Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 55 issue 3 (march 2021) : 287-294

Effect of Fish Silage Supplemented Diets on Growth and Health Status of Pangas Catfish, Pangasianodon hypophthalmus Fry

Injeela Khan, Vaneet Inder Kaur, Surjya Narayan Datta
1Department of Aquaculture, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
Cite article:- Khan Injeela, Kaur Inder Vaneet, Datta Narayan Surjya (2020). Effect of Fish Silage Supplemented Diets on Growth and Health Status of Pangas Catfish, Pangasianodon hypophthalmus Fry. Indian Journal of Animal Research. 55(3): 287-294. doi: 10.18805/ijar.B-3954.
Background: Freshwater Asian catfish, Pangasianodon hypophthalmus (pangas) is one of the fastest growing exotic species throughout world including India. Most recently, it has been considered as a potential candidate species for carp diversification especially in northern states of India including Punjab and Haryana. In Pangas culture, fish meal is used as primary protein source pertaining to its excellent amino acid profile, palatability and high nutritive value. However, due to continuously rising cost, limited availability and quality variations of fish meal, there is need to find out less expensive alternative quality protein sources. Among these, fish silage is easy to prepare product with high nutritional value and shelf life.  In this view, the experimental study was conducted to evaluate the possibility of replacing fish meal with fish silage supplemented diets on growth and health status of Pangas catfish.
Methods: Indoor study was conducted in FRP pools (1.5×1×0.75m) for 120 days (July – October 2018) to evaluate the effect of acid fermented fish silage (prepared from fish waste collected from local fish market of Ludhiana, Punjab) for Pangas catfish fry. To study the effect of fish silage, four experimental diets were formulated by replacing fishmeal @ 50% and 100% and mixture of soybean and groundnut meal @ 25% and 50% levels from control diet along with one reference diet without any animal protein source (neither fish meal nor fish silage)
Result: The overall results of present study revealed that fish silage can be incorporated (100% replacement of fish meal with fish silage) in the diet of Pangas catfish fry (P. hypophthalmus) with improved growth and feed utilization along with positive influence on health status of fish in terms of general haematology, biochemistry and anti-oxidant status.
  1. Abedin, M.J., Bapary, M.A.J., Rasul, M.G., Majumdar, B.C., Haque, M.M. (2017). Water quality parameters of some Pangasius ponds at Trishal Upazila, Mymensingh, Bangladesh. European J. Biotech. Biosci. 5(2): 29-35.
  2. Agouz, H.M. and Tonsy, H.D. (2003). Evaluation of whole crayfish meal (Procambrus clarkii) as partial or complete replacement of fishmeal protein in polyculture commercial diets. Egyptian J. Nutr. and Feeds. 6(Special Issue): 315-330.
  3. AOAC (Association of Official Analytical Chemists). (1995). Official Methods of Analysis. Association of Official Analytical Chemists Incorporation. Arlington, USA. p 684. 
  4. Alceste, C.C. and Jory, D.E. (2000). TILAPIA Alternative protein sources in Tilapia feed formulation. Aquaculture Magazine. 26(4): 3.
  5. Al-Azab, A.A. (2005). Effect of partial and complete replacement of fish meal with locally produced shrimp meal on growth performance of Nile tilapia (Oreochromis niloticus). Egyptian J. Nutr. Feeds. 8(Special Issue): 1145-1156.
  6. APHA. (2005). Standard Methods for the Examination of Water and Wastewater. 21st Edn. American Public Health Association, Washington, D.C.
  7. Beerli, E.L., Beerli, K.M. and Logato, P.V.R. (2004). Silagemacida de resíduos de truta (Onchoryncus mykiss) com a utilização de ácidomuriático. Ciência Agrotecnológica 28: 195-98.
  8. Boitai, S.S., Babu, L.K., Pati, P.K., Pradhan, C.R., Tanuja, S., Kumar, A. and Panda, A.K. (2018). Effect of dietary incorporation of fish silage on growth performance, serum biochemical parameters and carcass characteristics of broiler chicken. Indian J. Ani. Res. 52(7): 1005-1009.
  9. Datta, S.N., Singh, A. and Mandal, A. (2018). Effect of different dietary protein sources on hematological parameters of striped catfish Pangasianodon hypophthalmus. J. Ento. Zoo. Studies. 6(2): 3198-3202.
  10. FAO. (2003). Animal feed resources information system. http:// 
  11. Fagbenro, O.A. and Jauncey, K. (1994). Chemical and nutritional quality of dried fermented fish silage and their nutritive value for tilapia (Oreochromis niloticus). Ani. Feed Sci. Tech. 45: 167-176.
  12. Fagbenro, O.A. and Bello-Olusoji, O.A. (1997). Preparation, nutrient composition and digestibility of fermented shrimp head silage. Food Chem. 60: 489-493.
  13. Fossati, G., Colnaghi, M.I., Della Porta, G., Cascinelli, N. and Veronsesi, V. (1971). Cellular and Humoral Immunity against Malignant Melanoma. Intl. J. Cancer 8: 344-55.
  14. Giri, S.S., Sahoo, S.K., Sahu, A.K. and Mukhopadhyay, P.K. (2000). Nutrient digestibility and intestine enzyme activity of Clarias batrachus (Linn) juveniles fed on dried fish and chicken viscera incorporated diets. Biores. Technol. 71: 97-101.
  15. Gornall, A.G., Bardawill, C.J. and David, M.M. (1949). Determination of serum proteins by means of the biuret reaction. The J. Biol. Chem. 177: 751-766.
  16. Goda, A.M., El-Haroun, E.R. and Chowdhury, A.M. (2007). Effect of totally or partially replacing fish meal by alternative protein sources on growth of African catfish Clarias gariepinus (Burchell, 1822) reared in concrete tanks. Aquaculture Res. 38: 279-287.
  17. Gouveia, A.I.R. (1992). The use of poultry by-product and hydrolyze feather meal as a feed for rainbow trout Oncorhynchus mykiss. Publicacoes Do instituto Do Zoologia, 227: 24.
  18. Gui, D., liu, W., Shao, X. and Xu, W. (2010). Effects of different dietary levels of cottonseed meal proteinhydrolysate on growth, digestibility, body composition and serum biochemical indices in crucian carp (Carassius auratus gibelio). Ani. Feed Sci. Tech. 156: 112-120.
  19. Habte-Tsion, H.M., Liu, B., Ge, X., Pan, L. and Chen, R. (2013). Effects of dietary protein level on growth performance, muscle composition, blood composition and digestive enzyme activity of wuchang bream (Megalobrama amblycephala) fry. Israeli J. Aquacult. Bamidgeh. 65:69.
  20. Haider, S.M., Ashraf, M., Azmat, H., Khalique, A., Javaid, A., Atique, U., Zia, M., Iqbal, J.K. and Akram, S. (2016). J. Appl. Ani. Res. 44(1): 158-164.
  21. Hedayati, A. and Tarkhani, R. (2014) Hematological and gill histopathological changes in iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) exposed to sub lethal diazinon and deltamethrin Concentrations. Fish Physiol. Biochem. 40: 715-720.
  22. Kamei, M., Sahu, B., Raman, S., Nanda, S., Choudhury, D. and Dorothy, M.S. (2018). Use of fish silage based blended protein source for replacement of fish meal in Thai-pangas diet. Intl. J. Curr. Microbiol. Appl. Sci. 7(10): 2949-2961.
  23. Khan, N., Atique, U., Ashraf, M., Mustafa, A., Mughal, M.S., Rasool, F., Azmat, H., Tayyab, M. and Iqbal, K.J. (2018). Effect of various protein feeds on the growth, body composition, hematology and endogenous enzymes of catfish (Pangasius hypophthalmus). Pakistan J. Zoo. 13:112-119.
  24. Lemaire, P., Drai, P., Mathieu, A., Lemaire, S., Carrie‘re, S., Giudicelli, J. and Lafaurie, M. (1991). Changes with different diets in plasma enzymes (GOT, GPT, LDH, ALT) and plasma lipids (cholesterol, triglyc-erides) of sea-bass (Dicentrarchus labrax). Aquaculture. 93:63–75.
  25. Lin, S. and Lou, L. (2011). Effects of different levels of soybean meal inclusion in replacement for fishmeal on growth, digestive enzymes and transaminase activities in practical diets for juvenile tilapia, Oreochromis niloticus×O. aureus. Ani. Feed Sci. Technol. 168: 80-87.
  26. Middleton, T.F., Ferket, P.R., Boyd, L.C., Daniels, H.V. and Gallagher, M.L. (2001). An evaluation of co-extruded poultry silage and culled jewel sweet potatoes as a feed ingredient for hybrid tilapia (Oreochromis niloticus × O. mossambicus). Aquaculture. 198: 269-280.
  27. Millamena, O.M. (2002). Replacement of fish meal by animal by-    product meals in a practical diet for growout culture of grouper, Epinephelus coioides. Aquaculture. 204:75-84.
  28. Mukherjee, K.L. (1988). Medical Laboratory Technology. Tata McGraw Hill Publishing Company Ltd, New Delhi 1: 2425-2443.
  29. New, M.B. and Wijkstrom, U.N. (2002). Use of fishmeal and fish oil in aquafeeds: Further thoughts on the fishmeal trap. F.A.O fisheries circular No. 975. Food and Agriculture Organization of the United Nations. Rome.
  30. Nishikimi, M., Rao, N.A. and Yagi, K. (1972). The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem. and Biophysical Res. Commun. 46: 849-864. 
  31. Oetterer, M. (2002). Industrialização do pescado cultivado. Livraria e Editora Agropecuária, Guaíba: RS-Brasil. 
  32. Placer Z.A., Cushman, L.L. and Johnson B.C. (1966). Estimation of product of lipid peroxidation (Malonyl dialdehyde) in biochemical systems. Annals of Biochem. 16: 359-64.
  33. Plascencia-Jatomea, M., Olvera-Novoa, M.A., Arredondo-Figueroa, J.L., Hall, G.M. and Shirai, K.(2002). Feasibility of fishmeal replacement by shrimp head silage protein hydrolysate in Nile tilapia (Oreochromis niloticus) diets. J. Sci. Food Agricult. 82(7): 753-759.
  34. Qi-you, X.U, Chan, Li., Ping, Y., Hong X.U. and Chang-gan, W. (2008). Effects of partial replacement of fishmeal with soy protein isolated and meat bone meal on growth and non-specific immunity in rainbow trout. J. Dalian Fish. Uni. 1: 8-12. 
  35. Roeschlau, P., Bernt, E. and Gruber, W. 1974. Enzymatic determina- -tion of total cholesterol in serum. J. Clinical Chem. Clinical Biochem. 12:226. 
  36. Sahli, T. (1962). Textbook of chemical pathology. In: Williams and Williams C. [Scward E (Ed.)] Baltimore, USA. pp. 35.
  37. Salah al-din, S.H.A. (1995). Master of science studies on unconventional rations in feeding of Nile catfish (Calarias lazera) Fac, Agric. AI - Azhar University, Cairo.
  38. Salah, M.N., Al-Noor, S.S. and Jasim, B.M. (2014). Effects of fish meal replacement with fish biosilage on some haemato- -logical and biochemical parameters in common carp, Cyprinus carpio fingerlings. Intl. J. Res. Fish. Aquacult. ISSN 2277-7729.
  39. Singh, A.H. and Lakra, W.S. (2012). Culture of Pangasianodon hypopthalmus into India: impacts and present scenario. Pakistan J. Biol. Sci. 15(1): 19-26.
  40. Santhosh, B. and Singh, N.P. (2007). Guidelines for water quality 
  41. management for fish culture in Tripura, ICAR Research Complex for NEH Region, Tripura Center, Publication no. 29.
  42. Soltan, M.A. and Tharwat, A.A. (2006). Use of fish silage for partial or complete replacement of fishmeal in diets of nile tilapia (Oreochromis niloticus) and African catfish (Clarius gariepinus). Egyptian J. Nutr. Feeds. 9(2):299-314.
  43. Tanuja, S., Kumar, A. and Nayak S.K. (2017) Effect of dietary intake of acid ensiled fish waste on the growth, feed utilization, hematology and serum biochemistry of rohu Labeo rohita, (Hamilton, 1822) fingerlings. Indian J. Ani. Res. 51(3): 501-505.
  44. Tanuja, S., Kumar, A., Nayak, S. K., Behera, S. K. and Sarkar, A. (2018). Effect of dietary supplementation of acid ensiled fish waste on production performance, egg quality and serum biochemistry in layer Japanese quail (Coturnix coturnix japonica). Indian J. Ani. Res. 52(5): 740-743.
  45. Wagner, T. and Congleton, J.L. (2004). Blood-chemistry correlates of nutritional condition, tissue damage, and stress in migrating juvenile Chinook salmon Oncorhynchus tshawytscha. Canadian J. Fish. Aqua. Sci. 61: 1066-1074.
  46. Wassawa, J., Tang, J. and Gu, X. (2007). Utilization of fish processing by-products in the Gelatin Industry. Food Reviews Intl. 23(2):159-174.
  47. Wassef, E.A., Sweilam, M.A. and Attalah, R.F. (2003). The use of fermented fish silage as a replacement for fish meal in Nile tilapia (Oreochromis niloticus) diets. Egyptian J. Nutr. Feed. 6(Special Issue):357-370.
  48. Wiegertjes, G.F., Stet, R.J.M., Parmentier, H.K. and Van, M.W.B. (1996). Immunogenetics of disease resistance in fish: A Comparative Approach. Develop. Comp. Immunol. 20: 365-381.
  49. Yones, A.M.M. and Metwalli, A.A. (2015). Effects of fish meal substitution with poultry by-product meal on growth performance, nutrients utilization and blood contents of juvenile Nile Tilapia (Oreochromis niloticus). Aquacul. Res. Devel. 6: 389.

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