Cloning and Expression of Fasciola gigantica Cathepsin-B Recombinant Proteins

DOI: 10.18805/ijar.B-3959    | Article Id: B-3959 | Page : 333-339
Citation :- Cloning and Expression of Fasciola gigantica Cathepsin-B Recombinant Proteins.Indian Journal Of Animal Research.2021.(55):333-339
Andleeb Aftab, Savita Bisen, Rohit Lall, Shobha Yadav, Silamparasan, M, O.K. Raina rainaok@rediffmail.com
Address : Divion of Parasitology,  Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh, India. 
Submitted Date : 30-11-2019
Accepted Date : 17-03-2020

Abstract

Fasciola gigantica cathepsin (cysteine) proteases are potential diagnostic antigens for animal and human fasciolosis. These include cathepsin-L proteases that have been exploited in the diagnosis of animal fasciolosis. However, no scientific data on the diagnostic potential of F. gigantica cathepsin B proteases is available. Therefore, three recombinant antigens of F. gigantica viz. cathepsin (cat) B-1, cat B-2 and cat B-3 were expressed in prokaryotic expression system. The recombinant antigens were purified under denaturing conditions by Nickel affinity chromatography and an optimal level of the recombinant proteins was obtained. These recombinant proteins will further be evaluated for their potential in the early prepatent diagnosis of F. gigantica infection in domestic ruminants.

Keywords

Cathepsin B Fasciola gigantica Recombinant antigen Serodiagnosis

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