Evaluation of TBARS, SOD and GPx in Saliva during Pregnancy of Murrah Buffaloes Maintained under Isohyet Conditions

DOI: 10.18805/ijar.B-3907    | Article Id: B-3907 | Page : 1481-1485
Citation :- Evaluation of TBARS, SOD and GPx in Saliva during Pregnancy of Murrah Buffaloes Maintained under Isohyet Conditions.Indian Journal Of Animal Research.2020.(54):1481-1485
Valluri Paramesh, Thavitiki Prasada Rao, K. Padmaja, B. Punyakumari, Varra Manasa, T.V. Sai Kumar and Kutty Kumar prasadmvsc@gmail.com
Address :
Department of Veterinary Biochemistry, College of Veterinary Science, Sri Venkateswara Veterinary University, Proddatur-516 360, Andhra Pradesh, India. 
Submitted Date : 16-08-2019
Accepted Date : 21-11-2019

Abstract

The objective of the present study was to assess the oxidative status in saliva samples during pregnancy by measuring salivary antioxidant enzymes and oxidative stress marker in Murrah buffaloes. We considered saliva as an alternative biological sample because of its non-invasiveness and also from farmer’s perspective. In our study 20 saliva samples each ten were collected from non-pregnant and pregnant Murrah buffaloes maintained under agro climatic regions receiving equal rain fall and laid on equal elevation to avoid geospatial differences. The mean saliva TBARS concentration was significantly (P≤ 0.05) higher (2.59±0.31 µmoles/L) whereas SOD and GPx levels were significantly (P≤ 0.05) lower during pregnancy when compared to non-pregnant Murrah buffaloes. In our study, a significant increase in concentration of TBARS in saliva of pregnant Murrah buffaloes was observed whereas the activity of antioxidant enzymes SOD and GPx were significantly decreased in saliva during pregnancy. 

Keywords

GPx Murrah buffaloes Pregnancy Saliva SOD TBARS

References

  1. Adela, P., Zinveliu, D., Pop, R. andrei, S. and Kiss, E. (2006). Antioxidant status in dairy cows during lactation. Bulgarian Journal of Agricultural Science. 21: 655-661.
  2. Agarwal, A., Gupta, S. and Sharma, R. (2005).Oxidative stress and its implications in female infertility – A clinician’s perspective. Reprodutive Bio Medicine. 11(5): 641-650.
  3. Arikan, S., Konukoglu, D., Arýkan, C., Akcay, T. and Davas, I. (2001).Lipid peroxidation and antioxidant status in maternal and cord blood.Gynecology Obstetrics Investigation.51: 145-149.
  4. Atmaca, G. (2004). Antioxidant effects of sulfur-containing amino acids. Yonsei Medical Journal. 45: 776-788.
  5. Beal, M. (2002). Antioxidant systems in normal pregnancy and pregnancy induced hypertension. American Journal of Obstetrics and Gynecology. 165: 1701-1704.
  6. Buettner, G.R. (1993). The pecking order of free radicals and antioxidants: lipid peroxidation,alpha-tocopherol and ascorbate. Arch. Biochem. Biophys. 300: 535–543.
  7. Catalá, A. (2006). An over view of lipid peroxidation with emphasis in outer segments of photoreceptors and the chemiluminescence assay. Int. J. Biochem. Cell Biol. 38: 1482–1495, Review.
  8. Celec, P., Cervenka, J., Hodosy, P., Vesela, L., Halcak, D., Ostatnikova, A. and Podhradsky, P. (2005).Thiobarbituric acid reacting substances in saliva and their relation to the gingival inflammation. Timisoara Medical Journal. 54: 81-85.
  9. Comporti, M. (1989). Three models of free radical-induced cell injury. Chemico Biological Interactions. 72:1–56.
  10. Derouiche, S., Zeghibek, K., Gharbi, S. and Khelef, Y. (2017). In-vivo study of oxidative stress and liver damage in rats exposed to acetate lead. Int. Res. J. of Bio. Sci. 6(9): 1-6.
  11. Djordjevic, A., Spasic, S., Jovanovic G. A., Djordjevic, R. and Grubor, L. G. (2004). Oxidative stress in diabetic pregnancy: SOD, CAT and GSH-Px activity and lipid peroxidation products. Journal of Maternal-Fetal Neonatal Medicine. 16: 367-372.
  12. Dixit, A., Girling, J. C. (2008). Obesity and pregnancy. Journal of obstetrics and Gynecolog. 28(1):14-23.
  13. Esterbauer, H., Schaur, R.J. and Zollner, H. (1991).Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes.Free Radic. Biol. Medicine. 11: 81–128.
  14. Evans, J. L. (2007).Antioxidants: do they have a role in the treatment of insulin resistance. Indian Journal of Medical Research. 125(12): 355-372.
  15. Fialova, M., Knapen, M. and Peters, W. H. (2006). Glutathione and glutathione related enzymes in reproduction. European Journal of Obstetrics and Gynecology. 82(2): 171-184.
  16. Green, D.R., Reed, J.C. (1998). Mitochondria and apoptosis. Science. 281: 1309–1312.
  17. Gubory, K. H., Bolifraud, P., Gareel, C. (2010). Regulation of key antioxidant enzymatic systems in the sheep endometrium by ovarian steroids.Endocrinology. 128: 2958-2966.
  18. Haddad, J. J. (2002). Antioxidant and prooxidant mechanisms in the regulation of redox sensitive transcription factors.Cell Signalling.14: 879–897.
  19. Halliwell, B. and Gutteridege, J. M. C. (2007). Free radicals in biology and medicine, 4th Edition. Oxford University Press. Grune Strottan, New York.
  20. Hung, T. H., Burton, G. J. (2010). Hypoxia and re oxygenation: a possible mechanism for placental oxidative stress in pre eclampsia. Taiwan Journal of Obstetrics and Gynecology. 45: 189–200.
  21. Kate, Duhig., Lucy Chappell, C., Andrew Shennan, H (2016). Oxidative stress in pregnancy and reproduction. Obstet Med. 9(3): 113–116.
  22. Kehrer, J.P., Biswal, S.S. (2000). The molecular effects of acrolein. Toxicol. Sci. 57: 6–15.
  23. Kim, A., Murphy, M. P., Oberley, T. (2005). Mitochondrial redoxstate regulates transcription of the nuclear encoded mitochondrial protein manganese superoxide dismutase: a proposed adaptative response to mitochondrial redox imbalance. Free Radical Biology and Medicine. 38: 644–654.
  24. Konvicna, J., Vargova, M., Paulikova, I., Kovac, G., Kostecka, Z. (2015). Oxidative stress and antioxidant status in dairy cows during prepartal and postpartalperiods. Acta Vet Brono. 84: 133-140.
  25. Lee, S.H., Oe, T., Blair, I.A. (2001).Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins. Science. 292: 2083–2086.
  26. LPHSI - Livestock and Poultry Heat Stress Indices (1990). Agriculture engineering technology guide.Clemson Univeristy, Clemson, SC, USA.
  27. Marai, I. F. M.,El-Darawany, A. A.,Fadiel, A.,Abdel-Hafez, M. A. M. (2007). Physiological traits as affected by heat stress in sheep: a review. Small Ruminant Research. 71: 1-12.
  28. Marcozzi, G. A. (1996). Rapid procedure for the purification of human salivary peroxidase. Biomed chromatography. 10: 97-98.
  29. Meucci, E., Littarru, C., Deli, G., Luciani, G., Tazza, L., Littarru, G. P. (1998). Antioxidant status and dialysis: plasma and saliva antioxidant activity in patients with fluctuating urate levels. Free Radical Research. 29:367–376.
  30. Myatt, L., Cui, X. (2004). Oxidative stress in the placenta.Cell Biology. 122: 369-382.
  31. Nakai, A., Oya, A., Kobe, H., Asakura, H., Yokota, A., Koshino, T., Araki. T. (2000). Changes in maternal lipid peroxidation levels and antioxidant enzymatic activities before and after delivery. J. of Nippon Medical Sci.67: 434-439.
  32. National Research Council. (2001). Nutrient Requirements of Dairy Cattle: Seventh Revised Edition. The National Academies Press. Washington, DC. ISBN 978-0-309-06997-7.
  33. Nigam, S., Schewe, T. (2000).Phospholipase A2s and lipid peroxidation.Biochim.Biophys. Acta. 1488: 167–181.
  34. Paglia,D.E., Valentine,W.N.(1986). Studies on quantitative and qualitatitve characterization of erythrocyte glutathione peroxidase. Animal Biochemistry. 16: 359-364.
  35. Parola, M., Bellomo, G., Robino, G., Barrera, G., Dianzani, M.U. (1999). 4-Hydroxynonenal as a biological signal: molecular basis and pathophysiological implications. Antioxid.Redox Signal. 1: 255–284.
  36. RT Prasada, Lakshmi Prasanth T, Parvathy R, Murugavel S, Karuna Devi.(2016). Identification of second arginine glycine aspartic acid motif of ovine Vitronectin as the complement C9 binding site and its implication in bacterial infection. Microbiology and Immunology. 61(2): 75 – 84.
  37. Rao, R. K., Thomas, D. W., Pepperl, S., Porreca, F. (1997). Salivary epidermal growth factor plays a role in protection of ileal mucosal integrity. Digestive System Diseases Science. 42: 2175–2181.
  38. Sahoo, S. S., Parta, R. C., Behera, P. C., Swarup, D. (2009). Oxidative stress indices in the erythrocytes from lactating cows after treatment for subclinical ketosis with antioxidant incorporated in the therapeutic regime. Veterinary Research Community. 33: 281-290.
  39. Sharma, N., Singh, N. K., Singh, O.P., Pandey, V., Verma, P. K. (2011). Oxidative stress and antioxidant status during transition period in dairy cows. Aust. J. of Animal Sci. 24(4): 479-484.
  40. Suney L, Y., Oberly L.W., Li Y. (1988). A simple method for clinical assay of superoxide dismutase. Clin. Chem. 34 (3): 497-500.
  41. Uchida, K., (1999). Current status of acrolein as a lipid peroxidation product.Trends Cardiovasc. Med. 9: 109–113.
  42. Vanderlelie, J., Venardos, K., Clifton, V. L., Gude, N. M., Clarke, F. M., Perkins, A. V., (2008). Increased biological oxidation and reduced anti-oxidant enzyme activity in pre-eclamptic placentae. Placenta. 26(1): 53-58.
  43. Wisdom, S. J., Wilson, R., Mckillop, J. H.,Walker, J. J. (1991). Antioxidant systems in normal pregnancy and in pregnancy induced hypertension. Ame. J. of obs. and Gyn. 6(1): 1701-1705.
  44. Yant, L., Ran, J., Rao, Q.,Vanremnen, L., Shibatani, H.T., Belter., J. G., Motta, L., Richardson, A., Prolla, T. A.(2003). The selenoproteinGpx is essential for mouse development and protects from radiation and oxidative damage insults. Reproductive biology. 34: 496–502.

Global Footprints