Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 50 issue 3 (june 2016) : 366-369

A963G single nucleotide variant of BMP15 is not common bio-marker for fecundity in goat

Guang-Xin E, Yong-Fu Huang, Jian-Ning He, Wei-Wei Ni, Yong-Ju Zhao*
1<p>College of Animal Science and Technology, Chongqing Key Laboratory of Forage &amp; Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing 400716, China.</p>
Cite article:- E Guang-Xin, Huang Yong-Fu, He Jian-Ning, Ni Wei-Wei, Zhao* Yong-Ju (2016). A963G single nucleotide variant of BMP15 is not commonbio-marker for fecundity in goat . Indian Journal of Animal Research. 50(3): 366-369. doi: 10.18805/ijar.9363.

Bone morphogenetic protein 15 (BMP15) is crucial factor for ovulation as well as for increasing litter size. In the present investigation efforts had been carried out  to assess the genetic variations in Exon 2 region of BMP15 in goat, using polymerase chain reaction single strand conformation polymorphism (PCR-SSCP)  sequencing methods and cooperated frequency distribution to discuss its possibility of related fecundity. Across the 144samples from six breeds were identified in the A963G location of BMP15 using PCR-SSCP and sequences technology. A963A genotype was the most frequent (85.4%) and G963G was the least frequent with a  frequency of 4.2% and A963G is 10.4%.. It  revealed non significant different between high and low fecundity breed. Therefore, this single nucleotide variant is not common Bio-Marker for fecundity in Goat.

  1. Ahlawat S, Sharma R, Maitra A, Roy M. and Tantia MS. (2014) Designing, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec genes. Meta Gene. 2:439-49. doi: 10.1016/    j.mgene.2014.05.004.

  2. Ahlawat S, Sharma R and Maitra A. (2013) Screening of indigenous goats for prolificacy associated DNA markers of sheep. GENE. 517:128-31. doi: 10.1016/j.gene.2012.12.015.

  3. An X, Hou J, Gao T, Lei Y, Li G, Song Y, Wang J and Cao B. (2015a). Single-nucleotide polymorphisms g.151435C&gt;T and g.173057T&gt;C in PRLR gene regulated by bta-miR-302a are associated with litter size in goats. Theriogenology. 83:1477-1483

  4. An X, Ma T, Hou J, Fang F, Han P, Yan Y, Zhao H, Song Y, Wang J and Cao B. (2013) Association analysis between variants in KISS1 gene and litter size in goats. BMC Genet. 14:63. doi: 10.1186/1471-2156-14-63.

  5. An XP, Hou JX, Gao TY, Lei YN, Song YX, Wang JG and Cao BY. (2015b) Association analysis between variants in KITLG gene and litter size in goats. GENE. 558:126-30. doi: 10.1016/j.gene.2014.12.058.

  6. An XP, Hou JX,Li G, Song YX, Wang JG, Chen QJ, Cui YH, Wang YF and Cao BY. (2012) Polymorphism identification in the goat KITLG gene and association analysis with litter size. Anim Genet. 43:104-7. doi: 10.1111/j.1365-    2052.2011.02219.x. 

  7. Celestino JJ, Lima-Verde IB, Bruno JB, Matos MH, Chaves RN, Saraiva MV, Silva CM, Faustino LR, Rossetto R, Lopes CA, Donato MA, Peixoto CA, Campello CC, Silva JR and Figueiredo JR (2011) Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles. Mol Cell Endocrinol. 338:1-9. doi: 10.1016/j.mce.2011.02.007.

  8. Cerra C, Oliver J, Roberts SA, Horne G, Newman WG4 and Mohiyiddeen L. (2014) A single nucleotide polymorphism of bone morphogenic protein-15 is not associated with ovarian reserve or response to ovarian stimulation. Hum Reprod. 29:2832-7. doi: 10.1093/humrep/deu264.

  9. China National Commission of Animal Genetic Resources. (2011). Animal Genetic Resources in China: Sheep and Goats. Beijing: Chinese Agricultural Press.

  10. Chu, M.X., Jiao, C.L., He, Y.Q., Wang, J.Y., Liu, Z.H. and Chen, G.H., 2007. Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Anim. Biotechnol. 18: 263-274.

  11. Davis, G.H., Balkrishnan, L., Ross, I.K., Wilson, T., Galloway, S.M., Lumsden, B.M., Hanrahan, J.P., Mullen, M., Mao, X.Z., Wang, G.L., Zhao, Z.S., Zeng, Y.Q., Robinson, J.J., Mavrogenis, A.P., Papachristoforou, C., Peter, C., Baumung, R., Cardyn, Baujenane, I., Cockett, N.E., Eythorsdottir, E., Arranz, J.J. and Notter, D.R., (2006). Investigation of the Booroola (Fec B) and Inverdale (Fec X I) mutation in 21 prolific breeds and strains of sheep samples in 13 countries. Anim. Reprod. Sci. 92: 87-96.

  12. Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L, Tosser-Klopp G, Mulsant P, Nowak Z, Drobik W, Martyniuk E, Bodin L. (2014) Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. PLoS Genet. 9(4):e1003482. doi: 10.1371/    journal.pgen.1003482.

  13. Dong CH and Du LX (2010) Polymorphism Analysis of BMP15 Gene Related to Reproduction Trait of Goat Breeds in Shandong Province. Chinese Journal of Zoology, 45:59 - 69

  14. Fonseca DJ, Ortega-Recalde O, Esteban-Perez C, Moreno-Ortiz H, Patiño LC, Bermúdez OM2, Ortiz AM, Restrepo CM, Lucena E and Laissue P. (2014) BMP15 c.-9C>G promoter sequence variant may contribute to the cause of non-    syndromic premature ovarian failure. Reprod Biomed Online. 29:627-33. doi: 10.1016/j.rbmo.2014.07.018.

  15. Feng T, Zhao YZ, Chu MX, Di R, Zhang YJ and Fang L. (2009) Bone Morphogenetic Protein 15 as a Candidate Gene for Prolificacy of Jining Grey Goat. Chinese Journal of Animal and Veterinary Science. (S1): 1-7

  16. Hanrahan PJ, Gregan SM., Mulsant P, Mullen M, Davis GH, Powell R and Galloway SM. (2004) Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in cambridge and belclare sheep (Ovis aries). Biol. Reprod. 70: 900-909.

  17. Hou JX, An XP, Han P, Peng JY and Cao BY. (2015) Two missense mutations in exon 9 of caprine PRLR gene were associated with litter size. Anim Genet. 46:87-90. doi: 10.1111/age.12223.

  18. Hua GH, Chen SL, Ai JT and Yang LG. (2008) None of polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Anim Reprod Sci. 108:279-86.

  19. Hua GH, Chen SL, Yu JN, Cai KL, Wu CJ, Li QL, Zhang CY, Liang AX, Han L, Geng LY, Shen Z, Xu DQ and Yang LG (2009) Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci. 81:391-395

  20. Jiao CL, Chu, M.X., Wang, J.Y., Fang, L. and Ye, S., (2007). Cloning and sequence analysis of exon 2 of BMP15 gene in Jining Grey goats. Chin. Anim. Vet. Sci. 34: 52-55.

  21. Maitra A, Sharma R, Ahlawat S, Tantia MS, Roy M and Prakash V. (2014). Association analysis of polymorphisms in caprine KiSS1 gene with reproductive traits. Anim Reprod Sci. 151: (1-2):71-7. doi: 10.1016/    j.anireprosci.2014.09.013.

  22. Persani L, Rossetti R, Di Pasquale E, Cacciatore C and Fabre S. (2014) The fundamental role of bone morphogenetic protein 15 in ovarian function and its involvement in female fertility disorders. Hum Reprod Update. 20:869-    83. doi: 10.1093/humupd/dmu036.

  23. Pramod RK, Sharma SK, Singhi A, Pan S and Mitra A. (2013) Differential ovarian morphometry and follicular expression of BMP15, GDF9 and BMPR1B influence the prolificacy in goat. Reprod Domest Anim. 48:803-9. doi: 10.1111/    rda.12165. 

  24. Sambrook J and Russel, DW, (2001) Molecular Cloning: A Laboratory Manual. third ed. Cold spring Harbour Laboratory Press, New York.

  25. Sharma R, Ahlawat S, Maitra A, Roy M, Mandakmale S and Tantia MS. (2013) Polymorphism of BMP4 gene in Indian goat breeds differing in prolificacy. GENE, 532 :140-5. doi: 10.1016/j.gene.2013.08.086.

  26. Souza C.J, MacDougall C, Campbell BK, McNeilly AS and Baird DT. (2001). The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B (BMPR1B) gene. J. Endocrinol. 169, R1-R6.

  27. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739.

  28. Wang W, Liu S, Li F, Pan X, Li C, Zhang X, Ma Y, La Y, Xi R and Li T.(2015) Polymorphisms of the Ovine BMPR-IB, BMP-15 and FSHR and their associations with litter size in two Chinese indigenous sheep breeds. Int J Mol Sci. 16:11385-97. doi: 10.3390/ijms160511385.

  29. Zamani P, Nadri S, Saffaripour R, Ahmadi A, Dashti F and Abdoli R. (2015) A new mutation in exon 2 of the bone morphogenetic protein 15 gene is associated with increase in prolificacy of Mehraban and Lori sheep. Trop Anim Health Prod. 47(5):855-60. doi: 10.1007/s11250-015-0799-2.


Editorial Board

View all (0)