Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

DOI: 10.18805/ijar.B-3976    | Article Id: B-3976 | Page : 439-444
Citation :- Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits.Indian Journal Of Animal Research.2021.(55):439-444
Rashmi, Rekhapathak, P. Tamilmahan, Amarpal, H.P. Aithal, P. Kinjavdekar, A.M. Pawde, T.B. Sivanarayanan, Shanthi Jayalekshmi drrashmisingh16@gmail.com
Address : Department of Veterinary Surgery, Veterinary College and Research Institute, Orathanadu-614 625, Tamil Nadu, India.
Submitted Date : 2-01-2020
Accepted Date : 1-04-2020

Abstract

The present work was assessed the restoration of segmental bone gap defect in rabbits by application of composite bone grafts. Composite bone grafts were prepared by seeding of rabbit foetal osteoblast on the bovine acellular cancellous bone matrix and evaluated in the segmental gap defect of 20 mm in rabbits. Thirty six adult New Zealand White rabbits of either sex were allotted to three groups of 12 each. Autograft (group A), Acellular cancellous bone matrix (group B) and composite graft seeded with fetal osteoblasts (group C) were implanted in the defects. Radiography, gross observations and histopathology at different intervals were done to evaluate healing. It was concluded that the seeded foetal osteoblasts in the composite grafts augment regeneration of the new bone leading to better integration of graft in host in comparison to bovine acellular cancellous bone matrix graft.

Keywords

Acellular bone matrix Composite graft Foetal rabbit osteoblast Segmental gap defect.

References

  1. Badylak SF and Gilbert TW (2008). Immune response to biologic scaffold materials. Seminars in Immunology. 20(2):109-116.
  2. Bauermeister A (1961). Treatment of cysts, tumors and inflammatory processes of the bone with the “Kiel graft”. Bruns Beitrage Zur Klinischen Chirurgie. 203: 287–316.
  3. Breitbart EA, Meade S, Azad V, Yeh S, Al–Zube L, Lee YS, Benevenia J, Arinazeh T L and Lin, SS (2010). Mesenchymal stem cells accelerate bone allograft incorporation in the presence of diabetes mellitus. Journal of Orthopaedic Research. 28(7): 942-949.
  4. Burwell RG (1985). The function of bone marrow in the incorporation of a bone graft. Clinical Orthopaedic and Related Research. 200: 125-141.
  5. Caporali EHG, Sheila CR, Morceli J, Taga R, Mauro JO, Tania GM, Maria C, Mamprim J and Mariana AC (2006). Assessment of bovine biomaterials containing bone morphogenetic proteins bound to absorbable hydroxyapatite in rabbit segmental bone defects. Acta Cirurgica Brasileira. 21(6): 367.
  6. Choi IH, Kim HG, Kim NS and Sasaki N (1996). Effectiveness of freeze-dried bone grafts on the non–union fracture of dogs. Korean Journal of Veterinary Research. 36: 495-511.
  7. Costantino PD and Friedman CD (1994). Synthetic bone graft substitutes. Otolaryngologic Clinics of North America. 27 (5): 1037-1074.
  8. Dhandayuthapani B, Yoshida Y, Maekawa T and Kumar DS (2011). Polymeric Scaffolds in tissue engineering application: A review. International Journal of Polymer Science. 1-19.
  9. Gomes C, Paz AH, Bonilla AT, Correa A, Cavalcante RL, Veiga D, Colome LM, Foerstnow LP, Contesini EA and Cirne-Lima EO (2011). Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 63(4): 836-843.
  10. Heiple KG, Goldberg VM, Powell AE and Zika JM (1987). Biology of cancelluous bone grafts.  The Orthopaedic Clinics of North America. 18(2): 179-185.
  11. Hur JW, Yoon SJ and Ryu SY (2012). Comparison of the bone healing capacity of autogenous bone, demineralized freeze dried bone allograft and collagen sponge in repairing rabbit cranial defects. Journal of the Korean Association of Oral and Maxillofacial Surgeons. 38(4): 221–230.
  12. Kaveh K, Ibrahim R, Ibrahim TA and Bakar MZ.A (2010). Bone MarrowSeeded Bone Graft Versus Bone Graft; Compact Bone Critical Sized Defect Healing Pattern in Rabbit. Journal of Animal and Veterinary Advances. 9: 1588-1596.
  13. Kneser U, Schaefer DJ and Polykandriotis E (2006). Tissue engineering of bone: the reconstructive surgeon’s point of view. Journal of Cellular and Molecular Medicine. 10: 7-19.
  14. Lane JM and Sandhu HS (1987). Current approach to experimental bone grafting. The Orthopaedic Clinics of North America. 18(2): 213–225.
  15. Lieberman JR, Daluiski A and Einhorn TA (2002). The role of growth factors in there pair of bone. Journal of Bone and Joint Surgery. 84:1032-1044.
  16. Luna, LG (1968). Manual of histologic staining methods of the Armed Forces Institute of pathology. 3rd ed. McGraw–hill, New York.
  17. Pathak R, Amarpal, Tiwari AK, Kurade NP and Amarnath (2012). Decellularization of buffalo bone to prepare bone scaffolds for effective bone tissue engineering. Journal of Cell and Tissue Research. 12(3): 3291-3295.
  18. Rashmi, Pathak R, Amarpal, Aithal HP, Kinjavdekar P, Pawde AM, Tiwari AK, Sangeetha P, Tamilmahan P, Manzoor AB. (2017). Evaluation of tissue-engineered bone constructs using rabbit fetal osteoblasts on acellular bovine cancellous bone matrix. Veterinary World. 10(2): 163-169.
  19. Siegel S and Castellan NJ (1988). Non-parametric statistics for the behavioural sciences. 2nd ed, McGraw Hill Book Company, New York, pp. 399.
  20. Tamilmahan P (2013). Development of acellular osseous xenograft for bone tissue engineering in rabbits. Thesis, M.V.Sc. Deemed University, Indian Veterinary Research Institute, Izatnagar, India.
  21. Trentz OA, Hoerstrup SP, Sun LK, Bestmann L, Platz A and Trent OL (2003). Osteoblasts response to allogenic and xenogenic solvent dehydrated cancellous bone in vitro. Biomaterials. 24: 3417–1426.
  22. Ved N and Haller JO (2002). Periosteal reaction with normal appearing underlying bone: a child abuse mimicker. Emergency Radiology. 9: 278-282.
  23. Wallington EA (1972). Histological Methods for Bone. Butterworths Laboratory Aids, London. Butterworths.
  24. Wang L, Fan H, Zhang ZY, Lou AJ, Pei GX, Jiang S, Mud TW, Qin JJ, Chen SY and Jin D (2010). Osteogenesis and angiogenesis of tissue-engineered bone constructed by prevascularized beta-tricalcium phosphate scaffold and mesenchymal stem cells. Biomaterials. 31: 9452-9461.
  25. Yildirim M, Spiekermann H, Handt S and Edelhoff D (2001). Maxillary sinus augmentation with the xenograft Bio–Oss® and autogenous intraoral bone for qualitative improvement of the implant site: a histologic and histomorphometric clinical study in humans. International Journal of Oral and Maxillofacial Implants. 16(1):23-33.

Global Footprints