Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 55 issue 2 (february 2021) : 193-198

Acute Phase Protein as Biomarker for Diagnosis of Sub-Clinical Mastitis in Cross-Bred Cows

Deepa Chouhan, Ranjit Aich, Ravindra Kumar Jain, Daljeet Chhabra
1Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Mhow-453 446, Indore, Madhya Pradesh, India. 
Cite article:- Chouhan Deepa, Aich Ranjit, Jain Kumar Ravindra, Chhabra Daljeet (2020). Acute Phase Protein as Biomarker for Diagnosis of Sub-Clinical Mastitis in Cross-Bred Cows. Indian Journal of Animal Research. 55(2): 193-198. doi: 10.18805/ijar.B-3943.
The present study was carried out to investigate the role of acute phase proteins [Serum amyloid A (SAA) and milk amyloid A (MAA)] for diagnosis of sub-clinical mastitis in cross-bred cows. The study was conducted on 40 apparently healthy cross-bred cows, in the mid-lactation period and were equally divided into four groups [Group-I (Bacteriologically negative milk samples, California mastitis test (CMT) negative), Group-II (Bacteriologically negative with SCC £200,000 cells/ml milk, CMT positive), Group-III (Bacteriologically negative with SCC ³200,000 £500,000 cells/ml milk, CMT positive) and Group-IV (Bacteriologically positive with SCC ³200,000 £500,000 cells/ml milk, CMT positive)]. On bacteriological examination of 57 milk samples, 27(47.36%) were found positive and 30 (52.63%) were found negative. A highly significant (P<0.01) differences in the mean values of SCC were observed between groups except group I and II and group III and IV. Highly significant (P<0.01) differences in mean values of SAA were reported between all groups except group I and II. Highly significant (P<0.01) differences in mean values of MAA were observed between all groups except group III and IV. Results of the correlation matrix revealed strong positive correlations (P<0.01) of SCC with SAA and MAA; and between SAA and MAA. The proposed cutoff points for SCC, SAA and MAA were >200,000 cells/ml, 74 µg/ml and 10 µg/ml, respectively for the diagnosis of sub-clinical mastitis with high sensitivity (90% to 100%). The area under curve of the SAA was larger than those of SCC and MAA, which suggests that the SAA test was more accurate than SCC and MAA for the diagnosis of sub-clinical mastitis.
  1. Akerstedt, M., Waller, K.P. and Sternesjo, A. (2007). Haptoglobin and serum amyloid-A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples. Journal of Dairy Research. 74: 198-203.
  2. Ayano, A.A., Hiriko, F., Simyalew, A.M. and Yohannes, A. (2013). Prevalence of subclinical mastitis in lactating cows in selected commercial dairy farms of Holeta district. Journal of Veterinary Medicine and Animal Health. 5(3): 67-72.
  3. Bradley, A.J. (2002). Bovine mastitis: an evolving disease. Indian Veterinary Journal. 164: 116-128.
  4. Chahar, A. (2001). Studies on some epidemiological and diagnostic aspects of bovine sub-clinical and clinical mastitis. Ph.D. Thesis, Rajasthan Agricultural University, Bikaner, Rajasthan.
  5. Dohoo, I.R. and Meek, A.H. (1982). Somatic cell count in bovine milk. Canadian Veterinary Journal. 23: 119-125.
  6. Eckersall, P.D., Young, F.J., Mc-Comb, C., Hogarth, C.J., Safi, S., Weber, A., McDonald, T., Nolan, A.M. and Fitzpatrick, J.L. (2001). Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Veterinary Record. 148: 35-41.
  7. Farag, V.M.E.M., Abd-El-Moaty, A.M., Ibrahim, N.A., Atwa, S.M. and El-Beskawy, M.A.N. (2017). Serum amyloid A4 and ceruloplasmin evaluated mastitic cattle with Escherichia coli or Staphylococcus aureus including resistant genes. Journal of Bioanalysis and Biomedicine. 9(3): 132-136.
  8. Haghkhah, M., Nazifi, S. and Jahromi, A.G. (2010). Evaluation of milk haptoglobin and amyloid A in high producing dairy cattle with clinical and sub-clinical mastitis. Comparative Clinical Pathology. 19: 547-552. 
  9. Horadagoda, N.U., Knox, K.M., Gibbs, H.A., Reid, S.W., Horadagoda, A., Edwards, S.E. and Eckersall, P.D. (1999). Acute phase proteins in cattle: discrimination between acute and chronic inflammation. Veterinary Record. 144(16): 437-441.
  10. Hussein, H.A., EI-Razik, A. A. E. H. A. K., Gomaa, A.M., Elbayoumy, M.K., Abde­lrahman, K.A. and Hosein, H.I. (2018). Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle. Veterinary World. 11(1): 34-41.
  11. Jaeger, S., Virchow, F., Torgerson, P.R., Bischoff, M., Biner, B., Hartnack, S. and Ruegg, S.R. (2017). Test characteristics of milk amyloid A ELISA, somatic cell count and bacterio- -logical culture for detection of intramammary pathogens that cause subclinical mastitis. Journal of Dairy Science. 100: 7419–7426.
  12. Kumar, P., Sharma, A., Sindhu, N. and Deora, A. (2014). Acute phase proteins as indicators of inflammation in streptococcal and staphylococcal mastitis in buffaloes. Haryana Veterinarian. 53(1): 46-49.
  13. Malinowski, E. and Klossowska, A. (2002). Diagnostyka zakazen i zapalen wymienia. National Veterinary Research Institute, Pulawy, Poland.
  14. Mcdermott, M.P., Erb, H.N. and Natzke, R.P. (1982). Predictability by somatic cell counts related to prevalence of intramammary infections within herds. Journal of Dairy Science. 65: 1535-    1539.
  15. O’Mahoney, M.C., Healy, A.M., Harte, D., Walshe, K.G., Torgerson, P.R. and Doherty, M.L. (2006). Milk amyloid A: correlation with cellular indices of mammary inflammation in cows with normal and raised serum amyloid A. Research in Veterinary Science. 80: 155-161.
  16. Ondiek, J.O., Ogore, P.B., Shakala, E.K. and Kaburu, G.M. (2013). Prevalence of bovine mastitis, its therapeutics and control in Tatton Agriculture Park, Egerton University, Njoro Kenya. The Journal of Agricultural Science. 2(1): 15-20.
  17. Persson, Y., Nyman, A.K. and Grondlund-Andersson, U. (2011). Etiology and antimicrobial susceptibility of Udder pathogens form cases of subclinical mastitis in dairy cows in Sweden. Acta Veterinaria Scandinavica. 53(36): 1-8.
  18. Pyorala, S. (2003). Indicator of inflammation in the diagnosis of mastitis. Veterinary Research. 34: 565-578.
  19. Safi, S., Khoshvaghti, A., Jafarzadeh, S.R., Bolourchi, M. and Nowrouzian, I. (2009). Acute phase proteins in the diagnosis of bovine subclinical mastitis. Veterinary Clinical Pathology. 38: 471-476.
  20. Schalm, O. W., Carroll, E. J. and Jain, N. C. (1971). Number and types of somatic cells in normal and mastitic milk: Bovine mastitis. 1st Edn., Lea and Febiger. Philadelphia, pp 94- 127. 
  21. Schalm, O.W. and Noorlander, D.O. (1957). Experiments and observations leading to development of the California mastitis test. Journal of American Veterinary Medical Association. 130(5): 199-204.
  22. Singh, M., Sharma, A., Sharma R., Mittal, D., Yadav, P. and Charaya, G. (2015). Estimation of acute phase protein as early biomarkers of buffalo sub-clinical mastitis. Asian Journal of Animal Veterinary Advances. 10(12): 894-902.
  23. Skrzypek, R., Wtowski, J. and Fahr, R.D. (2004). Factor affecting somatic cell count in cow bulk tank milk - a case study from Poland. Journal of Veterinary Medicine A Physiology Pathology Clinical Medicine. 51(3): 127-131.
  24. Szczubial, M., Dabrowski, R., Kankofer, M., Bochniarz, M. and Albera, E. (2008). Concentration of serum amyloid A and activity of ceruloplasmin in milk from cows with clinical and subclinical mastitis. Bulletin of the Veterinary Institute in Pulawy. 52: 391-395.

Editorial Board

View all (0)