapB isolates from field Haemorrhagic septicemia (HS) cases to monitor the epidemiological associations of virulence genes in Cap A and Cap B isolates.
Result: The study revealed that all the six virulence associated genes were present in Cap B isolates. None of the Cap A isolates harboured tbpA and pfhA genes. These two genes were closely related to serotype B causing Haemorrhagic septicemia and were epidemiologically associated with disease status.

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